Bena_00100 : CDS information

Location

Organism	Streptomyces sp.
Strain	A2991200
Entry name	Benastatin
Contig	AM501485
Start / Stop / Direction	8,133 / 9,362 / + [in whole cluster] 8,133 / 9,362 / + [in contig]
Location	8133..9362 [in whole cluster] 8133..9362 [in contig]
Type	CDS
Length	1,230 bp (409 aa)

Annotation

Category	1.1 PKS
Product	polyketide synthase chain length factor subunit
Product (GenBank)	BenB protein
Gene
Gene (GenBank)	benB
EC number
Keyword	type II PKS
Note
Note (GenBank)
Reference	ACC B1GSN2 PmId [17439117] Molecular analysis of the benastatin biosynthetic pathway and genetic engineering of altered fatty acid-polyketide hybrids. (J Am Chem Soc. , 2007) comment Benastatin A and B生合成をコードする全遺伝子座のクローニング、シークエンス。 flanking regionsの削除とS. albus, S. lividansでの異種性発現によってclusterが同定されている。 BenB: chain-length factor(CLF, KSb) 配列解析のみ。
Related Reference	ACC Q54174 NITE Urd_00130 PmId [7592377] Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tu2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation. (J Bacteriol. , 1995) comment Blast 14th, 56%, 1e-122 Streptomyces fradiae_urdB Putative chain length determinant urdEFABCDを含むcosmid purd8をS.lividans TK24へ導入すると、urdamycin抵抗性を獲得。 urdamycin産生株S.fradiae Tu2717染色体から、urdE-C末+urdFABを含むfragment g領域を削除するとurdamycin産生不可。 AA配列解析で、UrdBは芳香族PKS gene clustersのCLFに似ている。 UrdAとUrdBはid36%で似ているが、UrdBはactive site cysteineを持っていない。 TcmK ketoacyl synthase or TcmL chain length factorのどちらかを障害してtetracenomycin C非産生にしたStreptomyces glaucescensのmutantで、urdFABを異種性発現したらどちらのmutantも相補され、tetracenomycin Cを産生できた。

PKS/NRPS Module

CLF	3..359

Sequence

>polyketide synthase chain length factor subunit [BenB protein]
MTVITGLGVVAPTGVGLDDYWATTLAGKSGIDRIRRFDPSGYTAQLAGQVDDFEATDHVP
SKLLAQTDRMTHFAFAGANMALADAHVDLADFPEYERAVVTANSSGGVEYGQHELQKMWS
GGPMRVSAYMSVAWFYAATTGQLSIHHGLRGPCGLIATEQAGGLDALGHARRLLRRGARI
AVTGGTDAPLSPASMVAQLATGLLSSNPDPTAAYLPFDDRAAGYVPGEGGAIMIMEPAEH
ALRRGAERIYGEIAGYAATFDPAPGTGRGPTLGRAIRNALDDARIAPSEVDLVFADGSGT
PAMDRAEAEALTEVFGPRGVPVTVPKAATGRMYSGGGALDVATALLAMRDGVAPPTPHVT
ELASDCPLDLVRTEPRELPIRHALVCARGVGGFNAALVLRRGDLTTPEH

>polyketide synthase chain length factor subunit [BenB protein]
ATGACGGTGATCACCGGTCTGGGCGTGGTCGCCCCCACGGGCGTGGGCCTGGACGACTAC
TGGGCCACCACACTGGCGGGCAAGAGCGGCATCGACCGTATCCGCCGCTTCGACCCCTCC
GGCTACACCGCTCAACTCGCCGGTCAGGTCGACGACTTCGAGGCGACCGACCACGTCCCC
TCCAAGCTGCTCGCACAGACCGACCGGATGACGCACTTCGCGTTCGCCGGTGCGAACATG
GCGTTGGCGGACGCGCATGTCGACCTTGCCGACTTTCCCGAGTACGAGCGGGCCGTGGTC
ACCGCCAACTCCTCCGGGGGCGTCGAGTACGGGCAGCACGAACTGCAGAAGATGTGGAGT
GGCGGCCCCATGCGGGTGAGCGCCTACATGTCGGTGGCCTGGTTCTACGCGGCCACCACC
GGTCAGCTGTCCATCCATCACGGCCTGCGTGGCCCCTGCGGGCTGATAGCGACCGAACAG
GCCGGGGGACTCGATGCCCTCGGACACGCCCGGCGTCTGCTGCGGCGCGGCGCCCGCATC
GCCGTCACGGGTGGCACCGACGCGCCGCTCTCGCCGGCCAGCATGGTCGCGCAGTTGGCG
ACCGGGCTGCTCAGTTCGAACCCGGACCCGACGGCCGCCTACCTGCCGTTCGACGACCGT
GCGGCGGGCTACGTGCCGGGCGAGGGCGGCGCGATCATGATCATGGAACCGGCCGAGCAC
GCACTGCGGCGCGGTGCCGAGCGGATCTACGGAGAGATCGCCGGGTACGCGGCGACCTTC
GACCCCGCGCCGGGCACCGGACGGGGCCCGACACTCGGCCGGGCGATCCGTAACGCGCTC
GACGACGCCCGGATCGCCCCAAGCGAAGTCGACCTGGTCTTCGCGGACGGCTCCGGGACG
CCGGCCATGGACCGCGCGGAGGCCGAAGCGCTGACGGAGGTGTTCGGGCCGCGCGGTGTA
CCGGTCACCGTCCCGAAGGCGGCAACCGGGCGGATGTACTCCGGCGGTGGCGCCCTCGAT
GTCGCCACCGCTCTGTTGGCCATGCGCGACGGCGTGGCGCCGCCCACCCCGCATGTGACC
GAGCTCGCCTCGGACTGCCCGCTGGATCTTGTCCGTACGGAGCCGCGGGAGCTGCCGATC
CGTCATGCATTGGTCTGCGCCAGGGGAGTTGGCGGCTTCAACGCGGCTCTCGTACTGCGG
CGCGGTGACCTGACGACTCCGGAGCACTGA

CLF	3..359

CLF	7..1077

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR014030 Beta-ketoacyl synthase, N-terminal (Domain) PF00109 ketoacyl-synt IPR014031 Beta-ketoacyl synthase, C-terminal (Domain) PF02801 Ketoacyl-synt_C IPR016038 Thiolase-like, subgroup (Domain) G3DSA:3.40.47.10 Thiolase-like_subgr IPR016039 Thiolase-like (Domain) SSF53901 Thiolase-like
SignalP	Eukaryota
TMHMM	No significant hit

Bena_00090 Bena_00110