Bena_00080 Bena_00080   Bena_00100 Bena_00100

Bena_00090 : CDS information

close this sectionLocation

Organism
StrainA2991200
Entry nameBenastatin
Contig
Start / Stop / Direction6,865 / 8,136 / + [in whole cluster]
6,865 / 8,136 / + [in contig]
Location6865..8136 [in whole cluster]
6865..8136 [in contig]
TypeCDS
Length1,272 bp (423 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase beta-ketoacyl synthase subunit
Product (GenBank)BenA protein
Gene
Gene (GenBank)benA
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[17439117] Molecular analysis of the benastatin biosynthetic pathway and genetic engineering of altered fatty acid-polyketide hybrids. (J Am Chem Soc. , 2007)
comment
Benastatin A and B生合成をコードする全遺伝子座のクローニング、シークエンス。
flanking regionsの削除とS. albus, S. lividansでの異種性発現によってclusterが同定されている。

BenA: ketosynthase(KSa)
配列解析のみ。
Related Reference
ACC
Q54173
NITE
Urd_00120
PmId
[7592377] Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tu2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation. (J Bacteriol. , 1995)
comment
Blast 3rd, id70%, 1e-169
Streptomyces fradiae_urdA
Putative ketoacyl synthase

urdEFABCDを含むcosmid purd8をS.lividans TK24へ導入すると、urdamycin抵抗性を獲得。
urdamycin産生株S.fradiae Tu2717染色体から、urdE-C末+urdFABを含むfragment g領域を削除するとurdamycin産生不可。

AA配列解析で、UrdAはPKS gene clustersのbeta-ketoacyl synthasesに高い類似性あり。
UrdAとUrdBはid36%で似ているが、UrdBにはないactive site cysteineをUrdAは持っている。

TcmK ketoacyl synthase or TcmL chain length factorのどちらかを障害してtetracenomycin C非産生にしたStreptomyces glaucescensのmutantで、urdFABを異種性発現したらどちらのmutantも相補され、tetracenomycin Cを産生できた。

close this sectionPKS/NRPS Module

KS5..376

close this sectionSequence

selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [BenA protein]
MSSERRAVITGMGVIAPGGVGTRAFWSAVTAGRTATRRITLFDPERFRCRIAAECDFDAA
ALGLTPQEIRRMDRAVQMAVAATGEALADAGVGEGDLDPARTGVTIGNAVGSTMMMEEEY
VVISDGGRKWLCDEEYGVRHLYGAVIPSTAGVEVARRVGAEGPTAVVSTGCTSGLDAVGH
AAQLIEEGSADVVIGGATDAPISPITVACFDSLKATSTRNDDAEHACRPFDRDRDGLVLG
EGSAVFVMEARERAVRRGAKIYCEVAGYAGRANAYHMTGLKPDGRELAEAIDRAMAQAGI
SAEDIDYVNAHGSGTRQNDRHETAAFKRSLRDHARRVPVSSIKSMVGHSLGAIGAIEVAA
SALAIEHGVVPPTANLTTPDPECDLDYVPREAREHPTDVVLSVGSGFGGFQSAVVLISPR
SRR
selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [BenA protein]
ATGAGCAGTGAACGACGTGCCGTCATCACCGGGATGGGAGTCATAGCTCCAGGCGGGGTC
GGTACCCGCGCCTTCTGGTCCGCGGTGACCGCGGGCCGGACGGCCACGCGCCGGATCACC
CTGTTCGACCCGGAGCGGTTCCGCTGCCGGATCGCGGCGGAGTGCGACTTCGACGCGGCC
GCACTGGGCCTGACTCCACAGGAGATCCGCCGGATGGACCGGGCCGTGCAGATGGCGGTG
GCCGCCACCGGCGAGGCACTCGCGGACGCGGGGGTCGGCGAGGGCGACCTGGATCCGGCC
CGTACCGGTGTCACCATCGGCAACGCGGTCGGCAGCACGATGATGATGGAGGAGGAGTAC
GTCGTCATCAGCGACGGCGGCCGCAAGTGGCTGTGCGACGAGGAATATGGCGTACGGCAC
CTGTACGGCGCGGTCATCCCGAGTACCGCAGGGGTGGAGGTGGCGCGTCGCGTCGGCGCG
GAGGGACCGACGGCGGTGGTGTCGACCGGCTGCACGTCCGGGCTCGACGCGGTCGGGCAC
GCCGCGCAGCTGATCGAGGAGGGCTCCGCGGACGTGGTGATCGGTGGTGCCACGGACGCC
CCGATCTCGCCGATCACGGTGGCCTGCTTCGACTCGCTGAAGGCCACCTCGACCCGCAAC
GACGACGCGGAACACGCCTGCCGCCCCTTCGACCGGGACCGCGACGGGCTGGTGCTGGGC
GAGGGGTCCGCGGTCTTCGTGATGGAGGCGAGGGAGCGGGCCGTACGGCGCGGCGCGAAG
ATCTACTGTGAGGTGGCCGGCTACGCCGGACGTGCCAACGCCTATCACATGACCGGTCTG
AAGCCGGACGGCCGGGAACTGGCCGAGGCCATCGACCGGGCCATGGCGCAGGCCGGCATC
TCGGCCGAGGACATCGACTACGTCAACGCGCACGGATCGGGCACGCGGCAGAACGACCGG
CACGAGACAGCCGCGTTCAAGCGAAGCCTGAGGGACCACGCCCGCCGTGTCCCGGTCAGC
TCGATCAAGTCGATGGTGGGGCATTCCCTGGGCGCCATAGGCGCGATCGAGGTGGCCGCC
TCCGCACTCGCCATCGAGCACGGAGTGGTGCCGCCGACGGCGAACCTCACCACACCGGAC
CCCGAGTGCGATCTCGACTATGTCCCGCGCGAGGCGCGTGAGCACCCCACCGACGTGGTG
CTCAGCGTCGGAAGCGGCTTCGGCGGCTTCCAGAGCGCGGTCGTACTGATCTCTCCGAGG
AGCAGGCGATGA
KS5..376
KS13..1128

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[5-252] 4.30000000000003e-55 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[262-376] 1.9e-38 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[3-267] 2.1e-63 G3DSA:3.40.47.10 [273-416] 1e-55 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[1-421] 4.89996723796399e-70 SSF53901
SSF53901   Thiolase-like
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[162-178] PS00606
PS00606   B_KETOACYL_SYNTHASE
SignalP No significant hit
TMHMM
[7-29] Transmembrane (i-o)
Transmembrane 1   
Bena_00080 Bena_00080   Bena_00100 Bena_00100
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