Chalc_00370 Chalc_00370   Chalc_00390 Chalc_00390

Chalc_00380 : CDS information

close this sectionLocation

Organism
StrainNRRL 2737
Entry nameChalcomycin
Contig
Start / Stop / Direction79,983 / 79,228 / - [in whole cluster]
79,983 / 79,228 / - [in contig]
Locationcomplement(79228..79983) [in whole cluster]
complement(79228..79983) [in contig]
TypeCDS
Length756 bp (251 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative type II thioesterase
Product (GenBank)thioesterase TEII family
Gene
Gene (GenBank)ORF13
EC number
Keyword
Note
  • It is supposed that this ORF is not involved in chalcomycin biosynthesis.
Note (GenBank)
  • similar to RifR
Reference
ACC
PmId
[15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004)
comment
Chalcomycin biosynthesis gene clusterに関する文献。
ORF13(251a.a.): Thioesterase (TEII family)

ORF13よりもChmIのほうがtype II thioesterasesのfamilyにより似ているので、chalcomycin生合成においてはORF13ではなくChmIがtype II TEとして働くと提唱されている。
Related Reference
ACC
Q7BUF9
NITE
Rifam_00540
PmId
[19103602] Structure and functional analysis of RifR, the type II thioesterase from the rifamycin biosynthetic pathway. (J Biol Chem. , 2009)
comment
8th(Q7BUF9) 45%, 1e-51
Amycolatopsis mediterranei_rifR
[Rifam_00540]type II thioesterase

RifRは、acyl-CoAsよりacyl carrier domainのphosphopantetheinyl armからのacyl unitsを加水分解する(Steady-state kinetics)。
helical lidの運動が、RifR active siteへの基質のアクセスを調節する(立体構造解析)。

RifRはCoA基質よりACP基質への反応を好む。これはtype II thioesteraseがcarrier domainsから異常unitsを除くようなhousekeeping functionsをすると考えられていることに一致する。

close this sectionSequence

selected fasta
>putative type II thioesterase [thioesterase TEII family]
MTTGTDSTTWFRRYSSTPAPRRRLAVLPHAGGSASFFHAWGSAFGGDTEVLVAQYPGRQE
RFNEPFVDRMDVLADRVTAALLPLADVPLTLFGHSMGASLAYEVALRLEERHRVTPAALH
VSSRKAPHRLTPLDLHRKGDDELVAVLRGLGGTDTALLDDPDIRQLVLPAVRADFTVVST
YGPRVPTAVGCPVHAWIGDTDPNVAVGDMDAWADVAPEGFRVRVLPGGHFYLLQQHETLM
RELSGHLAGDR
selected fasta
>putative type II thioesterase [thioesterase TEII family]
ATGACGACCGGCACGGACAGCACGACGTGGTTCCGCAGGTACTCCAGCACTCCCGCGCCC
CGGCGGCGGCTGGCGGTCCTGCCGCACGCGGGCGGTTCCGCGAGCTTCTTCCACGCCTGG
GGCAGCGCCTTCGGCGGCGACACCGAGGTCCTGGTGGCGCAGTACCCCGGACGCCAGGAG
CGGTTCAACGAGCCGTTCGTCGACCGGATGGACGTGCTCGCCGACCGGGTCACGGCGGCA
CTGCTGCCGCTCGCGGACGTGCCCCTGACGCTCTTCGGCCACAGCATGGGCGCGTCCCTG
GCCTACGAGGTGGCGCTGCGCCTGGAGGAGCGGCACCGCGTCACGCCCGCCGCCCTCCAC
GTCTCCAGCCGCAAGGCCCCGCACCGGCTGACCCCGCTCGACCTGCACCGGAAGGGGGAC
GACGAGCTGGTCGCCGTACTGCGCGGGCTCGGCGGGACCGATACCGCCCTGCTTGACGAC
CCGGACATCCGACAGCTCGTGCTGCCCGCGGTCCGGGCGGACTTCACCGTGGTGAGCACC
TACGGCCCCCGCGTCCCCACCGCCGTCGGCTGTCCGGTGCACGCCTGGATCGGCGACACG
GATCCGAACGTGGCGGTAGGGGACATGGACGCCTGGGCGGATGTCGCACCCGAGGGCTTC
CGGGTACGGGTCCTGCCCGGCGGCCACTTCTACCTGCTCCAGCAGCACGAGACGCTGATG
CGCGAGCTCTCGGGGCACCTGGCCGGAGACCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001031 Thioesterase (Domain)
[22-244] 1.6e-49 PF00975
PF00975   Thioesterase
SignalP
[1-39] 0.414 Signal
Bacteria, Gram-positive   
[1-45] 0.496 Signal
Eukaryota   
TMHMM No significant hit
Chalc_00370 Chalc_00370   Chalc_00390 Chalc_00390
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