Chart_00020 : CDS information

Location

Organism	Streptomyces chartreusis
Strain	HKI-249
Entry name	Chartreusin
Contig	AJ786382
Start / Stop / Direction	2,233 / 1,460 / - [in whole cluster] 2,233 / 1,460 / - [in contig]
Location	complement(1460..2233) [in whole cluster] complement(1460..2233) [in contig]
Type	CDS
Length	774 bp (257 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	ChaM protein
Gene
Gene (GenBank)	chaM
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q4R0N0 PmId [15911378] Biosynthesis of the antitumor agent chartreusin involves the oxidative rearrangement of an anthracyclic polyketide. (Chem Biol. , 2005) comment chartreusin生合成clusterの解析論文。異種性にclusterを発現してchartreusin産生を確認。 ChaM: SAM-dependent methyl transferase 配列解析に基づき、chartreusinに含まれる2つのdeoxysugarsのうちD-digitaloseの生合成に関与し、 D-fucoseの3-O-methylationを担うと考えられている。

Sequence

>hypothetical protein [ChaM protein]
MDTSMYHAFAAVESRHWWFQGRRRIVAALLRDQLRDVRRAKPGGLDILDVGSGTGEMVEM
LREFGTVTALDSSPHAIDYLRQRLPEGVSARHGSIPEDLPRDEMFDLVTAFDVVEHLNDD
LAALRAFRQVLPTGGRMALTVPAYQMLWSHHDVYAGHVRRYRRAQLRDLVQRAGGFQVER
LAYFNSLLFLPALAARSAGRWRRRVSPRVAERVDPHLALPSPLVNRLLLGVFSSEAGLLS
RVDPPFGVSLVALCRAC

>hypothetical protein [ChaM protein]
ATGGACACCTCCATGTACCACGCGTTCGCCGCGGTGGAGAGCCGGCACTGGTGGTTCCAG
GGGCGTCGGCGGATCGTCGCCGCGCTGCTGCGGGACCAGCTGAGGGATGTCCGGCGAGCG
AAGCCCGGTGGGCTGGACATCCTCGACGTCGGGTCGGGGACGGGCGAAATGGTCGAGATG
CTGCGCGAGTTCGGGACCGTCACCGCACTCGACAGTTCCCCGCACGCCATCGACTACCTG
CGGCAGAGACTGCCCGAGGGTGTGTCGGCCCGGCACGGCAGCATCCCCGAGGATCTGCCC
CGCGACGAGATGTTCGACCTGGTGACCGCCTTCGACGTGGTCGAGCACCTCAACGACGAC
CTGGCGGCGCTGCGCGCGTTCCGCCAAGTACTGCCCACGGGCGGCCGCATGGCGCTCACC
GTCCCCGCCTACCAGATGCTGTGGAGCCACCACGACGTCTACGCCGGGCACGTCAGGCGC
TACCGCCGTGCGCAGCTGCGGGATCTCGTGCAGCGGGCCGGCGGCTTCCAGGTGGAACGC
CTCGCCTACTTCAACTCGCTGCTGTTCCTGCCGGCCCTCGCGGCGAGGTCGGCGGGCCGC
TGGCGGCGCCGGGTGTCGCCACGCGTGGCCGAGCGCGTCGATCCGCATCTGGCCCTGCCT
TCCCCGCTGGTGAACCGGCTTCTGCTCGGGGTCTTCTCCTCCGAGGCCGGTCTGCTGTCA
CGCGTCGACCCGCCGTTCGGCGTCTCGCTCGTCGCGCTGTGCCGCGCCTGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

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