Chart_00080 : CDS information

Location

Organism	Streptomyces chartreusis
Strain	HKI-249
Entry name	Chartreusin
Contig	AJ786382
Start / Stop / Direction	6,565 / 7,056 / + [in whole cluster] 6,565 / 7,056 / + [in contig]
Location	6565..7056 [in whole cluster] 6565..7056 [in contig]
Type	CDS
Length	492 bp (163 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	ChaS3 protein
Gene
Gene (GenBank)	chaS3
EC number
Keyword
Note	The N-terminal position was modified from original INSDC entry.
Note (GenBank)
Reference	ACC Q4R0M4 PmId [15911378] Biosynthesis of the antitumor agent chartreusin involves the oxidative rearrangement of an anthracyclic polyketide. (Chem Biol. , 2005) comment chartreusin生合成clusterの解析論文。異種性にclusterを発現してchartreusin産生を確認。 ChaS3: NDP-4-Keto-6-deoxy-D-glucose reductase 配列解析に基づき、機能予測している。 chartreusinに含まれる2つのdeoxysugars(D-fucose, D-digitalose)の生合成に関与し、NDP-4-keto-6-deoxy-D-glucose → NDP-D-fucoseを触媒するreductaseとして機能すると提唱されている。

Sequence

>hypothetical protein [ChaS3 protein]
MTSPPDGGAEQGAPAGRRHAPLLGELARFGVMGVVNSTVAIAGTSVLHVGLRVGPLTSNA
IATLLATTLSFAGNRWWTFRHRRRSNLVQEYALFFVLNGAGLLIQLLAIAFAVRVLDRDD
PVSYHVALLVGVAVASLVRFLSYKKWVFLPRERPVRAGARRPG

>hypothetical protein [ChaS3 protein]
ATGACATCGCCTCCTGACGGCGGCGCCGAACAGGGCGCGCCGGCAGGCCGACGGCACGCG
CCGCTCCTCGGTGAACTCGCCCGCTTCGGAGTGATGGGAGTCGTCAACTCCACGGTGGCG
ATAGCCGGCACCAGCGTGCTCCATGTCGGCCTGCGGGTGGGGCCGCTCACCTCGAACGCG
ATCGCCACCCTGCTCGCGACGACCCTGTCGTTCGCCGGGAACCGCTGGTGGACCTTCCGG
CACCGCAGACGCAGCAACCTCGTCCAGGAGTACGCCCTCTTCTTCGTCCTGAACGGCGCC
GGCCTGCTGATCCAGCTCCTCGCCATCGCGTTCGCCGTCCGGGTACTGGACCGGGACGAC
CCGGTGTCGTACCACGTGGCGCTGCTCGTCGGGGTGGCCGTGGCCTCGCTGGTCCGCTTC
CTCAGCTACAAGAAGTGGGTGTTCCTCCCGAGGGAGCGGCCGGTCCGTGCGGGTGCGCGC
AGGCCCGGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR007267 GtrA-like protein (Domain) PF04138 GtrA
SignalP	No significant hit
TMHMM	Transmembrane 3

Chart_00070 Chart_00090