Chart_00090 : CDS information

Location

Organism	Streptomyces chartreusis
Strain	HKI-249
Entry name	Chartreusin
Contig	AJ786382
Start / Stop / Direction	7,915 / 7,130 / - [in whole cluster] 7,915 / 7,130 / - [in contig]
Location	complement(7130..7915) [in whole cluster] complement(7130..7915) [in contig]
Type	CDS
Length	786 bp (261 aa)

Annotation

Category	4.1 transcriptional regulator
Product	putative transcriptional regulator
Product (GenBank)	ChaR1 protein
Gene
Gene (GenBank)	chaR1
EC number
Keyword	SARP family
Note	The N-terminal position was modified from original INSDC entry.
Note (GenBank)
Reference	ACC Q4R0M3 PmId [15911378] Biosynthesis of the antitumor agent chartreusin involves the oxidative rearrangement of an anthracyclic polyketide. (Chem Biol. , 2005) comment chartreusin生合成clusterの解析論文。異種性にclusterを発現してchartreusin産生を確認。 ChaR1: SARP family regulator 配列解析に基づき、機能予測している。 grhR2, aknOなどpathway-specific SARP regulator genesに似ている。
Related Reference	ACC Q9XCC4 PmId [11985721] Differential roles of two SARP-encoding regulatory genes during tylosin biosynthesis. (Mol Microbiol. , 2002) [16942604] Regulation of tylosin biosynthesis involving 'SARP-helper' activity. (Mol Microbiol. , 2006) comment Blast 16th, id50%, 8e-62 Streptomyces fradiae_tylS Hypothetical pathway specific regulatory protein TylS --- [PMID:11985721] tylSはtylosin産生に必須。 TysSはtylRなどの遺伝子発現を調節する。 -- [PMID:16942604] TylSでtylUは調節される。 tylU破壊でTylRが減少し、tylosin収量が80%になる。 TylS and TylUは、tylRの発現を促進するために協調して働く。
Related Reference	ACC P25047 NITE Adria_00210 PmId [1729206] Regulation of secondary metabolism in Streptomyces spp. and overproduction of daunorubicin in Streptomyces peucetius. (J Bacteriol. , 1992) [7868593] Functional characterization and transcriptional analysis of the dnrR1 locus, which controls daunorubicin biosynthesis in Streptomyces peucetius. (J Bacteriol. , 1995) [8971703] Purification and characterization of the DNA-binding protein DnrI, a transcriptional factor of daunorubicin biosynthesis in Streptomyces peucetius. (Mol Microbiol. , 1996) [16545946] Feedback regulation of doxorubicin biosynthesis in Streptomyces peucetius. (Res Microbiol. , 2006) [20529134] Modulation of dnrN expression by intracellular levels of DnrO and daunorubicin in Streptomyces peucetius. (FEMS Microbiol Lett. , 2010) [20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010) comment Blast 19th, id47%, 3e-61 Streptomyces peucetius_dnrI Regulatory protein dnrI -- [PMID: 1729206](1992) ATCC 29050 strainでdnrI を不活化したら、RHO産生とdaunorubicin (DNR)産生ができなくなり、DNRへの抵抗性が著しく減った。 --- 同じ著者らの続報。 [PMID: 7868593](1995) dnrI::aphII mutantでは、DNR生合成におけるearly- and late-acting enzymesの両方をコードする転写物が存在しない。よってdnrI は、直接または間接的にDNR生合成遺伝子のすべてを調節している。 dnrI::aphII and dnrN::aphII strainsでresistance genes=drrAB and drrC転写物は消失。よって、dnrI はDNR抵抗性の重大なregulatorであり、dnrNはdnrI を通して間接的にその影響を発現する、と示唆される。 -- [PMID: 8971703](1996) abstract DNA-binding assays. ・dnrG-dpsABCD と dpsEF の間・dnrC gene と dnrDKPSQ の間の遺伝子間領域を含んだDNA断片へ特異的に結合する。 -- [PMID: 16545946](2006) abstract dnrI promoter活性を増やすためにはDnrO and DnrN の両方が必要であった。 -- [PMID: 20529134]や[PMID: 20158521]で、dnrO, dnrN, dnrI, DNRが関与する細胞内DNR濃度維持システムが提唱されている。 dnrI promoterにはDnrNが結合し活性化される。

Sequence

>putative transcriptional regulator [ChaR1 protein]
MEIRILGPISIEPIGTVSTRLAPKPRKILALLLLNANKIVSTASLMTELWGETPPKSATT
TLQTYVLHLRKALGALWEIDLPDIAKNVLTTHTNGYMLSISPGTVDLHVYNKLVADGRRA
LEEGQIERGAARFDEALALWSGPPLADVPIGPLLEAEVMRLEEDRLGILEDRISADLRLG
RHHQLLGELTSLVAQHPFHEGLHEKLMVALHRSGRRHQALDVYQRLRSSLVDELGIDPSP
RLQRLHQSVLASDPRLDNAAA

>putative transcriptional regulator [ChaR1 protein]
TTGGAGATACGAATACTCGGCCCCATATCCATCGAACCGATCGGGACGGTGAGCACTCGA
CTCGCCCCCAAGCCACGCAAGATCCTGGCTCTGCTGCTCCTCAACGCGAACAAGATCGTA
TCGACTGCTTCACTCATGACCGAGCTCTGGGGCGAGACCCCGCCCAAGAGCGCGACCACG
ACCTTGCAGACCTATGTCCTGCACCTGCGCAAGGCACTTGGCGCCCTATGGGAAATCGAT
CTGCCTGACATAGCCAAGAACGTGCTCACCACACACACCAACGGCTACATGCTCAGCATC
TCGCCCGGGACGGTCGACCTGCATGTGTACAACAAGCTCGTGGCCGATGGCCGGCGTGCC
CTGGAAGAAGGGCAGATCGAACGCGGGGCCGCCCGTTTCGACGAGGCGCTGGCACTGTGG
AGCGGTCCCCCGCTCGCCGACGTGCCCATCGGTCCGCTGCTGGAGGCGGAGGTGATGCGT
CTGGAGGAGGACCGGCTCGGCATCCTGGAGGACCGGATCAGCGCGGACCTCCGGCTTGGC
CGGCACCATCAACTGCTGGGCGAACTGACCTCGTTGGTCGCACAGCATCCCTTCCACGAG
GGTCTGCACGAGAAGCTGATGGTGGCGCTGCACCGGTCCGGCCGGCGGCACCAGGCCCTG
GACGTCTATCAGCGTCTGCGCTCGTCGCTCGTGGACGAGCTCGGCATCGATCCGTCGCCC
AGGCTCCAGCGTCTGCACCAGTCCGTCCTCGCCTCCGACCCGAGGCTGGACAACGCGGCG
GCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001867 Signal transduction response regulator, C-terminal (Domain) SM00862 Transcriptional regulatory protein, C ter PF00486 Trans_reg_C IPR005158 Bacterial transcriptional activator domain (Domain) PF03704 BTAD SM01043 Bacterial transcriptional activator domai IPR011990 Tetratricopeptide-like helical (Domain) G3DSA:1.25.40.10 no description IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 no description IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 C-terminal effector domain of the bipartite response regulators
SignalP	No significant hit
TMHMM	No significant hit

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