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Chart_00100 : CDS information

close this sectionLocation

Organism
StrainHKI-249
Entry nameChartreusin
Contig
Start / Stop / Direction8,278 / 9,546 / + [in whole cluster]
8,278 / 9,546 / + [in contig]
Location8278..9546 [in whole cluster]
8278..9546 [in contig]
TypeCDS
Length1,269 bp (422 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase beta-ketoacyl synthase subunit
Product (GenBank)ChaA protein
Gene
Gene (GenBank)chaA
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[15911378] Biosynthesis of the antitumor agent chartreusin involves the oxidative rearrangement of an anthracyclic polyketide. (Chem Biol. , 2005)
comment
chartreusin生合成clusterの解析論文。
異種性にclusterを発現してchartreusin産生を確認。

ChaA: Ketoacyl synthase

配列解析に基づき、機能予測している。
ChaA and ChaBのAA配列はdecaketides生合成PKSに似ているので、chartreusin aglycone骨格もdecaketideに由来すると考えられている。
Related Reference
ACC
Q54173
NITE
Urd_00120
PmId
[7592377] Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tu2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation. (J Bacteriol. , 1995)
comment
Blast 2nd, id76%, 0.0
Streptomyces fradiae_urdA
Putative ketoacyl synthase
[DoBISCUIT]polyketide synthase beta-ketoacyl synthase subunit

urdEFABCDを含むcosmid purd8をS.lividans TK24へ導入すると、urdamycin抵抗性を獲得。
urdamycin産生株S.fradiae Tu2717染色体から、urdE-C末+urdFABを含むfragment g領域を削除するとurdamycin産生不可。

AA配列解析で、UrdAはPKS gene clustersのbeta-ketoacyl synthasesに高い類似性あり。
UrdAとUrdBはid36%で似ているが、UrdBにはないactive site cysteineをUrdAは持っている。

TcmK ketoacyl synthase or TcmL chain length factorのどちらかを障害してtetracenomycin C非産生にしたStreptomyces glaucescensのmutantで、urdFABを異種性発現したらどちらのmutantも相補され、tetracenomycin Cを産生できた。

close this sectionPKS/NRPS Module

KS5..375

close this sectionSequence

selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [ChaA protein]
MDYCVAITGIGVRAPGGIGTNAFWELLTSGRTATRTISFFDPSPFRSRVAAECDFDARAE
GFTPKELRRMDRAAQLGVAAAREALADSGLGGGGLDPADVSVTVGSAVGCTMGLEQEYST
VSDGGRQWLVDHTYAVPHLYGHFVPSSLAAEVAWAVAAEGPVSVVSTGCTSGLDAIGHAC
GLLREGAGKVAVAGATDAPISPITVACFDAIKATTARNDDPEHASRPFDRSRDGFVLGEG
SAFFVLEPLEDARRRGAHVYATIRGFASRSNAYHMTGLRPDGREMAEAITAALGQARLRP
DDIDYVNAHGSGTRQNDRHETAAVKRSLGAHAFDVPVSSIKSMIGHSLGAIGAIEIAACA
LAIEHDVIPPTANLEEPDPDCDLDYVPKTAREQRTDAVLTVGSGFGGFQSAMVLAAPGAA
AR
selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [ChaA protein]
ATGGACTACTGTGTGGCGATAACCGGAATCGGAGTCCGGGCGCCAGGGGGAATCGGCACC
AATGCGTTCTGGGAACTCCTGACGTCCGGTCGTACCGCAACACGCACGATATCTTTCTTC
GACCCCTCCCCGTTCCGGTCCCGCGTCGCGGCCGAGTGCGATTTCGACGCGCGCGCCGAG
GGCTTCACGCCCAAGGAACTGAGGAGGATGGACCGCGCCGCGCAACTGGGCGTCGCGGCC
GCCCGCGAGGCCCTCGCCGACAGCGGCCTGGGTGGCGGCGGGCTGGATCCGGCGGACGTG
TCCGTCACGGTGGGCAGCGCCGTCGGTTGCACCATGGGCCTGGAACAGGAGTACTCGACC
GTCAGCGACGGCGGCCGGCAGTGGCTCGTCGACCACACCTACGCCGTGCCGCACCTGTAC
GGCCACTTCGTCCCGAGCTCGCTGGCCGCCGAAGTGGCCTGGGCCGTGGCGGCCGAGGGA
CCCGTGTCGGTCGTCTCGACGGGATGCACCTCCGGGCTCGACGCGATCGGCCATGCGTGC
GGGCTGCTGCGGGAGGGAGCCGGGAAGGTCGCGGTGGCCGGTGCCACCGACGCGCCGATC
TCCCCGATCACCGTCGCCTGCTTCGACGCCATCAAGGCCACCACGGCACGCAACGACGAC
CCGGAGCACGCATCCCGGCCCTTCGACCGCAGCCGGGACGGCTTCGTGCTGGGCGAGGGG
AGCGCGTTCTTCGTCCTGGAGCCGCTGGAGGACGCTCGGCGCCGAGGGGCCCATGTGTAC
GCCACGATCCGGGGGTTCGCGTCCCGGTCGAACGCCTATCACATGACCGGTCTGCGGCCG
GACGGCCGGGAGATGGCGGAGGCGATCACCGCGGCACTCGGACAGGCGCGGTTGCGGCCC
GACGACATCGACTACGTCAACGCGCACGGCTCCGGCACCAGGCAGAACGACCGGCACGAG
ACGGCGGCGGTCAAGCGCAGCCTCGGCGCGCACGCCTTCGACGTCCCGGTCAGTTCCATC
AAGTCGATGATCGGGCATTCGCTGGGTGCCATCGGGGCCATCGAGATCGCGGCGTGTGCG
CTGGCCATCGAGCACGACGTCATCCCACCCACAGCCAACCTGGAGGAACCCGACCCCGAC
TGCGACCTCGACTACGTTCCCAAGACCGCGCGCGAGCAGCGGACCGACGCCGTGCTGACG
GTGGGCAGCGGCTTCGGCGGATTCCAGAGCGCGATGGTGCTCGCCGCTCCGGGAGCGGCC
GCCCGATGA
KS5..375
KS13..1125

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[5-252] 5.19999999999996e-60 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[260-375] 3.40000000000004e-40 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[5-265] 1.29999999999998e-64 G3DSA:3.40.47.10 [270-416] 2.29999999999998e-55 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[2-419] 6.69996745106047e-70 SSF53901
SSF53901   Thiolase-like
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[160-176] PS00606
PS00606   B_KETOACYL_SYNTHASE
SignalP No significant hit
TMHMM No significant hit
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