Chart_00100 Chart_00100   Chart_00120 Chart_00120

Chart_00110 : CDS information

close this sectionLocation

Organism
StrainHKI-249
Entry nameChartreusin
Contig
Start / Stop / Direction9,543 / 10,769 / + [in whole cluster]
9,543 / 10,769 / + [in contig]
Location9543..10769 [in whole cluster]
9543..10769 [in contig]
TypeCDS
Length1,227 bp (408 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase chain length factor subunit
Product (GenBank)ChaB protein
Gene
Gene (GenBank)chaB
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[15911378] Biosynthesis of the antitumor agent chartreusin involves the oxidative rearrangement of an anthracyclic polyketide. (Chem Biol. , 2005)
comment
chartreusin生合成clusterの解析論文。
異種性にclusterを発現してchartreusin産生を確認。

ChaB: Chain length factor

配列解析に基づき、機能予測している。
ChaA and ChaBのAA配列はdecaketides生合成PKSに似ているので、chartreusin aglycone骨格もdecaketideに由来すると考えられている。
Related Reference
ACC
Q56164
NITE
Jado_00090
PmId
[7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
Blast 6th, id71%, 1e-155
Streptomyces venezuelae_jadB
Chain length determinant

---
[PMID: 7881555](1994)
ORF2(=JadBに相当)は配列解析からtype II PKSのCLFであると提唱されている。
ORF2-ORF4領域を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。

--
[PMID: 9224566](1997)
minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。
jadBもKS-betaとして使用されている。

close this sectionPKS/NRPS Module

CLF4..362

close this sectionSequence

selected fasta
>polyketide synthase chain length factor subunit [ChaB protein]
MSAKVVVTGLGVAAPNGLGTRAYWEATLRGRSGIAPLKRFDARGYPARLAGEVPGFEPAE
HLPGRLLPQTDHMTRMALVAADWALADARADPERLAEFSMGVVTASSSGGFEFGQRELEK
LWSKGSRYVSSYQSFAWFYAVNTGQISIRNGMRGPSGVLVSDQAGGLDAVAQARRLVREG
LALVVSGGVDASLCPWGWVAQLSTGRLSTGDDPRSAYLPFSAAARGYVPGEGGAILVLEE
EGAAARRRPGAPYGEIAGYGATFDPHPDSGRPPRLRDAIEIALGDAGLDPSDVDVLFADA
AGLPDLDRVEAEAISDVFGPRGVPVTAPKTMTGRLYSGGAALDLAAALLSLNEGVVPPTV
NVGRPAPEYAIDLVVRPRPAPLRVALVLARGHGGFNAAMVVRAPHARD
selected fasta
>polyketide synthase chain length factor subunit [ChaB protein]
ATGAGTGCCAAGGTCGTGGTCACCGGGCTCGGTGTCGCCGCACCCAACGGGCTCGGCACA
CGCGCCTATTGGGAGGCGACGCTGCGCGGCCGCAGCGGGATCGCCCCGCTGAAGCGGTTC
GACGCACGGGGCTACCCCGCCCGGCTCGCCGGCGAGGTACCCGGCTTTGAACCGGCCGAG
CATCTGCCGGGCCGGTTGCTGCCGCAGACGGACCACATGACCAGGATGGCGCTGGTGGCG
GCCGACTGGGCCCTGGCGGACGCGCGGGCCGATCCGGAGCGGCTCGCGGAGTTCTCGATG
GGAGTGGTGACGGCCAGTTCCTCCGGAGGCTTCGAGTTCGGGCAGCGGGAGCTGGAGAAG
CTGTGGAGCAAGGGCAGCCGGTACGTCAGCTCGTACCAGTCCTTCGCGTGGTTCTACGCC
GTCAACACCGGCCAGATCTCCATCCGGAACGGCATGCGCGGCCCGAGCGGCGTCCTGGTG
AGCGACCAGGCGGGCGGGCTGGACGCCGTGGCGCAGGCCCGCCGGCTGGTGCGCGAAGGG
CTCGCACTGGTCGTATCCGGTGGCGTGGACGCAAGCCTGTGTCCCTGGGGCTGGGTCGCT
CAGCTCAGCACCGGCCGGCTCAGCACCGGCGACGACCCACGGTCCGCGTATCTGCCGTTC
AGCGCCGCGGCGCGCGGCTACGTGCCCGGCGAGGGCGGCGCGATCCTCGTGCTGGAGGAG
GAGGGTGCCGCCGCTCGGCGCCGCCCGGGTGCGCCGTACGGCGAGATCGCGGGCTACGGC
GCGACGTTCGACCCGCATCCGGACAGCGGGCGGCCGCCGCGGCTCCGCGACGCCATCGAG
ATCGCGCTCGGCGACGCCGGGCTCGACCCGTCCGACGTGGACGTCCTCTTCGCCGACGCC
GCTGGACTGCCGGACCTGGACCGGGTGGAGGCCGAGGCGATCAGCGACGTCTTCGGGCCG
CGTGGCGTCCCGGTCACCGCCCCCAAGACGATGACCGGACGGCTCTACTCGGGCGGCGCC
GCCCTGGACCTGGCCGCAGCCCTGCTCTCGCTCAACGAGGGTGTCGTCCCGCCGACCGTC
AACGTGGGGCGGCCGGCCCCCGAGTACGCGATCGACCTGGTCGTGCGGCCGAGGCCCGCC
CCCTTGCGTGTCGCGCTGGTGCTCGCACGCGGGCACGGCGGCTTCAACGCCGCGATGGTC
GTCCGCGCTCCCCACGCCCGCGACTGA
CLF4..362
CLF10..1086

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[4-241] 3.2e-57 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[253-362] 7.79999999999999e-26 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[3-258] 6.29999999999998e-52 G3DSA:3.40.47.10 [271-403] 5.10000000000004e-37 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[2-249] 3.39999972437719e-59 SSF53901 [212-404] 5.79999653854781e-47 SSF53901
SSF53901   Thiolase-like
SignalP
[1-19] 0.726 Signal
Eukaryota   
TMHMM No significant hit
Chart_00100 Chart_00100   Chart_00120 Chart_00120
Page top