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Chart_00190 : CDS information

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Organism
StrainHKI-249
Entry nameChartreusin
Contig
Start / Stop / Direction18,082 / 17,690 / - [in whole cluster]
18,082 / 17,690 / - [in contig]
Locationcomplement(17690..18082) [in whole cluster]
complement(17690..18082) [in contig]
TypeCDS
Length393 bp (130 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.4 hypothetical protein
Producthypothetical protein
Product (GenBank)ChaP protein
Gene
Gene (GenBank)chaP
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[15911378] Biosynthesis of the antitumor agent chartreusin involves the oxidative rearrangement of an anthracyclic polyketide. (Chem Biol. , 2005)
comment
chartreusin生合成clusterの解析論文。
異種性にclusterを発現してchartreusin産生を確認。

ChaP: Dioxygenase

配列解析に基づき、機能予測している。
中間体のdiketone部分を破壊してCO2を失う反応が提唱されている。
この反応に続きlactoneが形成され、aglyconeであるchartarinとなるとされている。

close this sectionSequence

selected fasta
>hypothetical protein [ChaP protein]
MAVELNHTIVLVKDKDASATFMADLLGLPKPKEMGPFAVLQLANDVSIDFMDFRGEGDIV
PGHCAFLISDEEFDQIFGRIREGGIEHWADQYHREPGRINDRDGGRGVYFEDPSGHNMEI
MTRPYGSGGA
selected fasta
>hypothetical protein [ChaP protein]
ATGGCCGTGGAACTGAACCACACCATCGTTCTGGTGAAGGACAAGGACGCCTCGGCAACG
TTCATGGCCGACCTTCTCGGCCTGCCGAAGCCGAAGGAGATGGGCCCGTTCGCGGTACTG
CAACTCGCCAACGACGTGTCGATCGACTTCATGGACTTCCGGGGCGAGGGTGACATAGTT
CCGGGACACTGCGCATTCCTGATCAGCGACGAGGAGTTCGACCAGATCTTCGGACGCATT
CGCGAGGGCGGCATCGAGCACTGGGCGGACCAGTACCACCGGGAGCCCGGCCGGATCAAC
GACCGTGACGGTGGGCGCGGGGTCTACTTCGAGGATCCGAGCGGGCACAACATGGAGATC
ATGACCCGGCCCTACGGCAGCGGCGGGGCGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR018146 Glyoxalase I, conserved site (Conserved_site)
[7-28] PS00934
PS00934   GLYOXALASE_I_1
IPR025870 Glyoxalase-like domain (Domain)
[12-121] 3.8e-10 PF12681
PF12681   Glyoxalase_2
SignalP No significant hit
TMHMM No significant hit
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