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Chart_00280 : CDS information

close this sectionLocation

Organism
StrainHKI-249
Entry nameChartreusin
Contig
Start / Stop / Direction27,621 / 28,787 / + [in whole cluster]
8,041 / 9,207 / + [in contig]
Location27621..28787 [in whole cluster]
8041..9207 [in contig]
TypeCDS
Length1,167 bp (388 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative acyl-CoA dehydrogenase
Product (GenBank)ChaO protein
Gene
Gene (GenBank)chaO
EC number1.3.99.-
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[15911378] Biosynthesis of the antitumor agent chartreusin involves the oxidative rearrangement of an anthracyclic polyketide. (Chem Biol. , 2005)
comment
chartreusin生合成clusterの解析論文。
異種性にclusterを発現してchartreusin産生を確認。

ChaO: Isovaleryl-CoA dehydrogenase

配列解析に基づき、機能予測している。Frankia sp.由来isovaleryl-CoA dehydrogenaseにとても似ているが、chartreusin生合成におけるその機能は不明。
Related Reference
ACC
Q937T2
PmId
[11571189] Cloning and genetic characterization of dca genes required for beta-oxidation of straight-chain dicarboxylic acids in Acinetobacter sp. strain ADP1. (Appl Environ Microbiol. , 2001)
comment
Blast id41%
Acinetobacter sp._dcaA
Acyl-CoA dehydrogenase (EC 1.3.99.-)

beta酸化のstepに関連した3つの遺伝子のノックアウトは、Acinetobacter sp. strain ADP1染色体に導入された。mutantそれぞれはadipate(アジピン酸)で育つことができなかった。mutantsは付加的に飽和させられた直鎖ジカルボン酸を利用するための能力において影響されるので、新しく発見された10kbpDNAはdca(ジカルボン酸dicarboxylic acid)領域と名づけられた。

dcaA(acyl-CoA DH homolog)
dcaE(enoyl-CoA hydratase homolog)
dcaH(hydroxyacyl-CoA DH homolog)

close this sectionSequence

selected fasta
>putative acyl-CoA dehydrogenase [ChaO protein]
MSSLDLLSEDERFVVRTVRDFVDRDVKPVVRELERTDTYPEALIERMKELGVFGLAVPQE
YGGTPVSMPCYVLVTEELARGWMSLAGAMGGHTVVAKLLLHFGTEEQRQRHLPLMATGET
RATMALTETGGGSDLQAMRTVARREGDGYVVNGAKTWITNSRRSRLIALLCKTDPDAEPA
HSGMSVLLVEHGPGLTVSRDLPKLGYKGVESCELSFENHRARADALLGGVEGHGFAQMMR
GLETGRLQVAARALGVGRAALEDALAYAQERESFGKPIWQHQSIGNYLADMATSLTAARQ
LTLYAARRAEAGHRVDMEAGMAKLFASETAMQIALNAVRIHGGYGYSTEFDVERYFRDAP
LMIVGEGTNEIQRNIIADRLVKRGGLDV
selected fasta
>putative acyl-CoA dehydrogenase [ChaO protein]
ATGAGCAGTCTCGACCTCCTGTCCGAGGACGAGCGGTTCGTCGTCAGGACGGTGCGCGAC
TTCGTGGACAGGGACGTCAAGCCCGTCGTCCGGGAGCTGGAGCGCACCGACACATACCCC
GAAGCCCTCATCGAGCGGATGAAGGAACTCGGCGTCTTCGGACTCGCCGTCCCGCAGGAG
TACGGCGGCACACCCGTCTCCATGCCCTGCTACGTCCTCGTCACCGAGGAGCTGGCCCGG
GGCTGGATGAGCCTGGCCGGCGCGATGGGCGGGCACACCGTCGTCGCCAAGCTGCTGCTG
CACTTCGGCACCGAGGAGCAGCGGCAGCGCCACCTGCCCCTGATGGCCACCGGCGAGACC
CGCGCGACGATGGCACTGACCGAGACCGGCGGCGGCTCCGACCTCCAGGCGATGCGCACC
GTCGCCCGAAGGGAGGGCGACGGCTATGTGGTGAACGGCGCGAAGACGTGGATCACCAAC
TCCCGCCGCTCGCGGCTCATCGCCCTGCTGTGCAAGACCGACCCGGACGCCGAGCCCGCG
CACAGCGGCATGTCCGTCCTCCTCGTCGAGCACGGACCGGGCCTGACCGTCTCCCGTGAT
CTGCCCAAGCTCGGCTACAAGGGCGTGGAGAGCTGCGAGCTGTCCTTCGAGAACCACCGC
GCACGTGCCGACGCGCTGCTGGGGGGAGTGGAGGGGCACGGCTTCGCCCAGATGATGCGG
GGTCTGGAGACCGGCCGCCTCCAAGTCGCCGCCCGCGCCCTGGGCGTGGGCCGGGCCGCC
CTGGAGGACGCGCTCGCCTACGCGCAGGAACGCGAGTCCTTCGGCAAGCCGATCTGGCAG
CACCAGTCGATCGGCAACTACCTTGCCGACATGGCGACTTCGCTCACCGCCGCACGCCAG
CTCACCCTCTATGCCGCCCGTCGGGCGGAGGCCGGACACCGGGTGGACATGGAGGCCGGC
ATGGCGAAGCTCTTCGCCTCCGAGACGGCCATGCAGATCGCTCTCAACGCCGTCCGCATC
CATGGCGGCTACGGCTACTCGACCGAGTTCGACGTAGAGCGCTACTTCCGGGACGCGCCG
CTGATGATCGTCGGAGAGGGCACCAACGAGATCCAGCGCAACATCATCGCCGACCGGCTC
GTGAAGCGGGGAGGACTGGACGTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006089 Acyl-CoA dehydrogenase, conserved site (Conserved_site)
[125-137] PS00072
PS00072   ACYL_COA_DH_1
IPR006090 Acyl-CoA oxidase/dehydrogenase, type 1 (Domain)
[232-380] 2.19999999999997e-47 PF00441
PF00441   Acyl-CoA_dh_1
IPR006091 Acyl-CoA oxidase/dehydrogenase, central domain (Domain)
[123-219] 4.9e-32 G3DSA:2.40.110.10
G3DSA:2.40.110.10   Acyl_CoA_DH/ox_M
[123-174] 5.5e-19 PF02770
PF02770   Acyl-CoA_dh_M
IPR006092 Acyl-CoA dehydrogenase, N-terminal (Domain)
[8-119] 5.30000000000001e-30 PF02771
PF02771   Acyl-CoA_dh_N
IPR009075 Acyl-CoA dehydrogenase/oxidase C-terminal (Domain)
[232-382] 3.89999861795218e-51 SSF47203
SSF47203   AcylCoADH_C_like
[220-384] 1.99999999999999e-59 G3DSA:1.20.140.10
G3DSA:1.20.140.10   AcylCoA_DH_1/2_C
IPR009100 Acyl-CoA dehydrogenase/oxidase (Domain)
[1-231] 2.39999798157263e-70 SSF56645
SSF56645   AcylCoA_dehyd_NM
IPR013786 Acyl-CoA dehydrogenase/oxidase, N-terminal (Domain)
[7-120] 7.5e-37 G3DSA:1.10.540.10
G3DSA:1.10.540.10   AcylCoA_DH/ox_N
SignalP No significant hit
TMHMM No significant hit
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