Chart_00320 : CDS information

Location

Organism	Streptomyces chartreusis
Strain	HKI-249
Entry name	Chartreusin
Contig	AJ786383
Start / Stop / Direction	31,652 / 32,521 / + [in whole cluster] 12,072 / 12,941 / + [in contig]
Location	31652..32521 [in whole cluster] 12072..12941 [in contig]
Type	CDS
Length	870 bp (289 aa)

Annotation

Category	4.3 transport
Product	putative ABC transporter permease protein
Product (GenBank)	ChaT4 protein
Gene
Gene (GenBank)	chaT4
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q4R0K0 PmId [15911378] Biosynthesis of the antitumor agent chartreusin involves the oxidative rearrangement of an anthracyclic polyketide. (Chem Biol. , 2005) comment chartreusin生合成clusterの解析論文。異種性にclusterを発現してchartreusin産生を確認。 ChaT4: ABC-type Mn2+/Zn2+ transport systems, permease components 配列解析に基づき、機能予測している。 ChaT2-T5はKineococcus radiotolerans由来ABC ion transporters componentsに似ている。
Related Reference	ACC Q9RNI8 PmId [10564500] ( , ) comment 355th(Q9RNI8) 30%, 6e-17 Streptococcus pyogenes_mtsC Integral membrane protein MtsC Streptococcus pyogenesで、LraI (lipoprotein receptor-associated antigen I) familyのABC transporterを調べた。mtsABC operonを欠く変異体は、野生型に比べてFeやZnの取り込みが減少した。MtsAのFe, Zn, Mn, Cuとの結合を調べた。 mts = metal transporter of Streptococcus mtsA (lipoprotein) mtsB (ATP-binding protein) mtsC (integral membrane protein)

Sequence

>putative ABC transporter permease protein [ChaT4 protein]
MTLAQGIWQQIFAFDHYGELLALVRNSLAAGAALGLVGGLVGVFVIMRDLPFAVHGISEL
SFAGASAALLLGVNVVAGSIVGSLVAAGAIGLLGARARDRNSVIGVIMPFGLGLGVLFLA
LYKGRAANKFGLLTGQIVAVDTPQMSWLLGTSAVVLVALAVMWRPLVFASADAEVAEARG
VPVRGMSFAFMIVLGLAVALSVQIVGALLVLTLVVTPAAAAARVTASPVLLPVLSAVFAV
ASIEGGILLALGSSIPISPYVTTISFGIYLVCRRFGGKRARTGGVGVQG

>putative ABC transporter permease protein [ChaT4 protein]
ATGACCCTCGCCCAGGGAATCTGGCAGCAGATCTTCGCCTTCGACCACTACGGCGAACTG
CTCGCCCTCGTCCGGAACTCGCTCGCCGCCGGGGCCGCGCTCGGGCTGGTCGGCGGCCTG
GTGGGCGTGTTCGTGATCATGCGGGACCTGCCGTTCGCGGTGCACGGGATCAGCGAGCTG
TCGTTCGCCGGGGCCTCGGCCGCGCTGCTGCTGGGCGTGAACGTCGTGGCCGGGTCGATC
GTCGGCTCCCTCGTCGCGGCGGGGGCCATCGGGCTGCTCGGGGCGCGGGCCCGGGACCGC
AACTCGGTGATCGGCGTCATCATGCCGTTCGGCCTCGGGCTCGGCGTGCTGTTCCTCGCG
CTGTACAAGGGCCGCGCGGCGAACAAGTTCGGCCTGTTGACCGGGCAGATCGTCGCCGTC
GACACCCCGCAGATGTCCTGGCTGCTCGGCACGTCGGCGGTGGTGCTGGTCGCGCTCGCG
GTGATGTGGCGACCGCTCGTCTTCGCCAGTGCCGACGCGGAGGTGGCCGAGGCGCGGGGC
GTGCCGGTGCGGGGAATGTCCTTCGCCTTCATGATCGTGCTGGGCCTCGCGGTCGCGCTG
TCCGTCCAGATCGTCGGCGCCCTTCTGGTCCTGACCCTCGTCGTCACACCCGCGGCGGCC
GCGGCTCGCGTCACGGCCTCGCCGGTGCTGCTTCCCGTCCTGAGCGCGGTGTTCGCGGTG
GCCTCCATCGAGGGCGGCATCCTGCTGGCCCTCGGCAGCAGCATCCCCATCAGCCCGTAC
GTCACCACGATCTCGTTCGGCATCTACCTCGTGTGCAGGCGTTTCGGCGGCAAACGGGCC
CGGACCGGTGGGGTCGGCGTGCAGGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001626 ABC-3 (Family) PF00950 ABC-3
SignalP	Eukaryota Bacteria, Gram-positive
TMHMM	Transmembrane 8

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