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Chart_00330 : CDS information

close this sectionLocation

Organism
StrainHKI-249
Entry nameChartreusin
Contig
Start / Stop / Direction32,568 / 34,631 / + [in whole cluster]
12,988 / 15,051 / + [in contig]
Location32568..34631 [in whole cluster]
12988..15051 [in contig]
TypeCDS
Length2,064 bp (687 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative cation-transporting ATPase
Product (GenBank)ChaT5 protein
Gene
Gene (GenBank)chaT5
EC number3.6.3.-
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[15911378] Biosynthesis of the antitumor agent chartreusin involves the oxidative rearrangement of an anthracyclic polyketide. (Chem Biol. , 2005)
comment
chartreusin生合成clusterの解析論文。
異種性にclusterを発現してchartreusin産生を確認。

ChaT5: Putative cation-transporting ATPase

配列解析に基づき、機能予測している。
ChaT2-T5はKineococcus radiotolerans由来ABC ion transporters componentsに似ている。
ChaT5は、S.coelicolorのion-transporting ATPaseに高い類似性を持つ。
Related Reference
ACC
O31688
PmId
comment
Blast 132nd, id39%, 1e-115
B.subtilis_zosA(ykvW)
Zinc-transporting ATPase(EC 3.6.3.5)
(Zn(2+)-translocating P-type ATPase)

hydrogen peroxide(H2O2)によって誘発される、PerR regulonのひとつとして同定。
zosAの発現はH2O2によって誘導され、PerR metalloregulatory proteinによって抑制される。

zosA mutantは、
・Cd, Ni, Co, Cu, Fe, Mn ions耐性には影響なし
・Zn(II) sensitivity増
・cell内Zn濃度減
であることから、ZosAはZn(II) の取り込みを行うことが示されている。

また、ZosAはH2O2, thiol-oxidizing agent diamide耐性にも関与。
細胞内Zn(II)増加がthiolsを酸化から保護するかもしれない。

zos = "z"inc-uptake system induced by "o"xidative "s"tress

close this sectionSequence

selected fasta
>putative cation-transporting ATPase [ChaT5 protein]
MPATLTPPAADPAASVRAGAVPRRRTRLLALPEVRRAAAATALFLLALPLQLAGAPALIW
GALYVLSYATGGWEPGWEGLKALKGRTLDVDLLMVVAALGAASIGQVMDGALLIVIFATS
GALEALATARTADSVRGLLDLAPATATRIAADGTEQTLPVDELAVGDTVLVRPGERVGAD
GRVLDGASEVDQTSITGEPLPALKEPGDEVFAGTLNGTGALRVRVERDASDSVIARIVRM
VEEASETKAPTQLFIEKVEQRYSLGMVAATLAVFGVPLAFGEAFSEALLRGMTFMIVASP
CAVVLATMPPLLSAIANAGRHGVLVKSAVAMERLGQIDAVALDKTGTLTEGTPRVVDVRP
LRERGLDEVELLTFAAAAEHPSEHPLARAVVDAARERGLDLPATEGFTSAPGIGVMAVVG
GRTVQVGAPARLLAEAEAEVEVEVEAKCAADAGGDTDGVVAALVRELEESGRSAVLVVVD
GTPAGVLGIADRLRPDAAATVAALTVLTGTAPTLLTGDNPRAAARLAAEAGVEDVRAGLL
PQEKVGAVRELEGAGRRVMVVGDGVNDAPALAAAHIGVAMGRAGSDLALETADAVIVRDE
LVAVPATVALSRRARELVVQNLVIAGVFIAGLVVWDLAGTLPLPLGVAGHEGSTVLVGLN
GLRLLRDSAWKRAAPEGTGTPAGAAKL
selected fasta
>putative cation-transporting ATPase [ChaT5 protein]
ATGCCTGCCACGCTCACCCCACCCGCGGCCGACCCCGCCGCCTCCGTGCGCGCCGGAGCC
GTCCCCAGGCGGCGCACCCGGCTGCTCGCGCTGCCGGAGGTCCGCCGGGCCGCCGCGGCC
ACCGCGCTGTTCCTGCTCGCGCTGCCGCTCCAGCTCGCCGGAGCGCCCGCCTTGATCTGG
GGTGCGCTGTACGTGCTGTCGTACGCCACCGGCGGCTGGGAGCCGGGCTGGGAAGGGCTC
AAGGCGCTCAAGGGCAGGACCCTCGACGTCGATCTGCTGATGGTCGTCGCCGCGCTCGGC
GCCGCATCGATCGGGCAGGTCATGGACGGCGCCCTGCTGATCGTCATCTTCGCGACCTCC
GGCGCGCTGGAGGCGCTCGCCACCGCCCGCACCGCCGACTCGGTGCGCGGCCTGCTCGAC
CTGGCGCCCGCCACGGCCACCCGCATCGCCGCCGACGGCACCGAACAAACCCTCCCCGTC
GACGAGTTGGCGGTCGGCGACACCGTCCTCGTACGGCCCGGCGAGCGCGTCGGGGCCGAC
GGGCGGGTGCTCGACGGAGCGAGCGAGGTGGATCAGACGAGCATCACCGGCGAGCCGCTC
CCGGCGTTGAAGGAGCCGGGCGACGAAGTCTTCGCGGGCACGCTCAACGGCACCGGCGCG
CTGCGCGTCCGGGTCGAACGGGACGCCTCCGACTCCGTGATCGCCCGCATCGTACGGATG
GTGGAGGAGGCGTCGGAGACCAAGGCGCCCACGCAGCTGTTCATCGAGAAGGTCGAACAG
CGTTACTCGCTGGGCATGGTGGCCGCGACGCTCGCCGTGTTCGGTGTGCCGCTCGCGTTC
GGTGAGGCGTTCTCCGAGGCGCTGCTGCGTGGGATGACCTTCATGATCGTGGCCTCGCCG
TGCGCGGTGGTCCTGGCCACCATGCCACCCCTGCTGTCGGCCATCGCCAACGCCGGCCGG
CACGGCGTGCTGGTGAAGTCGGCCGTGGCGATGGAACGCCTCGGCCAGATCGACGCCGTG
GCGCTGGACAAGACCGGCACGCTCACGGAGGGGACGCCACGGGTCGTCGACGTACGGCCG
CTGCGTGAACGTGGCCTGGACGAGGTCGAGTTGCTGACGTTCGCCGCGGCGGCCGAGCAC
CCCAGCGAGCACCCGCTGGCCCGCGCGGTGGTGGACGCGGCACGCGAGCGTGGCCTCGAC
CTGCCGGCCACCGAGGGCTTCACATCCGCGCCCGGGATCGGCGTCATGGCCGTCGTCGGG
GGCCGTACGGTCCAGGTGGGCGCCCCGGCGCGGCTGCTCGCCGAAGCCGAAGCCGAGGTA
GAGGTTGAAGTCGAGGCCAAGTGCGCAGCCGATGCCGGCGGCGACACCGACGGCGTGGTC
GCGGCACTGGTCCGGGAACTGGAGGAGTCCGGCCGTAGCGCCGTCCTCGTGGTCGTCGAC
GGAACCCCGGCCGGTGTGCTCGGCATCGCCGACCGGCTGCGTCCCGACGCGGCCGCCACG
GTCGCCGCTCTGACCGTCCTCACCGGCACCGCCCCGACCCTCCTCACCGGCGACAACCCC
CGTGCCGCCGCGCGCCTGGCAGCCGAGGCCGGTGTCGAGGACGTGCGGGCCGGGCTGCTG
CCGCAGGAAAAGGTGGGCGCGGTGCGGGAGTTGGAGGGTGCGGGGCGCCGGGTGATGGTC
GTCGGGGACGGCGTCAACGACGCGCCCGCGCTGGCCGCCGCGCACATCGGCGTCGCCATG
GGCCGAGCCGGCTCCGACCTCGCCCTGGAGACCGCCGACGCGGTGATCGTGCGCGACGAG
CTCGTCGCCGTACCCGCCACCGTCGCCCTGTCCCGCCGCGCCCGCGAGCTGGTCGTGCAG
AACCTCGTGATCGCCGGGGTGTTCATCGCCGGCCTGGTCGTCTGGGACCTGGCCGGGACG
CTGCCGCTGCCTCTCGGGGTCGCGGGCCACGAGGGCTCCACGGTCCTCGTCGGCCTGAAC
GGACTGCGCCTGCTGCGGGACTCGGCCTGGAAGCGGGCGGCGCCGGAGGGAACCGGGACC
CCGGCGGGTGCCGCAAAGTTGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001366 ATPase, P-type, cadmium-transporting (Family)
[54-71] 1.79999951654677e-08 PR00941 [125-145] 1.79999951654677e-08 PR00941 [207-223] 1.79999951654677e-08 PR00941 [226-240] 1.79999951654677e-08 PR00941
PR00941   CDATPASE
IPR001757 ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter (Family)
[191-205] 9.30000048475975e-24 PR00119 [341-355] 9.30000048475975e-24 PR00119 [485-496] 9.30000048475975e-24 PR00119 [508-518] 9.30000048475975e-24 PR00119 [561-580] 9.30000048475975e-24 PR00119 [585-597] 9.30000048475975e-24 PR00119
PR00119   CATATPASE
[114-376] 5.19999999999996e-37 TIGR01494 [464-625] 1.8e-28 TIGR01494
TIGR01494   ATPase_P-type
IPR005834 Haloacid dehalogenase-like hydrolase (Domain)
[337-574] 1.5e-37 PF00702
PF00702   Hydrolase
IPR006416 ATPase, P-type, heavy metal translocating (Family)
[90-664] TIGR01525
TIGR01525   ATPase-IB_hvy
IPR008250 ATPase, P-type, ATPase-associated domain (Domain)
[113-332] 1.40000000000001e-46 PF00122
PF00122   E1-E2_ATPase
IPR018303 ATPase, P-type phosphorylation site (PTM)
[343-349] PS00154
PS00154   ATPASE_E1_E2
IPR023214 HAD-like domain (Domain)
[337-612] 2.69998997787809e-41 SSF56784
SSF56784   HAD-like_dom
[331-355] 1.9e-34 G3DSA:3.40.50.1000 [491-625] 1.9e-34 G3DSA:3.40.50.1000
G3DSA:3.40.50.1000   HAD-like_dom
IPR023299 ATPase, P-type, cytoplasmic domain N (Domain)
[356-490] 3.90000000000001e-24 G3DSA:3.40.1110.10
G3DSA:3.40.1110.10   ATPase_P-typ_cyto_domN
IPR023300 ATPase, P-type, cytoplasmic transduction domain A (Domain)
[92-242] 2.80000000000001e-23 G3DSA:2.70.150.10
G3DSA:2.70.150.10   ATPase_P-typ_cyto_domA
SignalP
[1-57] 0.974 Signal
Eukaryota   
[1-39] 0.432 Signal
Bacteria, Gram-positive   
TMHMM
[42-64] Transmembrane (i-o) [95-117] Transmembrane (o-i) [262-284] Transmembrane (i-o) [294-316] Transmembrane (o-i) [617-639] Transmembrane (i-o)
Transmembrane 5   
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