Chth_00150 : CDS information

Location

Organism	Streptomyces antibioticus
Strain	DSM 40725
Entry name	Chlorothricin
Contig	DQ116941
Start / Stop / Direction	19,014 / 17,698 / - [in whole cluster] 19,014 / 17,698 / - [in contig]
Location	complement(17698..19014) [in whole cluster] complement(17698..19014) [in contig]
Type	CDS
Length	1,317 bp (438 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose 2,3-dehydratase
Product (GenBank)	ChlC3
Gene
Gene (GenBank)	chlC3
EC number
Keyword	D-olivose
Note
Note (GenBank)	2,3-dehydratase
Reference	ACC Q0R4N9 PmId [16793515] Genetic characterization of the chlorothricin gene cluster as a model for spirotetronate antibiotic biosynthesis. (Chem Biol. , 2006) comment chlorothricin(CHL) gene clusterに関する文献。 chlC3(438 aa): 2,3-dehydratase chlC3自体の機能実験は行われていないが、相同性よりdNDP-hexose dehydrataseと予想されており、2,3-dehydratationを行って、3-ketoreductaseをエンコードしていると予想されているchlC4と共にalpha, beta-unsaturated 4-ketosugarを形成するとされている。
Related Reference	ACC Q9ALN6 NITE Spino_00070 PmId [18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008) comment <Probable NDP-hexose-2,3-dehydratase(spnO), Saccharopolyspora spinosa, BLAST 21; id=49%> Forosamine合成の初期段階でNDP-4-keto-6-deoxy-glucoseの2,3位の脱水反応を行う。使用している株はNRRL 18537. dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase_SpnO dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), and either NADH or NADPHと一緒にSpnO and SpnNをインキュベートし、NAD(P)Hの消費量をフォローしてtandem reactionを測定。SpnO or SpnNのどちらかを欠いた場合、消費が見られなかった。 dTDP-3,4-diketo-2,6-dideoxy-D-glucoseは不安定で急速にdTDP and maltolに分解してしまうため、SpnOのみの触媒効率は定量できていない。 -- SpnNはdTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductaseであることが他で報告されている。

Sequence

>putative NDP-hexose 2,3-dehydratase [ChlC3]
MTEAAVDTPFRAWWREQASAQRSEVARIPFAELDGWGFEPHGGNLVHASGKFFTVEGLRI
EPDDGREARQPIIHQPEIGVLGILVKEFDGVVHCLMQAKMEPGNLNTVQLSPTVQATRSN
YLRVHRGGRTRYLEHFWGAGRGRVLVDVLQSEQGAWFWHKRNRNMIVQVDGDVPVHEAFR
WVALPELLALLHSDNLVNMDARTVLGCLPRDLLPRQGRTGAFAAALRCSYETGADAGAAR
SWLLDVKSRGRWTTRLIPLNDTHGWIRKSDEISADDGSGFRIIAVRVRSRSREVPEWTQP
LLAPRDEGLAAFLVRRAGGTLQVLVQARYEPGLLEHVELAPTVRTPPVTADEEVPYLKDV
LSADHRNIRFDALLSEEGGRFHHALTRYQVIEVADGQTLEVGDNHRWMTIGQLMELVRHG
HYLNIEARSLLACLHSLR

>putative NDP-hexose 2,3-dehydratase [ChlC3]
GTGACTGAAGCAGCAGTGGATACCCCCTTCCGCGCCTGGTGGCGGGAGCAAGCGTCGGCC
CAGCGGTCGGAGGTGGCACGCATCCCGTTCGCGGAGCTCGACGGCTGGGGCTTCGAACCG
CACGGCGGGAACCTGGTGCACGCGAGCGGGAAGTTCTTCACCGTCGAGGGCCTGCGCATA
GAACCCGACGATGGACGGGAAGCCCGCCAGCCGATCATCCATCAGCCCGAGATCGGCGTT
CTCGGTATCCTGGTCAAGGAGTTCGACGGGGTCGTGCACTGCCTGATGCAGGCCAAGATG
GAGCCCGGAAACCTCAACACCGTCCAGCTCTCGCCGACGGTCCAGGCGACCCGCAGCAAC
TACCTGCGGGTACACCGCGGCGGCCGCACCCGCTATCTGGAGCACTTCTGGGGAGCAGGG
CGCGGCAGGGTGCTCGTGGACGTCCTGCAGTCCGAACAGGGCGCCTGGTTCTGGCACAAA
CGCAACCGCAACATGATCGTCCAAGTGGACGGCGATGTGCCCGTGCACGAGGCCTTCCGC
TGGGTGGCCCTGCCCGAGCTGCTGGCCCTGCTGCACAGCGACAACCTCGTCAACATGGAC
GCCCGCACCGTACTGGGCTGCCTGCCACGGGACTTGCTGCCCCGCCAGGGCCGTACGGGC
GCCTTCGCCGCGGCGCTGCGCTGCTCGTACGAGACCGGTGCCGACGCCGGGGCCGCGCGC
AGCTGGCTGCTCGATGTGAAAAGCCGCGGCCGCTGGACGACCCGGCTGATCCCGCTGAAC
GACACCCACGGCTGGATCCGGAAAAGCGACGAGATCAGCGCCGACGACGGCTCGGGATTC
CGGATCATCGCGGTCCGGGTGCGCTCCCGCAGCCGTGAAGTCCCCGAATGGACCCAGCCG
TTGCTGGCCCCCCGGGACGAGGGTCTGGCCGCCTTCCTGGTCCGGCGCGCCGGCGGCACC
CTGCAGGTGCTGGTCCAGGCGCGCTACGAGCCCGGCCTGCTGGAGCACGTGGAGCTGGCG
CCCACGGTCCGCACCCCACCGGTCACCGCCGACGAGGAGGTGCCCTACCTGAAGGACGTG
CTGTCCGCCGACCACCGCAACATCCGCTTCGACGCCCTGCTGTCCGAGGAGGGCGGGCGC
TTCCACCATGCGCTCACGCGCTACCAGGTGATCGAGGTGGCGGACGGGCAGACGCTCGAA
GTCGGCGACAACCACCGGTGGATGACGATCGGTCAGCTCATGGAACTGGTGCGGCACGGC
CACTACCTGAACATCGAGGCCCGCAGCCTGCTCGCCTGCCTGCACAGCCTGCGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005212 NDP-hexose 2,3-dehydratase (Domain) PF03559 Hexose_dehydrat
SignalP	No significant hit
TMHMM	No significant hit

Chth_00140 Chth_00160