Chth_00290 Chth_00290   Chth_00310 Chth_00310

Chth_00300 : CDS information

close this sectionLocation

Organism
StrainDSM 40725
Entry nameChlorothricin
Contig
Start / Stop / Direction76,074 / 81,635 / + [in whole cluster]
76,074 / 81,635 / + [in contig]
Location76074..81635 [in whole cluster]
76074..81635 [in contig]
TypeCDS
Length5,562 bp (1,853 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)ChlA4
Gene
Gene (GenBank)chlA4
EC number
Keyword
Note
Note (GenBank)
  • type I PKS
Reference
ACC
PmId
[16793515] Genetic characterization of the chlorothricin gene cluster as a model for spirotetronate antibiotic biosynthesis. (Chem Biol. , 2006)
comment
chlorothricin(CHL) gene clusterに関する文献。

chlA4(1,853 aa): typeI PKS: KS-AT-dh-KR-ACP
chlorothricin(CHL) を合成するPKSをコードする。

ChlA4のmodule 8にあるDH domainは、conserved motifs内のE, D等の酸性アミノ酸残基がQ, G等の中性アミノ酸残基で置換されているのでinactiveであると考えられる。産物構造的にも必要とされない。

close this sectionPKS/NRPS Module

A8 malonyl-CoA
KS36..413
AT566..880
dh929..1093
KR1416..1596
ACP1695..1765

close this sectionSequence

selected fasta
>polyketide synthase [ChlA4]
MTVTPPEKIVEALRASLLENERLRQENQRSADKAREPIAIVGMACRYPGGVASPEQLWQL
VAEGKDAITTFPSTRGWDLGGIYHPDPDHPGTSYSRHGGFLHDAHEFDADFFGMSPREAL
ATDPQQRLLLEAAWETLERAGLDPHSLRGSRTGVFTGIMYGDYGSRLHHHVPQDFEGQIG
IGSSASVASGRVAYTLGLEGPAVTIDTACSSSLVALHLAAQALRNGECDLALAGGVTVMA
TPSTFIEFSRQRGLAPDGRCKPFAATADGTGWSEGIGLLLMERLTDAQQNGHPILAHLRG
SAVNQDGASSQLTAPNGPSQQRVIRAALDNARLTPDQVDVVEAHGTGTGLGDPIEAQALL
AAYGRDRAPDRPLWLGSIKSNIGHAQAAAGVAGIIKMVEAMRHGVLPQTLHVDEPTPHVD
WSAGAVSLLTETRPWPETGRPRRAAVSSFGISGTNAHVILEQAPEADEAPSETTDGPLAW
VLSAKTEAALRVQAAHLRDLVTRDAELPLADIGFSLATTRARLEQRAAVVAEDRAEFLAG
LDALAAGADHDQVVRGSSVASGRTAFLFAGQGSQRPGMGRELYDSQPVFAQALDEVCALL
DPHLDVPLREVMWAEEGTEQAALLEDTLYTQPALFALETALFRLLQHLGVTPHYLVGHSI
GEITAAHAAGILTLDDACTLVAARARLLHGLPTGGTMTALQATEAEVEEALQDHPGVTIA
ALNTPHSTVISGDTDAVTHLAALFAEQGRKVTPLHVSHAFHSPHLDPVLDDFHTVAATLT
YHQPRIPIISTLTGEPAGTGDLTTPDYWTRQLRETVRFHPALTTLTELNTTTHLELGPDT
TLTTLTHATTDTTAIPLLHPHKPETHTLLTALATAHTHGTPLDWTTIFAPHHPSRIPLPT
YPFQHRPYWLHHTPTLTNATDLGQTPTPHPLLAATAQLSNGAHLFTGRLSLATHPWLAHH
TIRGTVVLPAAALVDLALHASHHTGHGGIGDLTLHTPLALAEGAAVRLRITVETANGDAP
ATLSIDSQPESDPESAWTTHASATLGLSPAEGVNEQPLSVNWPPREARSRAVDDLYEDLT
DVGLAYAPAFQGVRALWQHGDTLYVEVGLPEEPTLPDTAWDGFGVHPALLDAALHPLLRA
HGDGGRTTLRWSGISLHAEPSPVLRVRLTPTADDTFAVEIADDTGALVLTAGAVRLGPLP
DSLPRTGRHVAPGDALFRVGWKPAAPESREFAPGRWGLLGEDLLGVRPALEAAGQVGRIG
SYPDMKALLSVLDDVVPQSVIAPCVSPEADDVAQAVHTLTERVLSLLQSWLAEPRLTASR
LVVVTRGAVAASDTEKPDDLGAASVWGLLRSAQSENPDQFQLIDTDGTAASLTALATVLT
SGEPQVALREGKMLTPHLTKEPVAKDAVAPALDPEGTVLITGGTGTLGALTARHLITQHG
ARRLLLASRRGPEADGATELHDGLTELGAHVTITACDTADPAQLADLLTTIPTEHPLTAV
IHTAGITHDATLTTLTPEQLHTVLKPKVDAAWHLHHQTQHLHLTAFILYSSAAGILGNPG
QANYAAANTFLDTLAHHRHTQGLPATSLAWGLWTQTSTITATLDKDRGISSAGVTALPTD
QALALFDAALASGRPQLTPIGLDLRSLRAAAEAGVLPAPLRTLVERSGPRRRGRTEPSLA
RRLAGLSETEQSALLLELVRTHTAAVLGHASAEAIATQRAFHELGFDSLAAVKLRNQLNT
ATGLRLPMTVIFDHPHVAALADRLHTELRTEGTAPGAALAELERFEATVLSLAPDSDQRS
GLAARLRALLWKLDADPHTATRQAAPGDDLGSASDDELFSVLDNELGIARTHD
selected fasta
>polyketide synthase [ChlA4]
ATGACGGTGACACCCCCCGAAAAGATCGTCGAGGCGCTGCGAGCGTCGCTGCTGGAGAAC
GAACGGCTTCGGCAGGAGAACCAGCGGTCGGCGGACAAGGCCCGCGAGCCGATCGCCATC
GTGGGCATGGCCTGCCGTTACCCGGGCGGCGTGGCATCCCCCGAACAGCTCTGGCAACTG
GTCGCCGAGGGCAAGGACGCCATCACGACCTTCCCCAGCACCCGTGGCTGGGACCTCGGC
GGGATCTACCACCCCGACCCCGACCACCCGGGGACCAGTTACTCCCGCCACGGCGGCTTT
CTCCACGACGCGCACGAGTTCGACGCCGACTTCTTCGGCATGAGCCCCCGCGAGGCCCTG
GCCACGGACCCACAGCAGCGCCTGCTGCTGGAGGCCGCGTGGGAAACGCTGGAGCGCGCC
GGCCTCGACCCGCACTCCCTGCGCGGCAGCCGCACCGGAGTCTTCACCGGCATCATGTAC
GGCGACTACGGCAGCCGTCTCCACCACCACGTTCCGCAGGACTTCGAAGGACAGATCGGC
ATCGGAAGTTCGGCGAGCGTGGCCTCCGGCCGCGTCGCCTACACCCTGGGCCTGGAAGGC
CCCGCCGTCACCATCGACACCGCCTGCTCCTCCTCCCTGGTCGCCCTGCACCTGGCAGCC
CAGGCCCTCCGCAACGGCGAATGCGACCTCGCCCTCGCCGGCGGCGTCACCGTCATGGCC
ACCCCCTCCACCTTCATCGAGTTCAGCCGCCAACGCGGCCTCGCACCCGACGGCCGCTGC
AAACCCTTCGCCGCAACCGCAGACGGCACCGGCTGGAGCGAAGGCATCGGTCTCCTCCTC
ATGGAACGCCTCACCGACGCCCAACAAAACGGCCATCCCATCCTCGCCCACCTACGCGGC
AGTGCCGTCAACCAGGACGGCGCCAGCAGTCAGCTCACCGCGCCCAACGGCCCGTCGCAG
CAGCGTGTCATCCGCGCGGCGCTGGACAATGCCCGCCTCACACCGGACCAGGTCGACGTG
GTCGAGGCCCATGGCACCGGCACCGGCCTGGGCGACCCGATCGAAGCACAGGCGCTGCTC
GCCGCCTACGGCCGGGACCGCGCCCCGGACCGACCGCTGTGGCTCGGCTCCATCAAGTCG
AACATCGGCCACGCCCAAGCCGCCGCAGGCGTCGCCGGCATCATCAAGATGGTCGAAGCC
ATGCGGCACGGGGTACTGCCCCAGACCCTGCACGTCGACGAACCCACCCCCCACGTCGAC
TGGTCCGCGGGAGCCGTATCCCTCCTCACCGAGACCCGCCCCTGGCCGGAAACGGGACGC
CCGAGGCGCGCCGCCGTGTCCTCTTTCGGTATCAGCGGCACCAACGCCCACGTCATCCTG
GAGCAGGCACCGGAAGCCGACGAGGCTCCGTCCGAGACGACGGACGGGCCGCTGGCATGG
GTGCTCTCCGCCAAGACCGAAGCCGCGCTACGCGTCCAAGCCGCGCACCTACGTGACCTG
GTGACCCGCGACGCCGAACTCCCGCTAGCCGACATCGGTTTCTCCCTGGCCACCACGCGA
GCGCGTCTGGAGCAGCGGGCGGCGGTCGTGGCCGAGGACCGAGCAGAATTCCTCGCCGGC
CTCGACGCCCTGGCAGCCGGCGCCGACCATGACCAAGTCGTCCGGGGAAGCAGCGTCGCA
TCGGGCAGGACGGCGTTCCTGTTCGCCGGGCAGGGCAGTCAGCGCCCGGGCATGGGACGG
GAGCTGTACGACAGCCAGCCCGTCTTCGCCCAGGCCCTCGACGAGGTCTGCGCCCTGCTC
GACCCGCACCTCGACGTACCCCTGCGTGAGGTCATGTGGGCCGAAGAAGGCACCGAGCAG
GCAGCCCTCCTGGAAGACACCCTCTACACCCAGCCCGCGCTGTTCGCACTGGAGACCGCC
CTCTTCCGGCTCCTCCAGCACCTCGGCGTCACACCCCACTACCTCGTCGGGCACTCCATC
GGAGAGATCACCGCAGCCCACGCCGCCGGCATCCTCACCCTCGACGACGCCTGCACCCTG
GTCGCGGCCCGCGCCCGCCTCCTCCACGGCCTGCCCACGGGCGGCACCATGACCGCCCTC
CAAGCCACCGAAGCCGAAGTCGAAGAAGCCCTCCAAGATCACCCCGGCGTCACCATCGCC
GCCCTCAACACCCCCCACAGCACCGTCATCTCCGGCGACACCGACGCCGTCACACACCTC
GCGGCCCTCTTCGCAGAGCAAGGCCGCAAGGTGACACCACTGCACGTCAGCCACGCCTTC
CACTCCCCCCACCTCGACCCCGTCCTCGACGACTTCCACACAGTCGCCGCAACCCTCACC
TACCACCAGCCCCGCATCCCGATCATCTCCACCCTCACCGGAGAACCCGCCGGAACCGGC
GACCTCACCACCCCCGACTACTGGACACGACAGCTCCGCGAAACCGTCCGCTTCCACCCC
GCCCTCACCACACTCACCGAGCTCAACACCACCACCCACCTAGAACTCGGCCCCGACACC
ACCCTCACCACACTCACACACGCGACAACCGACACCACCGCCATCCCTCTCCTCCACCCC
CACAAACCCGAGACCCACACCCTCCTCACCGCCCTCGCCACCGCCCACACCCACGGCACC
CCCCTCGACTGGACCACGATCTTCGCCCCCCACCACCCCAGTCGCATCCCGCTCCCCACC
TACCCCTTCCAGCACCGCCCCTACTGGCTCCACCACACCCCCACCCTCACCAACGCCACC
GACCTCGGCCAAACCCCCACCCCCCACCCCCTCCTCGCCGCCACAGCCCAACTTTCCAAC
GGCGCCCACCTCTTCACCGGACGCCTCTCCCTCGCCACCCACCCCTGGCTGGCCCATCAC
ACCATCCGCGGCACAGTCGTCCTGCCTGCCGCAGCTCTGGTGGACCTGGCCCTTCACGCG
TCCCACCACACCGGCCACGGCGGCATAGGCGACCTGACCCTCCACACGCCGCTCGCGCTC
GCGGAGGGCGCGGCCGTACGACTCCGTATCACGGTCGAGACAGCGAACGGCGACGCACCG
GCCACCCTCTCCATCGACTCACAACCGGAGTCGGACCCGGAGTCCGCCTGGACCACCCAT
GCATCCGCGACCCTCGGTCTCTCGCCCGCCGAGGGCGTGAACGAACAGCCCCTCTCGGTG
AATTGGCCTCCTCGGGAAGCCCGCAGCCGCGCTGTGGACGACCTGTACGAAGACCTCACG
GACGTCGGCTTGGCATACGCACCCGCGTTCCAGGGCGTCCGCGCCCTGTGGCAGCACGGT
GACACTCTCTACGTGGAGGTGGGCCTCCCGGAGGAGCCGACGCTCCCGGACACGGCCTGG
GACGGATTCGGCGTCCACCCGGCCCTCCTCGACGCCGCGCTCCATCCGCTGCTGCGCGCC
CACGGCGATGGCGGGCGCACCACCCTGCGTTGGTCGGGTATCTCGCTGCATGCCGAGCCC
AGCCCGGTGCTGCGAGTACGACTGACCCCGACCGCTGACGACACCTTCGCCGTCGAGATC
GCCGACGACACGGGCGCCTTGGTCCTCACAGCCGGTGCGGTACGACTGGGCCCTCTGCCC
GACTCCTTGCCGCGCACCGGTCGCCACGTCGCCCCGGGTGACGCCCTCTTCCGCGTCGGC
TGGAAGCCGGCTGCTCCGGAGAGCCGGGAGTTCGCTCCTGGGAGATGGGGGCTGCTCGGC
GAGGATTTGCTCGGTGTCCGGCCTGCCCTTGAGGCCGCCGGCCAGGTCGGCCGCATCGGC
TCGTATCCCGACATGAAGGCGCTGCTCTCGGTCCTCGACGACGTCGTACCGCAATCGGTC
ATCGCGCCCTGCGTCAGCCCGGAGGCCGACGACGTCGCCCAGGCCGTACACACCCTCACG
GAACGCGTCCTCAGCCTCCTTCAGTCCTGGCTCGCCGAGCCCCGGCTCACCGCCTCCCGG
CTGGTGGTCGTCACACGAGGAGCCGTGGCAGCCTCCGACACCGAGAAGCCGGACGATCTC
GGTGCGGCATCGGTCTGGGGTCTGCTGCGCAGCGCGCAGTCCGAGAATCCGGACCAGTTC
CAGCTCATCGACACAGACGGAACCGCCGCCTCCCTCACGGCTCTCGCCACCGTACTGACC
AGCGGCGAACCCCAGGTCGCCCTACGCGAGGGCAAGATGCTCACGCCGCACCTGACAAAG
GAACCGGTCGCGAAGGACGCCGTGGCACCGGCCCTGGACCCGGAGGGCACCGTCCTGATC
ACCGGCGGCACCGGCACCCTCGGCGCCCTCACCGCCCGCCACCTCATCACCCAGCACGGC
GCCCGCCGCCTCCTCCTGGCCAGCAGGCGAGGCCCCGAGGCCGACGGCGCCACCGAACTC
CACGATGGGCTCACCGAGTTGGGCGCCCACGTCACCATCACCGCCTGCGACACAGCCGAC
CCCGCCCAACTCGCCGACCTCCTCACCACCATCCCCACCGAACACCCCCTCACCGCAGTC
ATCCACACCGCAGGCATCACCCACGACGCCACACTCACCACCCTCACCCCCGAACAACTC
CACACCGTCCTCAAACCCAAAGTCGACGCCGCCTGGCACCTCCACCACCAAACCCAACAC
CTCCACCTCACCGCCTTCATCCTCTACTCCTCCGCCGCAGGCATCCTCGGCAACCCAGGC
CAAGCCAACTACGCCGCCGCCAACACCTTCCTCGACACCCTCGCCCACCACCGCCACACC
CAAGGACTCCCCGCCACCAGCCTCGCCTGGGGACTCTGGACCCAAACCAGCACCATCACC
GCCACCCTCGACAAGGACAGGGGCATCAGCTCCGCGGGCGTCACCGCACTTCCCACGGAC
CAGGCACTCGCCCTCTTCGACGCGGCACTGGCCAGCGGTCGCCCGCAGCTCACACCGATC
GGCCTCGATCTCCGTTCGCTGCGCGCCGCCGCCGAGGCCGGGGTCCTCCCCGCGCCGCTG
CGCACCCTCGTCGAACGGTCCGGACCCCGCAGGCGCGGCCGTACCGAACCCTCGCTGGCG
CGGCGCCTCGCCGGCCTGTCGGAAACCGAGCAGTCCGCTCTGCTCCTGGAGCTGGTGCGC
ACGCACACCGCTGCTGTGCTCGGGCACGCCTCAGCGGAAGCGATCGCCACTCAACGGGCT
TTCCATGAGCTGGGGTTCGACTCGCTGGCCGCGGTGAAGCTGCGCAATCAGCTGAACACC
GCCACGGGGCTCCGGCTCCCCATGACCGTGATCTTCGACCATCCCCATGTCGCCGCGCTC
GCGGACCGGCTGCACACGGAACTCCGTACCGAAGGCACCGCTCCCGGCGCTGCGCTCGCC
GAACTGGAGCGGTTCGAGGCGACCGTCCTGTCGCTGGCCCCGGACAGCGATCAGCGTTCC
GGGCTGGCGGCCCGCCTGCGCGCGCTGCTCTGGAAGCTCGACGCGGACCCGCACACCGCG
ACGCGGCAGGCAGCACCCGGGGACGACCTCGGGTCGGCCTCGGACGACGAGCTCTTCAGC
GTGCTGGACAACGAACTCGGCATCGCGCGCACCCACGACTGA
[8] KS36..413
[8] AT566..880
[8] malonyl-CoA758..762
[8] dh929..1093
[8] KR1416..1596
[8] ACP1695..1765
[8] KS106..1239
[8] AT1696..2640
[8] malonyl-CoA2272..2286
[8] dh2785..3279
[8] KR4246..4788
[8] ACP5083..5295

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[558-691] 2.90000000000003e-75 G3DSA:3.40.366.10 [758-871] 2.90000000000003e-75 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
[1723-1738] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1699-1764] 2.3e-11 PF00550
PF00550   PP-binding
[1695-1765] PS50075
PS50075   ACP_DOMAIN
[1695-1768] 7.90000000000001e-21 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1687-1782] 3.80000697093586e-21 SSF47336
SSF47336   ACP_like
IPR013968 Polyketide synthase, KR (Domain)
[1416-1595] 1.40000000000001e-63 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[36-287] 5.30000000000001e-99 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[295-413] 1.99999999999999e-47 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[566-880] 1.9e-66 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[563-868] 3.10001307370203e-68 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[693-757] 9.00000407957274e-18 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[35-297] 6.99999999999989e-92 G3DSA:3.40.47.10 [299-466] 1.99999999999999e-67 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[28-411] 2.50000909916183e-104 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1417-1617] 3.09999999999998e-37 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[200-216] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[567-862] 1.90000694315261e-125 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1696-1768] 3.80000697093586e-31 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
[929-1093] 3.39999972437719e-61 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[38-465] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1416-1596] 3.99998544139406e-59 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit
Chth_00290 Chth_00290   Chth_00310 Chth_00310
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