Chro_00180 : CDS information

Location

Organism	Streptomyces griseus
Strain	ATCC 13273 (=NBRC 3746)
Entry name	Chromomycin
Contig	AJ578458
Start / Stop / Direction	18,623 / 19,804 / + [in whole cluster] 18,623 / 19,804 / + [in contig]
Location	18623..19804 [in whole cluster] 18623..19804 [in contig]
Type	CDS
Length	1,182 bp (393 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	D-oliose glycosyltransferase
Product (GenBank)	glycosyltransferase
Gene
Gene (GenBank)	cmmGI
EC number
Keyword	disaccharide 2nd D-oliose
Note
Note (GenBank)
Reference	ACC Q70J80 PmId [15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004) [16391039] Deoxysugar transfer during chromomycin A3 biosynthesis in Streptomyces griseus subsp. griseus: new derivatives with antitumor activity. (Appl Environ Microbiol. , 2006) comment [PMID:15112992](2004) aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。 CmmGI : Glycosyltransferase -- [PMID:16391039](2006) chromomycin gene clusterにある4つのglycosyltransferase genes (cmmGI, cmmGII, cmmGIII, and cmmGIV)の不活化mutant作製、産物解析。 cmmGII mutantはposition 8でのdisaccharide鎖を欠いた4環化合物産生。 cmmGI mutantはposition 8でのdisaccharide鎖の2つめの糖を欠いた3環化合物を産生。よって、生合成中間体prechromomycin A3の8 positionへCmmGII が1つめ、CmmGI が2つめのD-oliosyl基を取り込むことによりdisaccharideの形成を担う。

Sequence

>D-oliose glycosyltransferase [glycosyltransferase]
MRVLLTTAAWPSHYMFMVPLAWAFRAAGHEVRVAAQPHVVETVLKSGLTAVPVGPDVDFA
QLHRKFVAETDPAQYRSRDAIITIFHRVAEMMAPDLVRFAESWKPDLVIRDPVAFAASVA
AEVSGAPLVRHAWGPDVYATDPGKWLAHHVLERLGPTYELYGVRPPDTFDRLAVDPTPPS
LHLDASGPSLPLRYVPYNGPGVVPPWVLGTPDRPRVCVTWGTFTSTATAELDMFLVPTVL
DALAGLDVEPVVAVTGAERELLGDVGGRARIVQDLPIHTVLPSCSAVVHHGGATTVLSAA
RFGVPQLTMPHLFEQRLNSDLLEAAGAGVQLTAAHADAESIGAAVTELLRGDAPYAVASR
GLRDEIEAMPSPLETVALIEETLPRGARADTGD

>D-oliose glycosyltransferase [glycosyltransferase]
ATGCGTGTTCTGCTGACCACCGCGGCCTGGCCGTCGCACTACATGTTCATGGTCCCGCTC
GCCTGGGCCTTTCGCGCCGCCGGGCACGAGGTGCGCGTCGCGGCCCAGCCGCACGTGGTG
GAGACCGTGCTGAAGTCGGGCCTGACCGCTGTACCCGTGGGCCCCGACGTCGACTTCGCC
CAGCTGCACCGGAAGTTCGTCGCCGAGACCGACCCCGCCCAGTACCGGAGCCGGGACGCC
ATCATCACGATCTTCCACCGGGTCGCGGAGATGATGGCCCCCGATCTCGTTCGGTTCGCC
GAGTCCTGGAAGCCCGACCTCGTCATCCGGGACCCGGTGGCCTTCGCGGCGTCCGTCGCC
GCCGAGGTGTCCGGCGCCCCGCTGGTCCGGCACGCGTGGGGCCCCGACGTGTACGCCACC
GACCCGGGGAAGTGGCTCGCGCACCACGTCCTGGAACGGCTCGGCCCCACCTACGAGTTG
TACGGGGTACGGCCGCCGGACACCTTCGACCGGCTCGCCGTCGACCCGACCCCGCCGAGC
CTGCACCTCGACGCCTCCGGCCCGTCGCTGCCCCTGCGCTACGTGCCGTACAACGGGCCG
GGCGTGGTGCCGCCCTGGGTCCTCGGAACACCGGACCGCCCCAGGGTCTGCGTCACGTGG
GGCACCTTCACGTCCACCGCGACGGCCGAGCTGGACATGTTCCTGGTCCCGACGGTGCTC
GACGCCCTCGCCGGCCTGGACGTGGAGCCGGTAGTCGCGGTGACCGGGGCGGAGCGGGAG
CTGCTGGGCGACGTGGGAGGCCGGGCCAGGATCGTGCAGGACCTCCCCATCCACACGGTG
CTGCCCAGCTGCTCCGCCGTGGTCCACCACGGCGGAGCGACGACCGTCCTGTCCGCGGCC
CGCTTCGGAGTGCCCCAGCTGACCATGCCGCACCTGTTCGAGCAACGCCTCAACTCGGAC
CTGCTGGAGGCGGCCGGTGCCGGTGTCCAGCTCACCGCGGCGCACGCCGACGCCGAGTCC
ATCGGTGCCGCGGTGACGGAGCTGCTTCGCGGGGACGCGCCGTACGCGGTGGCCTCGCGC
GGGCTGCGGGACGAGATCGAGGCCATGCCGTCCCCGCTCGAGACCGTCGCCCTCATCGAG
GAGACGCTCCCGCGCGGTGCGCGCGCGGACACCGGAGACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR004276 Glycosyl transferase, family 28 (Domain) PF03033 Glyco_transf_28 IPR010610 Domain of unknown function DUF1205 (Domain) PF06722 DUF1205
SignalP	Eukaryota Bacteria, Gram-negative Bacteria, Gram-positive
TMHMM	No significant hit

Chro_00170 Chro_00190