Chro_00290 : CDS information

Location

Organism	Streptomyces griseus
Strain	ATCC 13273 (=NBRC 3746)
Entry name	Chromomycin
Contig	AJ578458
Start / Stop / Direction	33,508 / 32,240 / - [in whole cluster] 33,508 / 32,240 / - [in contig]
Location	complement(32240..33508) [in whole cluster] complement(32240..33508) [in contig]
Type	CDS
Length	1,269 bp (422 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	hexose O-acetyltransferase
Product (GenBank)	acetyltransferase
Gene
Gene (GenBank)	cmmA
EC number	2.3.1.-
Keyword	trisaccharide 3rd L-chromose disaccharide 1st D-oliose
Note
Note (GenBank)
Reference	ACC Q70J69 PmId [15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004) [15255901] Tailoring modification of deoxysugars during biosynthesis of the antitumour drug chromomycin A by Streptomyces griseus ssp. griseus. (Mol Microbiol. , 2004) [21342468] The chromomycin CmmA acetyltransferase: a membrane-bound enzyme as a tool for increasing structural diversity of the antitumour mithramycin. (Microb Biotechnol. , 2011) comment [PMID:15112992](2004) aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。 CmmA: O-acyltransferase 配列解析から機能推定。CmmAは、macrolide aglyconeでも、macrolide aglycone付着済みのsugarsでも働くacyltransferasesに似ている。よってdisaccharide鎖の1つめの糖D-olioseのC-4 OH基 and/or trisaccharide鎖の3つめの糖L-chromoseのC-4' OH基にacetyl基を移すと考えられている。 -- [PMID:15255901](2004) cmmA不活化mutantは、chromomycin A3に含まれる2つのacetyl基を両方持たないdideacetylated chromomycin A3派生体を蓄積。この化合物は、cmmA発現S.albusにおいて4E-monoacetylated中間体を介してchromomycin A3へと変換される。このacetylationはchromomycin生合成で最終stepであり、産生生物の自己抵抗性のために重要。 4E-のEはsugar E。trisaccharideの3rd sugar L-chromoseのこと。つまり先にsugar E(L-chromose)が、次にsugar A(D-oliose)が4-OH基でacetyl基を取り込む。 -- [PMID:21342468](2011) cmmAを用いたS.griseus操作株は、抗癌活性の高い新規acetylated mithramycinsを産生。 CmmA acetyltransferaseは細胞膜に位置し、いくつかのacyl-CoA基質を受け入れる。

Sequence

>hexose O-acetyltransferase [acetyltransferase]
MISPVVENTAKNNAAVTHLVSLTGLRGILALMVFGTHFFLAFVMAGGSMDSPFVQQVFHV
LFSRAGGTAVSCFFLLSGFVLAYIARPGDSTRSFYRRRIAKIYPVHLISTAVAFILVSVR
YEVPGMEVTLSHVFLVQSWVPSQWYYLTMTGIDWSLSCEAFFYLCFPVLLPVLSRARNGM
LYAVSACSVFLVFFLPYVVGHTFAIRSPDPVEMVPTAGYGGPIGYWFAYVFPPMRMAEFV
IGISLAVLVRRGAWRGPGVAACLVLCAIGWGVNLHLDSYLPLAAGMLIPFALLIPALSTA
DVNSAWSPLRWKPLVWFGEISLSFYVSHLLVQEELFSRLWNLGMRAGLLPAPLPVLSWWV
AVLSFLAQFALAVLAAWLLHRLVEQPLVRRLRPKDVPPVPVTSPQSSAPPAHQAGAAHMV
ER

>hexose O-acetyltransferase [acetyltransferase]
ATGATTTCCCCGGTTGTCGAGAATACAGCCAAGAATAATGCGGCGGTCACCCATCTCGTG
TCCCTCACAGGACTGCGCGGAATTCTCGCGTTGATGGTGTTCGGCACGCATTTCTTTCTC
GCGTTCGTCATGGCGGGCGGGTCGATGGACTCGCCGTTCGTACAGCAGGTGTTCCACGTG
CTGTTCTCCCGGGCCGGCGGAACGGCGGTCTCCTGCTTCTTCCTGCTCAGCGGGTTCGTC
CTGGCCTACATCGCACGCCCGGGTGACAGCACCCGCTCCTTCTACCGGCGCCGGATCGCG
AAGATCTATCCGGTCCACCTGATCAGCACCGCCGTCGCGTTCATCCTGGTCTCGGTGCGG
TACGAGGTGCCCGGCATGGAGGTCACGCTGTCCCATGTGTTCCTGGTGCAGAGCTGGGTC
CCGAGCCAGTGGTACTACCTCACGATGACGGGCATCGACTGGTCGCTGAGCTGTGAGGCG
TTCTTCTACCTGTGCTTCCCCGTACTGCTTCCGGTGCTGAGCAGGGCCAGGAACGGGATG
CTCTACGCGGTGTCCGCGTGCTCGGTGTTCCTGGTCTTCTTCCTGCCGTACGTCGTCGGA
CACACCTTCGCGATCCGCTCGCCCGATCCGGTGGAGATGGTGCCGACCGCCGGATACGGC
GGGCCGATCGGCTACTGGTTCGCGTATGTCTTCCCGCCCATGCGCATGGCCGAGTTCGTC
ATCGGGATCTCCCTGGCCGTCCTGGTGCGCAGGGGCGCGTGGCGCGGACCGGGGGTCGCG
GCCTGCCTGGTGCTGTGCGCGATCGGCTGGGGGGTCAATCTCCACCTCGACAGCTATCTG
CCGCTCGCGGCGGGCATGCTCATCCCCTTCGCCCTGCTCATCCCCGCCCTCAGCACCGCC
GACGTCAACTCCGCCTGGTCGCCGCTCCGCTGGAAACCGCTGGTGTGGTTCGGCGAGATA
TCCCTGTCCTTCTACGTCAGCCATCTGCTGGTCCAGGAGGAGCTGTTCAGCCGGCTGTGG
AACCTCGGCATGCGGGCGGGTCTGCTGCCCGCGCCGCTGCCCGTGCTGTCCTGGTGGGTG
GCGGTGCTGTCGTTCCTCGCCCAGTTCGCGCTGGCTGTGCTGGCCGCCTGGCTGCTCCAC
CGGCTGGTGGAACAGCCGCTGGTGCGCAGGCTGCGGCCGAAGGACGTCCCCCCGGTTCCG
GTGACCTCGCCCCAGTCGTCGGCCCCTCCCGCACATCAGGCCGGTGCGGCACACATGGTG
GAGAGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002656 Acyltransferase 3 (Domain) PF01757 Acyl_transf_3
SignalP	Eukaryota
TMHMM	Transmembrane 10

Chro_00280 Chro_00300