Cosmo_00010 : CDS information

Location

Organism	Streptomyces olindensis
Strain	DAUFPE 5622
Entry name	Cosmomycin
Contig	DQ280500
Start / Stop / Direction	365 / 3 / - [in whole cluster] 365 / 3 / - [in contig]
Location	complement(3..365) [in whole cluster] complement(3..365) [in contig]
Type	CDS
Length	363 bp (120 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose C-4 ketoreductase(fragment)
Product (GenBank)	CosM
Gene
Gene (GenBank)	cosM
EC number
Keyword
Note
Note (GenBank)	putative NDP-hexose 4-ketoreductase
Reference	ACC Q2PZS5 PmId [16810496] Insights in the glycosylation steps during biosynthesis of the antitumor anthracycline cosmomycin: characterization of two glycosyltransferase genes. (Appl Microbiol Biotechnol. , 2006) comment cosmomycinsを含むanthracycline複合体retamycinの産生株Streptomyces olindensisの染色体14kb領域から、cosmomycin生合成に関連した13genesを同定。 cosM: NDP-hexose 4-ketoreductase cosMについては配列解析のみ。AclMと高い類似性あり。 cosMはL-rhodosamine or 2-deoxy-L-fucoseのどちらかの生合成の際に最終ketoreduction stepに関連すると提唱。
Related Reference	ACC O33708 NITE Adria_00200 PmId [9209071] Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine. (J Bacteriol. , 1997) [10631513] A two-plasmid system for the glycosylation of polyketide antibiotics: bioconversion of epsilon-rhodomycinone to rhodomycin D. (Chem Biol. , 1999) [9447597] Production of the antitumor drug epirubicin (4'-epidoxorubicin) and its precursor by a genetically engineered strain of Streptomyces peucetius. (Nat Biotechnol. , 1998) comment Blast 8th, id49%, 9e-17 Streptomyces peucetius_dnmV Thymidine diphospho-4-keto-2,3,6-trideoxyhexulose reductase [DoBISCUIT]NDP-hexose C-4 ketoreductase -- [PMID: 9209071](1997) dnmV: 4-keto基の還元 dnmV不活化mutant産物解析と相補による回復から、daunosamine生合成に関与することは確認されている。dTDP-4-ketodeoxyhexulose ketoreductase機能の割り当ては配列解析に基づく。 -- [PMID: 10631513](1999) 異種性ホストにおけるL-daunosamineの生合成と付着のための最少の情報は、dnmLMJVUTS genesによってコードされる。 DnmVとavrE and eryBIV genesの産物は、DNR and DXRに由来する4'-epimeric anthracyclinesの合成を通して、dTDP-4-ketohexulose reductasesであることが証明されている(ref26)。 ↓そのref26 [PMID: 9447597](1998) abstract S.peucetius dnmV mutantに、Streptomyces avermitilis avrE or Saccharopolyspora eryBIV genesを異種性に導入すると、epirubicin (4'-epidoxorubicin) and 4'-epidaunorubicinを形成。 Saccharopolyspora eryBIV = dTDP-4-keto-6-deoxy-L-hexose 4-reductase

Sequence

>putative NDP-hexose C-4 ketoreductase(fragment) [CosM]
MSTTTQGGAAVGTVAVLGATGCVGRSVCDELSRTGHRVLAVARHPAPHTRPHAFVPLDVA
GTAPERLAALLDRYGVTAVVNATGGWGTTVEEMRRAHIDLLERLVDGCAATSRRLRVVQL
G

>putative NDP-hexose C-4 ketoreductase(fragment) [CosM]
ATGTCCACCACCACGCAGGGCGGGGCGGCCGTCGGCACCGTGGCCGTGCTCGGCGCCACG
GGGTGCGTCGGCCGCAGCGTCTGTGACGAACTGTCGCGCACCGGGCACCGGGTGCTGGCG
GTGGCCCGGCACCCGGCGCCCCACACCCGCCCGCACGCCTTCGTCCCCCTCGACGTCGCC
GGGACCGCGCCGGAGCGTCTCGCCGCGCTCCTCGACCGGTACGGCGTCACCGCGGTGGTC
AATGCCACCGGCGGCTGGGGCACCACCGTCGAGGAGATGCGCCGGGCCCACATCGACCTC
CTGGAGCGCCTGGTCGACGGCTGTGCCGCGACCTCCCGGCGGCTGCGGGTCGTCCAGCTG
GGG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 no description
SignalP	Eukaryota
TMHMM	No significant hit

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