Cosmo_00020 Cosmo_00020   Cosmo_00040 Cosmo_00040

Cosmo_00030 : CDS information

close this sectionLocation

Organism
StrainDAUFPE 5622
Entry nameCosmomycin
Contig
Start / Stop / Direction2,366 / 1,074 / - [in whole cluster]
2,366 / 1,074 / - [in contig]
Locationcomplement(1074..2366) [in whole cluster]
complement(1074..2366) [in contig]
TypeCDS
Length1,293 bp (430 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductL-2-deoxyfucose glycosyltransferase
Product (GenBank)CosK
Gene
Gene (GenBank)cosk
EC number
Keyword
Note
Note (GenBank)
  • glycosyltransferase
Reference
ACC
PmId
[16810496] Insights in the glycosylation steps during biosynthesis of the antitumor anthracycline cosmomycin: characterization of two glycosyltransferase genes. (Appl Microbiol Biotechnol. , 2006)
comment
cosmomycinsを含むanthracycline複合体retamycinの産生株Streptomyces olindensisの染色体14kb領域から、cosmomycin生合成に関連した13genesを同定。

cosK: Glycosyltransferase

aclacinomycin生合成で2-deoxy-L-fucoseの付加を担うAknKに似ていることから、glycosyltransferase CosKはcosmomycin D生合成で2つめのdeoxysugarである2-deoxy-L-fucoseの取り込みを担うと考えられた。

これを検証するために作製されたcosK不活化mutantは、cosmomycinsではない4つのanthracycline化合物を産生。
これらはすべてaglyconeに付着したL-rhodosamineを含むので、CosKは糖鎖の2つめのdeoxysugarの移動に関与すると考えられる。
Related Reference
ACC
Q9L555
NITE
Acla_00030
PmId
[15078101] AknK is an L-2-deoxyfucosyltransferase in the biosynthesis of the anthracycline aclacinomycin A. (Biochemistry. , 2004)
[17685523] Characterization of rhodosaminyl transfer by the AknS/AknT glycosylation complex and its use in reconstituting the biosynthetic pathway of aclacinomycin A. (J Am Chem Soc. , 2007)
comment
Blast 3rd, id65%, 1e-157
Streptomyces galilaeus_aknK
AknK
[DoBISCUIT]L-2-deoxyfucose glycosyltransferase

--
[PMID: 15078101](2004) abstract
AknKを異種性発現して精製、実験に使用。
dTDP-L-2-deoxyfucoseとrhodosaminyl aklavinoneから、AknKはL-2-deoxyfucosyl-L-rhodosaminyl aklavinoneを生成。
dTDP-L-daunosamineなどでも作用し、anthracycline骨格で2糖目を組み立てる。
活性は下がるが、rhodosaminyl aklavinoneにdTDP-L-2-deoxyfucoseをtandemに追加して、L-deoxyfucosyl-L-deoxyfucosyl-L-rhodosaminyl aklavinoneを産生することもできる。

--
[PMID: 17685523](2007)
AknSはゆっくりな基質としてdTDP-2-deoxyfucoseを受け入れるけど、AknKはdTDP-L-rhodosamineを利用できない。

close this sectionSequence

selected fasta
>L-2-deoxyfucose glycosyltransferase [CosK]
MRVLFTTFAAKSHMHAQVTLAWALKAAGHDVRIASHPDLADEITRTGLTAVGVGEELHLD
EQMQETQENIGQDVDQLESQAQVGLDMSETRPERLTWDYTLGVFTAMTSVVFQNCCSDQM
VDELVEHCRSWKPDLVLWDTMTFAGAIAAMATGTAHGRLLFGMDLAGKMRETFLEQLAAR
PPELRDDPLREWLTWSLERHGASFEEAAVTGDFTVCPLPASMRFPLDLHYVPMRYVPYNG
QSTVPQWLQEPPKRGRRVCITLGVAHREVLGADRASIGDLLEAVAGLDDVEVVATLNENQ
LKDFDTLPDNVRAVDFVPLDALLPTCDAVIHHGGSGSFGTAMIHAVPQLIVVDGVWDTIN
KARYLERRQAGLYVPGKLSAKGLRELLVRLLEEPVFKRNAEALRREALAAPAPAQVVPTL
ERLAAEHRRR
selected fasta
>L-2-deoxyfucose glycosyltransferase [CosK]
ATGCGGGTACTCTTCACCACCTTCGCCGCCAAGTCGCACATGCACGCCCAGGTCACACTC
GCCTGGGCCCTCAAGGCGGCGGGCCACGACGTCAGGATCGCCAGTCACCCCGATCTCGCC
GACGAGATCACCCGTACCGGGCTGACCGCCGTCGGGGTCGGGGAGGAACTGCATCTCGAC
GAGCAGATGCAGGAGACCCAGGAGAACATCGGCCAGGACGTCGACCAGCTGGAGAGCCAG
GCACAGGTCGGTCTCGACATGAGCGAGACCCGCCCGGAGCGGCTCACCTGGGACTACACA
CTCGGTGTGTTCACGGCCATGACCTCGGTCGTCTTCCAGAACTGCTGCTCCGACCAGATG
GTCGACGAGCTGGTGGAGCACTGCCGCTCCTGGAAGCCCGACCTGGTCCTGTGGGACACC
ATGACCTTCGCGGGCGCCATCGCCGCCATGGCCACGGGGACGGCGCACGGCCGGCTGCTC
TTCGGGATGGACCTGGCGGGCAAGATGCGCGAGACCTTCCTGGAGCAGCTGGCGGCGCGT
CCGCCGGAGCTGCGGGACGACCCGCTGCGCGAGTGGCTCACCTGGAGCCTGGAGCGGCAC
GGCGCCTCCTTCGAGGAGGCCGCGGTGACGGGCGACTTCACCGTCTGCCCGCTGCCGGCG
TCCATGCGGTTCCCGCTGGACCTGCACTACGTGCCGATGCGGTACGTGCCGTACAACGGC
CAGTCCACCGTGCCGCAGTGGCTCCAGGAACCGCCGAAGCGGGGCCGCCGGGTGTGCATC
ACGCTGGGTGTGGCGCACCGCGAGGTGCTGGGCGCCGACCGGGCGTCCATCGGCGACCTG
CTGGAGGCCGTCGCGGGGCTCGACGACGTCGAGGTCGTCGCCACGCTCAACGAGAACCAG
CTGAAGGACTTCGACACCCTGCCGGACAACGTGCGGGCCGTCGACTTCGTCCCGCTCGAC
GCGCTGCTGCCCACCTGCGACGCGGTGATCCACCACGGCGGGTCGGGCAGCTTCGGCACG
GCGATGATCCACGCCGTGCCCCAGCTCATCGTCGTCGACGGCGTCTGGGACACCATCAAC
AAGGCCCGCTACCTGGAACGGCGCCAGGCCGGGCTCTACGTGCCCGGCAAGCTCAGCGCG
AAGGGACTGCGCGAGCTGCTCGTCCGCCTGCTCGAGGAACCGGTCTTCAAGCGCAACGCC
GAGGCCCTGCGGCGCGAGGCGCTCGCCGCTCCCGCCCCCGCGCAGGTCGTCCCCACGCTG
GAGCGCCTGGCCGCCGAGCACCGCCGCCGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR004276 Glycosyl transferase, family 28 (Domain)
[3-117] 1.9e-11 PF03033
PF03033   Glyco_transf_28
IPR010610 Domain of unknown function DUF1205 (Domain)
[217-312] 2.1e-31 PF06722
PF06722   DUF1205
SignalP
[1-28] 0.85 Signal
Eukaryota   
TMHMM No significant hit
Cosmo_00020 Cosmo_00020   Cosmo_00040 Cosmo_00040
Page top