Ello_00040 : CDS information

Location

Organism	Streptomyces olivaceus
Strain	Tü2353
Entry name	Elloramycin
Contig	AM900040
Start / Stop / Direction	1,881 / 2,807 / + [in whole cluster] 1,881 / 2,807 / + [in contig]
Location	1881..2807 [in whole cluster] 1881..2807 [in contig]
Type	CDS
Length	927 bp (308 aa)

Annotation

Category	3.2 modification methylation
Product	putative methyltransferase
Product (GenBank)	C12a O-methyltransferase
Gene
Gene (GenBank)	elmD
EC number	2.1.1.-
Keyword
Note	The N-terminal position was modified from original INSDC entry.
Note (GenBank)
Reference	ACC A8KQU1 PmId [18310024] Biosynthesis of elloramycin in Streptomyces olivaceus requires glycosylation by enzymes encoded outside the aglycon cluster. (Microbiology. , 2008) comment elloramycin clusterの同定論文。 elmDについては配列解析のみ。 elmD: C12a O-methyltransferase elloramycin生合成での最終ステップであろうC12aでのmethoxy基形成を担うと提唱されている。
Family/Domain	FD IPR007213, IPR011610 comment [IPR007213] Leucine carboxyl methyltransferase (Family) [IPR011610] S-adenosyl-L-methionine-dependent methyltransferase, putative (Family) function; methyltransferase activity

Sequence

>putative methyltransferase [C12a O-methyltransferase]
MTAKYEEWDISVSVGLTALAACSARAVESDRPDRLIEDPYARDLVLAARKPRPLPTSLAE
ADKAAGSMMTSRFTGVRSRVFDDFFAAAGRSGIRQAVVLAAGLDARAFRLPWPSGTVVYE
IDQPGVLRYKEEVMAHLGVTPTCVRRPVGRDLRTDWASGLGAAGFDPTRPTAWIGEGLLP
YLPAEAEAEMFASIRALSAPGSRLCVEFMPAEVVARFAAHPTMRGTSDSLGVDIGEMWNS
EPRQAAADRLRETGWNVTVDTVLDRARELGAPLDEEFAGIVDMLGVPRESPLAATFGACA
FVAAELPA

>putative methyltransferase [C12a O-methyltransferase]
ATGACTGCCAAGTACGAAGAGTGGGACATCAGTGTCAGTGTGGGTTTGACGGCTCTCGCC
GCGTGTTCGGCGCGTGCGGTGGAGAGTGACCGGCCCGACCGGCTCATCGAAGACCCTTAC
GCGAGGGATCTGGTGCTCGCCGCCCGAAAGCCCAGGCCGCTTCCGACGTCGTTAGCGGAG
GCCGACAAAGCCGCGGGTTCGATGATGACGTCGCGTTTCACCGGGGTGCGTTCCCGGGTC
TTCGACGACTTCTTCGCCGCGGCGGGACGGTCCGGCATACGCCAGGCCGTCGTGCTCGCG
GCCGGCCTCGACGCCCGTGCGTTCCGGCTGCCCTGGCCGTCCGGCACGGTCGTGTACGAG
ATCGACCAGCCCGGCGTCCTGCGGTACAAGGAGGAGGTCATGGCGCATCTGGGCGTCACG
CCGACCTGTGTCCGGCGCCCGGTGGGCCGGGACCTGCGCACCGACTGGGCGAGCGGCCTC
GGAGCCGCCGGCTTCGACCCGACCCGGCCCACGGCATGGATCGGCGAGGGGCTCCTCCCG
TATCTGCCGGCCGAGGCCGAGGCCGAGATGTTCGCGTCGATCAGGGCCCTCTCCGCACCC
GGCAGCCGGTTGTGCGTGGAGTTCATGCCGGCCGAGGTCGTCGCACGCTTCGCCGCCCAT
CCGACCATGCGGGGCACGAGCGACTCGCTCGGTGTCGACATCGGCGAGATGTGGAACTCC
GAGCCCCGCCAGGCCGCCGCCGACCGGCTGCGGGAAACCGGCTGGAACGTGACGGTCGAC
ACGGTCCTCGACCGTGCCCGTGAGCTCGGGGCGCCGCTCGACGAGGAGTTCGCCGGAATC
GTCGACATGCTGGGCGTGCCCAGGGAGAGCCCGCTCGCCGCGACCTTCGGCGCCTGTGCG
TTCGTGGCCGCCGAACTGCCTGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR007213 Leucine carboxyl methyltransferase (Family) PF04072 LCM IPR011610 S-adenosyl-L-methionine-dependent methyltransferase, putative (Family) TIGR00027 mthyl_TIGR00027: putative methyltransferase, TIGR0
SignalP	No significant hit
TMHMM	No significant hit

Ello_00030 Ello_00050