Ello_00050 : CDS information

Location

Organism	Streptomyces olivaceus
Strain	Tü2353
Entry name	Elloramycin
Contig	AM900040
Start / Stop / Direction	3,099 / 4,658 / + [in whole cluster] 3,099 / 4,658 / + [in contig]
Location	3099..4658 [in whole cluster] 3099..4658 [in contig]
Type	CDS
Length	1,560 bp (519 aa)

Annotation

Category	4.3 transport
Product	putative drug resistance transporter
Product (GenBank)	elloramycin permease
Gene
Gene (GenBank)	elmE
EC number
Keyword
Note
Note (GenBank)
Reference	ACC A8KQU7 PmId [18310024] Biosynthesis of elloramycin in Streptomyces olivaceus requires glycosylation by enzymes encoded outside the aglycon cluster. (Microbiology. , 2008) comment elloramycin clusterの同定論文。 elmEについては配列解析のみ。 elmE: Elloramycin permease elloramycinの分泌を担うmembrane transporterをコードすると提唱している。
Related Reference	ACC Q0R4N5 NITE Chth_00190 PmId [7916693] The pur8 gene from the pur cluster of Streptomyces alboniger encodes a highly hydrophobic polypeptide which confers resistance to puromycin. (Eur J Biochem. , 1993) comment Blast list外、id32.84%, 1e-41 Streptomyces alboniger_pur8 Puromycin resistance protein pur8 puromycin合成clusterからpuromycin-resistance gene_pur8を分離、Streptomyces lividansで発現させ、抗生物質抵抗性を検討。puromycinに高く特異的。 TMSs予測は14。上流ORFは逆向きで、resistanceに関与しない。

Sequence

>putative drug resistance transporter [elloramycin permease]
MASIDSGLGAAQAGDTASHPTKLDGPLLKVAAAVIAGSIMAILDTTVVTIALQSLIVEFH
TSFDTVQWVTTAYMLALAAVIPVTGWAADRFGTKRLYALALTLFLVGSVLAGFAWNIGSM
IAFRVLQGLGGGILTPAGMTVLAKAAGPARVGRVMSLLGVPMLLGPLAGPIVGGWLVDSG
GWRWIFYLNVPIGLLALALTLRYLPKDTETTGGALDTVGTLLLSPGVALVLYGVSGIPRS
GGITPGVWLPVALGLLMIAGFVVRAQRIEHPLLDLSLFRNRRFSLAVVTMALFAVAFFGV
SILLPTEFLMVRQLSALETGMQLLPLGIGAVVTMPLSGRLADRVGPGKVVVPGLLLIAAG
ITVFTQAGPDSGYATVLAPGLFVIGLGVGATMMPIISAALQVLDGPAVARGSTMANIIQQ
GFGAIGSAVMSIVLAAGLAGRFDVPVSQGQLVATTALADPARHATALGMVSDTFTTTFVW
SLALVILCLVPACFLWRKPAEAVSAPVPRLKAGTVDGLS

>putative drug resistance transporter [elloramycin permease]
ATGGCGTCAATCGACTCCGGCCTGGGCGCTGCCCAGGCCGGTGACACCGCTTCCCACCCC
ACCAAGCTCGACGGCCCGCTGCTCAAGGTCGCGGCCGCCGTGATCGCCGGATCGATCATG
GCGATCCTGGACACGACCGTCGTGACGATCGCGCTGCAGAGCCTGATCGTCGAGTTCCAC
ACCTCGTTCGACACCGTCCAGTGGGTCACCACCGCCTACATGCTGGCTCTGGCCGCGGTG
ATCCCCGTGACCGGCTGGGCCGCCGACCGCTTCGGTACGAAGCGGCTGTACGCGCTCGCG
CTCACGCTGTTCCTGGTCGGCTCGGTCCTGGCCGGGTTCGCCTGGAACATCGGCTCGATG
ATCGCGTTCCGCGTCCTCCAGGGCCTGGGCGGCGGCATCCTCACCCCGGCCGGCATGACG
GTCCTGGCCAAGGCGGCGGGCCCCGCCAGGGTCGGGCGCGTCATGTCGCTGCTGGGCGTC
CCGATGCTGCTCGGCCCGCTGGCGGGGCCGATCGTCGGCGGCTGGCTCGTCGACTCCGGC
GGCTGGCGCTGGATCTTCTACCTCAACGTGCCCATCGGCCTGCTCGCCCTCGCCCTGACC
CTGCGCTACCTGCCCAAGGACACGGAGACGACCGGCGGCGCGCTCGACACGGTCGGCACC
CTCCTGCTGTCGCCCGGGGTGGCCCTCGTGCTCTACGGCGTGTCCGGCATCCCGCGGTCC
GGTGGCATCACCCCCGGGGTGTGGCTTCCGGTCGCCCTCGGCCTGCTCATGATCGCGGGC
TTCGTCGTCCGGGCCCAACGGATCGAGCACCCGCTGCTCGACCTCTCCCTCTTCCGCAAC
CGACGTTTCTCCCTCGCCGTCGTCACAATGGCGCTCTTCGCGGTCGCCTTCTTCGGGGTC
AGCATCCTGCTGCCCACCGAGTTCCTGATGGTGCGTCAACTCAGTGCGCTGGAAACCGGC
ATGCAGCTGCTGCCGCTCGGCATCGGCGCGGTCGTGACCATGCCGCTGTCCGGCCGGCTC
GCCGACCGCGTCGGGCCCGGCAAGGTCGTCGTACCCGGTCTGCTGCTGATCGCGGCCGGC
ATCACCGTCTTCACCCAGGCCGGTCCCGACAGCGGGTACGCGACCGTGCTCGCCCCCGGG
CTCTTCGTCATCGGCCTCGGCGTCGGCGCCACGATGATGCCGATCATCTCCGCGGCGCTC
CAGGTCCTGGACGGGCCCGCCGTCGCGCGGGGGTCGACCATGGCCAACATCATCCAGCAG
GGGTTCGGCGCCATCGGGTCCGCGGTGATGTCGATCGTGCTCGCGGCCGGGCTCGCCGGC
CGGTTCGACGTCCCCGTCAGCCAGGGGCAGTTGGTCGCCACCACGGCGCTGGCCGATCCC
GCGCGGCACGCGACCGCGCTCGGCATGGTCTCCGACACCTTCACCACGACCTTCGTCTGG
TCACTGGCCCTCGTGATCCTGTGCCTGGTGCCCGCCTGCTTCCTGTGGCGGAAGCCGGCC
GAGGCCGTCTCCGCGCCGGTGCCCCGCCTGAAGGCGGGTACTGTTGACGGCCTTTCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR004638 Drug resistance transporter EmrB/QacA subfamily (Family) TIGR00711 Efflux_EmrB IPR011701 Major facilitator superfamily (Family) PF07690 MFS_1 IPR016196 Major facilitator superfamily domain, general substrate transporter (Domain) SSF103473 MFS_gen_substrate_transporter IPR020846 Major facilitator superfamily domain (Domain) PS50850 MFS
SignalP	No significant hit
TMHMM	Transmembrane 14

Ello_00040 Ello_00060