Ello_00060 Ello_00060   Ello_00080 Ello_00080

Ello_00070 : CDS information

close this sectionLocation

Organism
StrainTü2353
Entry nameElloramycin
Contig
Start / Stop / Direction6,162 / 7,781 / + [in whole cluster]
6,162 / 7,781 / + [in contig]
Location6162..7781 [in whole cluster]
6162..7781 [in contig]
TypeCDS
Length1,620 bp (539 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.1 modification hydroxylation
Producthydroxylase
Product (GenBank)TcmB2 oxygenase
Gene
Gene (GenBank)elmG
EC number
Keyword
  • C-4
  • C-4a
  • C-12a
Note
Note (GenBank)
Reference
ACC
PmId
[11325225] Cloning, sequencing, and heterologous expression of the elmGHIJ genes involved in the biosynthesis of the polyketide antibiotic elloramycin from Streptomyces olivaceus Tu2353. (J Nat Prod. , 2001)
[18310024] Biosynthesis of elloramycin in Streptomyces olivaceus requires glycosylation by enzymes encoded outside the aglycon cluster. (Microbiology. , 2008)
comment
[PMID:11325225](2001)
Elloramycin A産生に関連するcosmid clone 16F4からの遺伝子同定、配列解析とその遺伝子の異種性発現。

ElmG: tetracenomycin B2 oxygenase

elloramycinのaglycon 8-demethyltetracenomycin C (8-DMTC)は、tetracenomycin Cと8-OH基が違うだけ。よってElmGは、tetracenomycin(Tcm) B2 → 8-DMTCへの変換を担うと提唱されている。
この反応は、C-4, C-4a, C-12aでのtriple hydroxylation.

ElmGの基質として提唱されているTcmB2は入手不可能なので、ElmG酵素活性測定の基質にはTcmA2を使用。S.lividansで過剰発現、精製されたElmGは、TcmA2を同じ場所でtriple hydroxylationしたTcmCへと変換することをHPLC測定にて確認している。NADP or NADPHをelectron donorとして利用し、O2分子を必要とする。

8-DMTCがelloramycin aglyconeであることが確認されていることからTcmB2が直接triple hydroxylationされると判断し、基質名をTcmB2としている。

---
[PMID:18310024](2008)
elloramycin clusterの同定論文。
elmGに関しては上記論文をRefとして引用。

elmG: TcmB2 oxygenase
Related Reference
ACC
P39888
PmId
[8509339] ( , )
comment
tcmP and tcmGの配列解析、異種性発現。
tcmGはtetracenomycin (TCM) A2のpositions C-4, C-4a, C-12aでのhydroxylationを担い、TCM Cを形成する。

close this sectionSequence

selected fasta
>hydroxylase [TcmB2 oxygenase]
MDRIEIPVLVVGGGLTGLAAAVFLRQQGVDCLLVERHRSTTFLTRASGINARTMELLRNA
GLEETVIDRSLHLIEGKRWRELGQPADRIPWVVLRARDLADIERAVIVEEPSLDVADVSP
TRAQWCGQDKLEPILRDEAVRRGADIRFHTRLDSFAQDADGVDAVIVDRGTGARTAVRSR
YLIAADGVRSTVRQALGVTGTGHGSLGKAMSVLFQADFEPVLHGRRFVITYMANPQAPGV
LQTFDENRWIFGFFCDAYGGGDAAFDTGRCADIVRTSLGIPDIPLDVQLVQPWEMSHHVA
DSYRSGRVFLAGDAAHVHPPAGAFGANGGIQDAHNLAWKLASVLHGRASDALLDTYHQER
HPVGTEIAEQAWTRHTYRLDGDDELGRRLVDTKVVAAGYRYTSSAVLGAAYPTAIPHELA
LTGLPGQRVPHVWLDHDGRRVSTVDLAVDGFVLLARADGTPWADAAARLAATTGIPLTAH
VVGKTLTDPADALAAATGLGEAGALLLRPDGFVAWRSDTSADDPEAVLDGVLARILART
selected fasta
>hydroxylase [TcmB2 oxygenase]
ATGGACCGAATAGAAATTCCCGTACTCGTCGTGGGCGGCGGACTGACCGGACTGGCGGCG
GCCGTCTTCCTCCGCCAGCAGGGGGTCGACTGCCTCCTGGTGGAGCGTCACCGCAGCACC
ACCTTCCTGACCCGCGCCTCCGGGATCAACGCCCGCACCATGGAACTGCTGCGCAACGCC
GGTCTGGAGGAGACGGTGATCGACCGCAGCCTCCACCTCATCGAGGGCAAGCGCTGGCGT
GAACTCGGCCAGCCCGCCGACCGGATCCCCTGGGTCGTCCTGCGCGCCCGCGACCTGGCC
GACATCGAGCGGGCGGTGATCGTCGAGGAACCCTCCCTGGACGTGGCGGACGTCAGCCCC
ACCCGAGCGCAGTGGTGCGGCCAGGACAAACTCGAACCGATCCTCCGCGACGAGGCGGTG
CGCCGTGGCGCCGACATCCGCTTCCACACCCGGCTGGATTCCTTCGCCCAGGACGCGGAC
GGGGTCGACGCGGTCATCGTGGACCGCGGCACGGGCGCCCGCACCGCCGTGCGCAGCCGG
TACCTCATCGCCGCGGACGGGGTGCGCAGCACCGTCCGGCAGGCCCTCGGCGTCACCGGC
ACCGGCCACGGCTCGCTGGGCAAGGCGATGAGCGTCCTGTTCCAGGCCGACTTCGAACCG
GTCCTGCACGGCCGGCGCTTCGTCATCACCTACATGGCCAACCCGCAGGCGCCCGGCGTC
CTGCAGACCTTCGACGAGAACCGGTGGATCTTCGGGTTCTTCTGCGACGCGTACGGCGGC
GGCGACGCCGCGTTCGACACCGGGCGCTGCGCGGACATCGTGCGGACCTCCCTCGGCATC
CCCGACATCCCCCTGGACGTCCAGCTGGTCCAGCCCTGGGAGATGTCGCACCACGTCGCC
GACAGCTACCGCAGCGGACGGGTCTTCCTCGCCGGCGACGCCGCGCACGTCCATCCGCCC
GCGGGCGCCTTCGGGGCCAACGGCGGGATCCAGGACGCGCACAACCTGGCCTGGAAACTC
GCCTCCGTCCTGCACGGCCGGGCGAGCGACGCCCTCCTCGACACCTACCACCAGGAGCGG
CACCCGGTCGGCACGGAGATCGCCGAACAGGCCTGGACGCGCCACACGTACCGGCTCGAC
GGGGACGACGAGCTGGGGCGACGCCTCGTCGACACCAAGGTCGTGGCCGCCGGCTACCGC
TACACGTCCTCGGCCGTGCTGGGAGCGGCGTACCCCACGGCGATCCCGCACGAGCTGGCG
CTCACCGGCCTCCCGGGGCAGCGCGTCCCGCACGTGTGGCTCGACCACGACGGCCGCCGC
GTCTCGACCGTCGACCTCGCCGTGGACGGATTCGTCCTGCTCGCCCGGGCCGACGGCACA
CCGTGGGCGGACGCGGCGGCCCGGCTCGCCGCGACGACCGGCATCCCGCTCACCGCCCAT
GTCGTCGGCAAGACCCTGACCGACCCCGCCGACGCGCTCGCCGCCGCCACGGGACTCGGC
GAGGCGGGCGCCCTGCTGCTGCGCCCGGACGGCTTCGTCGCCTGGCGCAGCGACACGTCC
GCCGACGACCCCGAAGCCGTACTCGACGGCGTCCTCGCCCGGATCCTGGCCCGCACCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002938 Monooxygenase, FAD-binding (Domain)
[5-370] 2.99999999999998e-107 PF01494
PF01494   FAD_binding_3
IPR003042 Aromatic-ring hydroxylase-like (Domain)
[7-29] 1.49999648196323e-45 PR00420 [178-193] 1.49999648196323e-45 PR00420 [305-320] 1.49999648196323e-45 PR00420 [320-336] 1.49999648196323e-45 PR00420 [338-356] 1.49999648196323e-45 PR00420 [356-372] 1.49999648196323e-45 PR00420
PR00420   RNGMNOXGNASE
SignalP
[1-21] 0.432 Signal
Eukaryota   
TMHMM
[7-24] Transmembrane (i-o)
Transmembrane 1   
Ello_00060 Ello_00060   Ello_00080 Ello_00080
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