Ello_00070 Ello_00070   Ello_00090 Ello_00090

Ello_00080 : CDS information

close this sectionLocation

Organism
StrainTü2353
Entry nameElloramycin
Contig
Start / Stop / Direction7,813 / 8,142 / + [in whole cluster]
7,813 / 8,142 / + [in contig]
Location7813..8142 [in whole cluster]
7813..8142 [in contig]
TypeCDS
Length330 bp (109 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
ProductC-5 monooxygenase
Product (GenBank)TcmF1 monooxygenase
Gene
Gene (GenBank)elmH
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[11325225] Cloning, sequencing, and heterologous expression of the elmGHIJ genes involved in the biosynthesis of the polyketide antibiotic elloramycin from Streptomyces olivaceus Tu2353. (J Nat Prod. , 2001)
[18310024] Biosynthesis of elloramycin in Streptomyces olivaceus requires glycosylation by enzymes encoded outside the aglycon cluster. (Microbiology. , 2008)
comment
[PMID:11325225](2001)
Elloramycin A産生に関連するcosmid clone 16F4からの遺伝子同定、配列解析とその遺伝子の異種性発現。

ElmH: tetracenomycin F1 monooxygenase

E.coliでelmHを過剰発現、ElmH精製、活性確認。
tetracenomycin(Tcm) F1 → Tcm D3へのC-5 oxidation(quinone形成)を、TLCで基質変換をみることにより確認。

---
[PMID:18310024](2008)
elloramycin clusterの同定論文。
elmHに関しては上記論文をRefとして引用。

elmH: TcmF1 monooxygenase

close this sectionSequence

selected fasta
>C-5 monooxygenase [TcmF1 monooxygenase]
MPRLSPDAAYYTLVNVFTVSPDDQEKLYRHLVDVTERTIRHLPGFVSATFHLGNNGRHVV
NYAQWESEETFRSMHALPELQEHFAFCRGIATPLSVPCAVAQVFEEAAG
selected fasta
>C-5 monooxygenase [TcmF1 monooxygenase]
ATGCCCCGACTGTCCCCCGACGCCGCGTACTACACGCTCGTCAACGTCTTCACCGTCTCG
CCCGACGACCAGGAGAAGCTCTACCGCCACCTCGTCGACGTGACCGAGCGGACCATCCGC
CACCTCCCCGGTTTCGTCTCCGCCACCTTCCACCTGGGCAACAACGGCCGGCACGTCGTC
AACTACGCCCAGTGGGAGAGCGAGGAAACGTTTCGCTCCATGCATGCGCTGCCCGAGCTC
CAGGAGCACTTCGCCTTCTGCCGCGGCATCGCCACCCCCCTGTCCGTGCCCTGCGCCGTG
GCCCAGGTCTTCGAGGAGGCCGCCGGATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR007138 Antibiotic biosynthesis monooxygenase (Domain)
[11-84] 1e-16 PF03992
PF03992   ABM
IPR011008 Dimeric alpha-beta barrel (Domain)
[1-104] 6.79998707392904e-25 SSF54909
SSF54909   Dimer_A_B_barrel
SignalP No significant hit
TMHMM No significant hit
Ello_00070 Ello_00070   Ello_00090 Ello_00090
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