Ello_00170 : CDS information

Location

Organism	Streptomyces olivaceus
Strain	Tü2353
Entry name	Elloramycin
Contig	AM900040
Start / Stop / Direction	14,028 / 15,164 / + [in whole cluster] 14,028 / 15,164 / + [in contig]
Location	14028..15164 [in whole cluster] 14028..15164 [in contig]
Type	CDS
Length	1,137 bp (378 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	L-rhamnose C-2' O-methyltransferase
Product (GenBank)	rhamnose C2'-methyltransferase
Gene
Gene (GenBank)	elmMI
EC number	2.1.1.-
Keyword	tri-O-methylrhamnose
Note
Note (GenBank)
Reference	ACC Q9AJU2 PmId [11376004] Deoxysugar methylation during biosynthesis of the antitumor polyketide elloramycin by Streptomyces olivaceus. Characterization of three methyltransferase genes. (J Biol Chem. , 2001) [18310024] Biosynthesis of elloramycin in Streptomyces olivaceus requires glycosylation by enzymes encoded outside the aglycon cluster. (Microbiology. , 2008) comment [PMID:11376004](2001) elloramycin aglycone合成cosmid cos16F4からelmMI, elmMII, elmMIII を同定。配列解析、DB比較、各geneの異種性発現、vitroでのmethylating activity測定。これらの結果から、ElmMI はaglyconeに付着した後の糖の2'-positionでOH基のメチル化をする。各種糖基質に対する活性の違いから、C2', C3', C4'の順にメチル化されることもわかっている。 -- [PMID:18310024](2008) elloramycin cluster同定論文。 elmMI の機能に関しては上記論文引用。 elmMI : Rhamnose C2'-methyltransferase

Sequence

>L-rhamnose C-2' O-methyltransferase [rhamnose C2'-methyltransferase]
MDSPQVARTLVDSAGGTAAHTREAIRQIGVPETAAFLADELAGRTETVTIRHAAEVQFVF
DDRYAPDAADPVPWTFRVGPEGVTHRAGALPDPGAVVTQDLTELARSLYGPAADRSDATR
TVWWRDHDDPRVYFDPPPVFPAVERLLAAADGRDVPGLAGLALRHGSDKWGIHTYTAAYE
QHFAPFRDRAVTVVEIGVGGYDDPAAGGGSLRMWKRYFRRGLVYGVDIADKSRHREPRVH
TVVADQSDPASLRDLADAIGPIDIVIDDGSHISAHVVTAFSTLFPRLNPGGLYVVEDLQT
SYWPAFQGAYDDDTRTSVGFLKRLVDGLHHAEYPSRAGRPAQPTDRTVGSLHFHPNLAFV
EKRANSGHGGISRLREAT

>L-rhamnose C-2' O-methyltransferase [rhamnose C2'-methyltransferase]
ATGGACTCCCCACAGGTGGCACGGACCCTCGTCGACAGCGCGGGAGGCACCGCGGCGCAC
ACCCGTGAGGCCATCCGACAGATCGGCGTCCCGGAGACCGCCGCGTTCCTCGCCGACGAA
CTCGCCGGGCGCACGGAGACCGTGACGATCCGTCACGCGGCCGAGGTCCAGTTCGTGTTC
GACGACCGGTACGCGCCGGACGCGGCCGACCCGGTCCCCTGGACGTTCCGGGTCGGCCCG
GAGGGCGTCACCCACCGGGCCGGCGCGCTTCCGGACCCCGGGGCCGTGGTGACCCAGGAC
CTGACCGAACTGGCGCGCTCCCTGTACGGTCCGGCCGCCGACCGCTCCGACGCCACCCGC
ACGGTGTGGTGGCGCGACCACGACGATCCCCGCGTCTACTTCGACCCGCCCCCCGTGTTC
CCCGCCGTCGAACGCCTGCTGGCCGCCGCCGACGGACGGGACGTCCCCGGCCTGGCCGGA
CTCGCCCTGCGCCACGGCTCGGACAAGTGGGGGATCCACACGTACACGGCCGCCTACGAG
CAGCACTTCGCGCCGTTCCGGGACCGTGCCGTCACCGTCGTCGAGATCGGCGTCGGCGGA
TACGACGACCCCGCGGCGGGCGGCGGCTCCCTGCGCATGTGGAAGCGCTACTTCCGGCGC
GGGCTCGTCTACGGCGTGGACATCGCGGACAAGAGCAGGCACCGCGAGCCACGGGTGCAC
ACCGTCGTCGCGGACCAGTCCGACCCCGCCTCCCTGCGCGACCTGGCCGACGCGATCGGC
CCCATCGACATCGTCATCGACGACGGCAGCCACATCAGCGCCCATGTGGTGACCGCCTTC
TCCACCCTGTTCCCCCGGCTCAACCCCGGCGGGCTGTACGTGGTCGAGGACCTGCAGACC
TCGTACTGGCCGGCCTTCCAGGGCGCGTACGACGACGACACCCGCACCAGCGTCGGCTTC
CTCAAGCGGCTCGTCGACGGCCTGCACCACGCCGAGTACCCGTCCCGGGCCGGGCGCCCC
GCCCAGCCGACCGACCGAACCGTCGGCAGCCTGCACTTCCACCCCAACCTGGCCTTCGTG
GAGAAACGTGCCAACAGCGGCCACGGCGGCATCAGCCGGCTGCGGGAAGCGACCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	No significant hit
TMHMM	No significant hit

Ello_00160 Ello_00180