Ello_00150 Ello_00150   Ello_00170 Ello_00170

Ello_00160 : CDS information

close this sectionLocation

Organism
StrainTü2353
Entry nameElloramycin
Contig
Start / Stop / Direction13,178 / 14,008 / + [in whole cluster]
13,178 / 14,008 / + [in contig]
Location13178..14008 [in whole cluster]
13178..14008 [in contig]
TypeCDS
Length831 bp (276 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.2 modification methylation
Productputative C-9 carboxyl O-methyltransferase
Product (GenBank)carboxyl O-methyltransferase
Gene
Gene (GenBank)elmP
EC number2.1.1.-
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[18310024] Biosynthesis of elloramycin in Streptomyces olivaceus requires glycosylation by enzymes encoded outside the aglycon cluster. (Microbiology. , 2008)
comment
elloramycin clusterの同定論文。
elmPについては配列解析のみ。

elmP: Carboxyl O-methyltransferase

closest matchはTcmP.
中間体tetracenomycin B3のC-9に付着したcarboxyl基で働くO-methyltransferaseであると提唱されている。

tetracenomycin B3 → tetracenomycin B2 (9-carboxymethyl-tetracenomycin B3)
Related Reference
ACC
P39887
PmId
[8509339] ( , )
comment
Blast 2nd, id60%, 2e-86
Streptomyces glaucescens_tcmP
Tetracenomycin polyketide synthesis O-methyltransferase tcmP

S.glaucescensのtetracenomycin(TCM) C合成に関わる遺伝子群のうち、tcmGおよびtcmPについての解析。クローニング、シークエンス解析およびrecombinant(E.coliにて過剰発現)を用いた機能解析。

・tcmPはO-methyltransferaseをコードし、 TCM EのC-9 carboxy基をメチル化してTCM A2を得る。
・tcmGはTCM A2のpositions C-4, C-4a, C-12aでのhydroxylationを担い、TCM Cをもたらす。

これら2遺伝子産物は、TCM C合成における最終段階で利用される。

close this sectionSequence

selected fasta
>putative C-9 carboxyl O-methyltransferase [carboxyl O-methyltransferase]
MSPDPARVAAVRLTGVQETLLAPLYVRALDNRAPRPVLGDRMAEPLMRRLDYDFRKMRFA
GRDRRTVPFRARKLDEWTAAFLGAHPDAVVVHLACGLDTRAQRLDVPRTALWFDVDLPDV
TALRRELFEESDGYRMLAGSVVETDWLGEVPRDRPALVVAEGLAPYLAEDDGLTMLRNLT
AHLSGGEILFDAVLPWTRHIARYARLIRQTGASFGWAVADPRDLERQVPGLRALDEWALC
DSPYADRLPPRDRLVLGAMRAVRPLRYAYRLLRYRF
selected fasta
>putative C-9 carboxyl O-methyltransferase [carboxyl O-methyltransferase]
ATGAGCCCGGACCCGGCGCGCGTCGCCGCCGTCCGGCTGACCGGAGTGCAGGAGACCCTG
CTCGCCCCCCTGTACGTCCGTGCCCTCGACAACCGCGCACCGCGCCCCGTGCTCGGCGAC
CGCATGGCCGAACCGCTGATGCGCCGCCTGGACTACGACTTCCGGAAGATGCGCTTCGCC
GGTCGCGACCGGCGCACGGTCCCCTTCCGGGCCCGCAAGCTCGACGAGTGGACGGCCGCC
TTCCTCGGCGCCCACCCGGACGCCGTGGTCGTGCACCTGGCGTGCGGCCTCGACACCCGG
GCCCAGCGGCTCGACGTACCGCGCACCGCGCTCTGGTTCGACGTCGACCTGCCCGACGTG
ACGGCACTGCGCCGGGAGCTGTTCGAGGAGTCCGACGGCTACCGGATGCTCGCCGGCTCC
GTGGTCGAGACGGACTGGCTGGGCGAGGTGCCGCGCGACCGGCCGGCACTGGTGGTCGCC
GAGGGGCTCGCCCCGTACCTCGCCGAGGACGACGGCCTCACCATGCTGCGCAACCTCACG
GCCCATCTGTCGGGCGGCGAGATCCTCTTCGACGCCGTGCTGCCCTGGACCCGTCACATC
GCCCGGTACGCGCGCCTCATCCGGCAGACCGGCGCGTCCTTCGGCTGGGCGGTCGCCGAC
CCCCGAGACCTGGAGCGGCAGGTCCCCGGTCTGCGGGCGCTCGACGAGTGGGCCCTGTGC
GACTCGCCGTACGCCGACCGGCTCCCGCCGCGCGACCGGCTGGTCCTGGGCGCCATGCGC
GCGGTGCGGCCCCTGCGCTACGCCTACCGGCTGCTGCGCTACCGATTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR007213 Leucine carboxyl methyltransferase (Family)
[21-179] 6.19999999999994e-40 PF04072
PF04072   LCM
IPR016874 Polyketide synthesis O-methyltransferase, TcmP (Family)
[1-276] 3.99998544139379e-99 PIRSF028177
PIRSF028177   Polyketide_synth_Omtfrase_TcmP
SignalP
[1-28] 0.153 Signal
Bacteria, Gram-positive   
[1-28] 0.259 Signal
Eukaryota   
[1-28] 0.212 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Ello_00150 Ello_00150   Ello_00170 Ello_00170
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