Ello_00190 : CDS information

Location

Organism	Streptomyces olivaceus
Strain	Tü2353
Entry name	Elloramycin
Contig	AM900040
Start / Stop / Direction	16,311 / 17,483 / + [in whole cluster] 16,311 / 17,483 / + [in contig]
Location	16311..17483 [in whole cluster] 16311..17483 [in contig]
Type	CDS
Length	1,173 bp (390 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	L-rhamnose C-3' O-methyltransferase
Product (GenBank)	rhamnose C3'-methyltransferase
Gene
Gene (GenBank)	elmMII
EC number	2.1.1.-
Keyword	tri-O-methylrhamnose
Note
Note (GenBank)
Reference	ACC Q9AJU1 PmId [11376004] Deoxysugar methylation during biosynthesis of the antitumor polyketide elloramycin by Streptomyces olivaceus. Characterization of three methyltransferase genes. (J Biol Chem. , 2001) [18310024] Biosynthesis of elloramycin in Streptomyces olivaceus requires glycosylation by enzymes encoded outside the aglycon cluster. (Microbiology. , 2008) comment [PMID:11376004](2001) elloramycin aglycone合成cosmid cos16F4からelmMI, elmMII, and elmMIII を同定。配列解析、DB比較、各geneの異種性発現、vitroでのmethylating activity測定。これらの結果から、ElmMII はaglyconeに付着した後の糖の3'-positionでOH基のメチル化をする。各種糖基質に対する活性の違いから、C2', C3', C4'の順にメチル化されることもわかっている。 -- [PMID:18310024](2008) elloramycin cluster同定論文。 elmMII の機能に関しては上記論文引用。 elmMII : Rhamnose C3'-methyltransferase

Sequence

>L-rhamnose C-3' O-methyltransferase [rhamnose C3'-methyltransferase]
MTTPSPTPPLAAAEVAAAAGLQQRDLLAVLDRVGLEPAVAFLVHDLTARCDTPDNPDAAR
IGLAVEHSGHRTERVLAVRKGEPVRVDEETAGPPPVRLTFDLADLVRGVYGPPPGPGAGL
FRVERDDAWFVKNADDAEPFRIFESYMRAVDVLVRAATSRPGDLGRLAARHASDKWGLWH
WFTPLYEHHFARLRHQPVRVLELGIGGYQNPDEGGGSLKMWRSYFPQGRIFGVDYFPKHG
LDEDRIHTLQGSQDDAGFLRRVAEEHGPFDIVIDDGSHVAGHQQTAFRTLFPAVRNGGFY
VIEDLWTAYCPGYGGAATARAEGRTSIGLLKSLLDDLHYEEWTAPEPAAPGFAAPSLVGV
HVYRNLAVLEKGRNSEGTIPFFAPREIDYV

>L-rhamnose C-3' O-methyltransferase [rhamnose C3'-methyltransferase]
ATGACCACCCCTTCACCCACGCCACCGCTCGCGGCCGCCGAGGTGGCCGCCGCCGCGGGA
CTCCAGCAGCGGGACCTGCTCGCGGTGCTCGACCGGGTCGGCCTGGAACCCGCCGTCGCG
TTCCTCGTCCATGACCTCACGGCCCGCTGCGACACCCCCGACAACCCGGACGCGGCCCGG
ATCGGCCTCGCCGTCGAGCACAGCGGACACCGGACCGAGCGGGTCCTCGCCGTGCGCAAG
GGCGAGCCCGTCCGGGTGGACGAGGAGACGGCCGGTCCGCCTCCCGTACGTCTGACCTTC
GACCTCGCCGACCTGGTCCGTGGCGTGTACGGACCGCCGCCCGGTCCCGGAGCCGGTCTG
TTCCGGGTCGAACGCGACGACGCCTGGTTCGTCAAGAACGCCGACGACGCCGAGCCCTTC
CGGATCTTCGAGTCGTACATGCGCGCCGTCGACGTCCTCGTCCGCGCCGCCACGTCCCGG
CCCGGCGACCTGGGGCGGCTCGCGGCGCGCCACGCCTCCGACAAGTGGGGCCTGTGGCAC
TGGTTCACGCCCCTGTACGAACACCACTTCGCCCGGCTGCGCCACCAGCCGGTCCGGGTC
CTCGAACTCGGCATCGGCGGCTACCAGAACCCGGACGAGGGCGGCGGCTCGCTGAAGATG
TGGCGGTCCTACTTCCCGCAGGGCCGGATCTTCGGCGTCGACTACTTCCCGAAGCACGGC
CTGGACGAGGACCGCATCCACACCCTCCAGGGCAGCCAGGACGACGCCGGATTCCTGCGC
CGGGTCGCCGAGGAGCACGGGCCGTTCGACATCGTGATCGACGACGGCAGCCATGTGGCC
GGGCACCAGCAGACGGCGTTCCGCACGCTCTTCCCGGCGGTCCGCAACGGGGGCTTCTAC
GTCATCGAGGACCTGTGGACGGCGTACTGCCCCGGCTACGGGGGTGCCGCGACCGCACGC
GCGGAGGGCCGGACCTCCATCGGACTGCTCAAGTCCCTGCTGGACGACCTGCATTACGAG
GAGTGGACCGCGCCGGAGCCGGCCGCCCCCGGATTCGCCGCCCCGTCCCTGGTCGGCGTC
CACGTCTACCGCAACCTCGCGGTCCTGGAGAAGGGCCGCAACAGCGAGGGAACGATCCCG
TTCTTCGCGCCCCGAGAGATCGACTACGTATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	No significant hit
TMHMM	No significant hit

Ello_00180 Ello_00200