Ello_00190 Ello_00190   Ello_00210 Ello_00210

Ello_00200 : CDS information

close this sectionLocation

Organism
StrainTü2353
Entry nameElloramycin
Contig
Start / Stop / Direction17,510 / 18,295 / + [in whole cluster]
17,510 / 18,295 / + [in contig]
Location17510..18295 [in whole cluster]
17510..18295 [in contig]
TypeCDS
Length786 bp (261 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductL-rhamnose C-4' O-methyltransferase
Product (GenBank)rhamnose C4'-methyltransferase
Gene
Gene (GenBank)elmMIII
EC number2.1.1.-
Keyword
  • tri-O-methylrhamnose
Note
Note (GenBank)
Reference
ACC
PmId
[11376004] Deoxysugar methylation during biosynthesis of the antitumor polyketide elloramycin by Streptomyces olivaceus. Characterization of three methyltransferase genes. (J Biol Chem. , 2001)
[18310024] Biosynthesis of elloramycin in Streptomyces olivaceus requires glycosylation by enzymes encoded outside the aglycon cluster. (Microbiology. , 2008)
comment
[PMID:11376004](2001)
elloramycin aglycone合成cosmid cos16F4からelmMI, elmMII, and elmMIII を同定。
配列解析、DB比較、各geneの異種性発現、vitroでのmethylating activity測定。

これらの結果から、ElmMIII はaglyconeに付着した後の糖の4'-positionでOH基のメチル化をする。
各種糖基質に対する活性の違いから、C2', C3', C4'の順にメチル化されることもわかっている。

--
[PMID:18310024](2008)
elloramycin cluster同定論文。
elmMIII の機能に関しては上記論文引用。

elmMIII : Rhamnose C4'-methyltransferase

close this sectionSequence

selected fasta
>L-rhamnose C-4' O-methyltransferase [rhamnose C4'-methyltransferase]
MTEDARDLYLDLMKKVLTNLIYRDAPIQTFVYDGEPDADPRLLGRDWPSVAHTMVGLKRL
DNLQYCVETVLADGVPGDLVETGVWRGGSSIFMRAVLRAHGDTARRVWVADSFEGMPEVG
ADSHAVDREMRLHEHNGVLAVPLEQVRANFERYGLLDDQVRFLPGWFKDTLPGAPTGRLA
VIRLDGDLYESTTDALENLMPRLSPGGFVIIDDYAIDACRDAVHDYRGRYGISDPISEID
GTGVFWRHTAASARSLQPATV
selected fasta
>L-rhamnose C-4' O-methyltransferase [rhamnose C4'-methyltransferase]
ATGACGGAAGACGCCCGCGACCTGTACCTCGACCTGATGAAGAAGGTGCTGACCAACCTG
ATCTACCGGGACGCGCCGATCCAGACGTTCGTCTACGACGGGGAACCCGACGCCGACCCC
CGGCTCCTCGGCCGCGACTGGCCGAGCGTCGCCCACACCATGGTCGGGCTCAAGCGGCTG
GACAACCTGCAGTACTGCGTCGAGACGGTGCTGGCCGACGGGGTGCCGGGGGACCTGGTG
GAGACCGGCGTCTGGCGCGGCGGTTCCTCGATCTTCATGCGGGCCGTGCTCCGCGCCCAC
GGCGACACCGCGCGCCGCGTCTGGGTCGCCGACTCCTTCGAGGGCATGCCGGAGGTGGGC
GCGGACAGCCACGCCGTCGACCGGGAGATGCGCCTCCACGAACACAACGGCGTCCTGGCG
GTGCCACTGGAGCAGGTGCGGGCCAACTTCGAGCGCTACGGCCTGCTCGACGACCAGGTG
CGGTTCCTGCCCGGCTGGTTCAAGGACACGCTGCCCGGCGCGCCGACGGGCCGGCTGGCG
GTCATCAGGCTGGACGGCGACCTGTACGAGTCGACCACGGACGCGTTGGAGAACCTGATG
CCGAGGCTCTCCCCCGGCGGCTTCGTCATCATCGACGACTACGCCATCGACGCCTGCCGG
GACGCGGTGCACGACTACCGCGGCCGGTACGGGATCTCCGACCCGATCAGCGAGATCGAC
GGCACCGGCGTCTTCTGGCGCCACACGGCCGCGTCCGCCCGCAGCCTCCAGCCGGCCACC
GTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR008884 Macrocin-O-methyltransferase (Family)
[3-248] 2.50000000000001e-126 PF05711
PF05711   TylF
SignalP No significant hit
TMHMM No significant hit
Ello_00190 Ello_00190   Ello_00210 Ello_00210
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