Fren_00010 : CDS information

Location

Organism	Streptomyces roseofulvus
Strain	AK24158
Entry name	Frenolicin
Contig	AF058302
Start / Stop / Direction	1,563 / 3,875 / + [in whole cluster] 1,563 / 3,875 / + [in contig]
Location	1563..3875 [in whole cluster] 1563..3875 [in contig]
Type	CDS
Length	2,313 bp (770 aa)

Annotation

Category	4.3 transport
Product	putative transcriptional regulator/putative ABC transporter substrate-binding protein
Product (GenBank)	FrnA
Gene
Gene (GenBank)	frnA
EC number
Keyword
Note	bifunctional protein
Note (GenBank)	similar to Escherichia coli nikA
Reference	ACC O68907 comment "Analysis of a 27 kb region of Streptomyces roseofulvus containing genes for frenolicin biosynthesis." 論文として報告があるのは10.2kbp領域のみ[PMID:8181754]。このORFはその範囲外でGenBank登録AF058302に由来する。
Related Reference	ACC Q9AL82 PmId [11133934] Identification of the nik gene cluster of Brucella suis: regulation and contribution to urease activity. (J Bacteriol. , 2001) comment 364th(Q9AL82) 27%, 3e-28, C末側hit Brucella suis_nikA Nickel ABC transporter, nickel-binding protein Brucella suisでのNickel transporterの解析。 NikオペロンはE.coliに非常によく似ていて E.coliのNik deficientと相補可能。 NikAはNiCLの存在下で発現は促進される。またUrease（Nickelを取り込んだ後の代謝酵素）の活性が NikA deficientだと弱い。（促進されない）
Family/Domain	FD IPR004474 comment N-term [IPR004474] Cell envelope-related transcriptional attenuator (Domain)
Family/Domain	FD IPR000914 comment C-term [IPR000914] Bacterial extracellular solute-binding protein, family 5 (Family) function; transporter activity

Sequence

>putative transcriptional regulator/putative ABC transporter substrate-binding protein [FrnA]
MAGSGYAYYQHLSGNIDKIDVGDAGNKDAAPDGPINILIIGTDKRTGKGNEGCGGKDSPG
HADTNILLRVSADRTNTTGLSIPRDLITNIPDCLTTQDDGSKKTIPGTQNVRFNTSLGQE
GRDPGCTMRTVTELTGLKVDHFMMADFNAVKNLTTAVNGVEVCVAKDVDDPDSHLKLSAG
THKVQGEQALAFVRTRHSFGNQGDLDRIKVQQQFLGSLARQLKSEDTLTSPKKLYKVAEA
ATDALTVDSGIGSITKLMSLAKELQHINPKNITFVTLPVVDNPAEKVKATVVLNETDADP
QQSALGQSLDVGRQLVDSLTDQDPRDGKTVPWLATRWKADPEATRFTFTLRAGATFSDGT
PVDARAVKANFDAVHELGAAASRGAVYLDGYRETRVSGARTLTVVFDKPNAQFLRGTSTV
SLGLLSPGSLRRTPQERCTGRLVGSGPFVLDRYRPNTSVTLDRRKGYSWGSRLWQREGGA
YLEGVEYRIVPENTTRSGALSAGQLDVATALAPQDRERFSAPGWSLLTRTAPGVDLSLYV
NARRTALREAAVRQALQKGIDREAVATTFLSSRKLAATSVLSSTTPGYTDLGDRLAHDPA
GARRLLDKAGWRPGADGIRVKNGVRLRLDAVFVRQQSLELVQQQLKDIGVELRLRQLTVS
RFPEVLAAGSYDLSLQSANRADPDVLTTAFAGGTPVADARLRSELRRATSSTDEATRSSL
FAAAQRRLVDEGHVLPLNETEETAALSTRVHGLTRDASNRLVLHDTWTTG

>putative transcriptional regulator/putative ABC transporter substrate-binding protein [FrnA]
GTGGCCGGCAGCGGCTACGCGTACTACCAGCACCTCAGCGGCAACATCGACAAGATTGAC
GTCGGCGACGCGGGCAACAAGGACGCCGCCCCCGACGGGCCGATCAACATCCTGATCATC
GGCACCGACAAGCGCACCGGCAAGGGCAACGAGGGGTGCGGGGGCAAGGACAGCCCTGGC
CACGCGGACACCAACATCTTGCTGCGCGTCTCCGCCGACCGCACCAACACGACCGGCCTG
AGCATTCCGCGTGACCTCATCACGAACATCCCGGACTGCCTGACCACCCAGGACGACGGT
TCGAAGAAGACGATCCCCGGCACGCAGAACGTCCGCTTCAACACGAGCCTCGGGCAGGAG
GGCCGCGACCCCGGCTGCACGATGCGCACGGTCACCGAGCTGACCGGGCTCAAGGTCGAC
CACTTCATGATGGCCGACTTCAACGCGGTCAAGAACCTGACGACGGCCGTGAACGGCGTC
GAGGTGTGCGTGGCGAAGGACGTCGACGACCCCGACTCGCACCTCAAGCTCTCGGCGGGG
ACGCACAAGGTGCAGGGCGAGCAGGCGCTCGCCTTCGTCCGCACCCGGCACAGCTTCGGC
AACCAGGGCGACCTCGACCGCATCAAGGTCCAGCAGCAGTTCCTCGGTTCGCTCGCGCGG
CAGCTCAAGTCCGAGGACACGCTGACGAGTCCGAAGAAGCTCTACAAGGTCGCCGAGGCG
GCGACCGACGCGCTGACCGTGGACTCGGGGATCGGCAGCATCACGAAACTGATGTCGCTG
GCCAAGGAGTTGCAGCACATCAATCCGAAGAACATCACCTTCGTGACGCTCCCCGTGGTC
GACAACCCGGCCGAGAAGGTCAAGGCGACGGTCGTCCTCAATGAGACGGACGCTGATCCG
CAGCAGTCGGCTCTCGGGCAGAGTCTCGACGTGGGCCGCCAGCTCGTCGACTCGCTCACC
GACCAGGACCCGCGCGACGGCAAGACCGTCCCGTGGCTCGCGACGCGCTGGAAGGCCGAC
CCCGAGGCCACCCGCTTCACCTTCACCCTGCGCGCGGGCGCCACTTTCAGCGACGGCACA
CCGGTCGACGCGCGCGCGGTCAAGGCCAACTTCGACGCCGTGCACGAGCTCGGCGCCGCC
GCCTCGCGGGGGGCCGTGTACCTCGACGGCTACCGCGAGACGCGCGTCAGCGGCGCCCGT
ACGCTCACGGTCGTCTTCGACAAGCCGAACGCCCAGTTCCTGCGCGGCACGTCCACCGTC
TCACTGGGCCTGCTCTCGCCGGGCAGCCTGCGCCGCACCCCGCAGGAGCGCTGCACCGGG
CGCCTCGTCGGCTCCGGTCCCTTCGTGCTCGACCGCTACCGGCCGAACACCTCCGTCACG
CTGGACCGGAGAAAGGGCTACTCCTGGGGCTCGCGCCTGTGGCAACGGGAGGGCGGCGCG
TACCTGGAGGGGGTCGAGTACCGGATCGTGCCCGAGAACACGACCCGCTCGGGCGCCCTG
TCCGCCGGTCAGCTCGACGTCGCCACCGCGCTCGCGCCGCAGGACAGGGAGCGGTTCTCC
GCCCCCGGCTGGTCGCTGCTGACCCGTACCGCGCCCGGCGTCGACCTCAGCCTGTACGTC
AACGCGCGCCGCACGGCGCTGCGCGAGGCGGCGGTGCGCCAGGCCCTCCAGAAGGGCATC
GACCGCGAAGCCGTCGCCACGACCTTCCTCAGTTCCCGCAAGCTCGCGGCGACCAGCGTG
CTCTCCTCCACGACCCCCGGCTACACCGACCTCGGCGACCGCCTCGCCCACGACCCTGCG
GGTGCGAGGCGGCTGCTCGACAAGGCCGGATGGCGGCCCGGGGCGGACGGCATCAGGGTC
AAGAACGGCGTCAGGCTGCGACTCGACGCCGTCTTCGTCCGGCAGCAGAGCCTCGAACTC
GTGCAGCAGCAGCTCAAGGACATCGGCGTCGAACTGCGGCTGAGACAGCTCACCGTCTCC
CGTTTCCCCGAAGTGCTCGCCGCGGGCAGCTACGACCTGAGCCTCCAGAGCGCCAACCGC
GCCGATCCCGACGTCCTCACCACGGCCTTCGCGGGCGGCACCCCCGTCGCCGACGCGCGC
CTGCGCTCCGAACTGCGCCGGGCCACCTCCTCCACCGACGAGGCGACGCGCTCCTCGCTC
TTCGCCGCGGCGCAACGGCGTCTCGTCGACGAGGGGCACGTCCTGCCGCTCAACGAGACC
GAGGAGACCGCCGCGCTCTCGACGCGGGTGCACGGGCTGACGCGGGACGCCTCCAACCGG
CTGGTCCTGCACGACACCTGGACCACCGGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000914 Bacterial extracellular solute-binding protein, family 5 (Domain) PF00496 SBP_bac_5 IPR004474 Cell envelope-related transcriptional attenuator (Domain) TIGR00350 LytR_cpsA_psr PF03816 LytR_cpsA_psr
SignalP	No significant hit
TMHMM	No significant hit

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