Fren_00020 : CDS information

Location

Organism	Streptomyces roseofulvus
Strain	AK24158
Entry name	Frenolicin
Contig	AF058302
Start / Stop / Direction	3,872 / 4,843 / + [in whole cluster] 3,872 / 4,843 / + [in contig]
Location	3872..4843 [in whole cluster] 3872..4843 [in contig]
Type	CDS
Length	972 bp (323 aa)

Annotation

Category	4.3 transport
Product	putative ABC transporter permease protein
Product (GenBank)	membrane protein of putative ABC transporter
Gene
Gene (GenBank)	frnB
EC number
Keyword
Note
Note (GenBank)	FrnB; similar to Escherichia coli DPPB
Reference	ACC O68908 comment "Analysis of a 27 kb region of Streptomyces roseofulvus containing genes for frenolicin biosynthesis." 論文として報告があるのは10.2kbp領域のみ[PMID:8181754]。このORFはその範囲外でGenBank登録AF058302に由来する。
Related Reference	ACC O50262 PmId [9987134] Mannopinic acid and agropinic acid catabolism region of the octopine-type Ti plasmid pTi15955. (Mol Microbiol. , 1999) comment 400th(O50262) 33%, 3e-37 Rhizobium radiobacter_agaB AgaB AgaC, AgaB and AgaDはagropinic acid uptake systemに関与。 AgrobacteriumのAgaBがヒット。 Agrobacteriumが寄生した植物に作らせるアミノ酸誘導体でOpineにMannosyl基がついているもののTransporterを、Ti-plasmidのクローニングにより発見した。 Agropinic acid依存の発現を確認。
Related Reference	ACC P0AEF8 PmId [7536291] The dipeptide permease of Escherichia coli closely resembles other bacterial transport systems and shows growth-phase-dependent expression. (Mol Microbiol. , 1994) [16905647] The housekeeping dipeptide permease is the Escherichia coli heme transporter and functions with two optional peptide binding proteins. (Proc Natl Acad Sci U S A. , 2006) comment Blast id30.45%, 2e-23 E.coli_dppB Dipeptide transport system permease protein DppB --- [PMID:7536291](1994) abstract ２つまたは３つのL-アミノ酸から成るpeptideをtransportするdipeptide permease(Dpp)をコードするdpp locusのシークエンス。 dppABCDEのoperonを編成。 -- [PMID:16905647](2006) E.coli K12はヘム鉄を使用するため、dipeptide inner membrane ATP-binding cassette transporter (DppBCDF)と、2つのperiplasmic binding proteins: MppA or DppAのどちらかを要求する。

Sequence

>putative ABC transporter permease protein [membrane protein of putative ABC transporter]
MTARYLARRLGRVVLVVWAAYTLSFAVLYLLPGDPVQTMLSGAAGGDGAAVDPHEAQRLR
HTLGLDRPLAVQYTSMLGHALRGDLGTSIRSGAPVRGQLAQALPDTLSVALPALVLSVLV
ALCLALLGAWPRRRALRRAATALPSLGTAMPSFWLGLLLAQWVSFRWGLLPATGGGRSPR
ATLLAALTLALPIGCVLAQVLGRGLRAALAEPYADVARSRGAGRARLLLARALRNASVAA
LALLGVVCGQLLAGAVLVETVFARGGIGRLAMDAVTYQDLPVVQGVVVLAALVAALVNLV
VDLLLPLLEPRTASEAADAVPAH

>putative ABC transporter permease protein [membrane protein of putative ABC transporter]
GTGACGGCCCGCTACCTGGCCCGCCGGCTGGGCCGCGTCGTCCTCGTCGTCTGGGCCGCG
TACACCCTGTCCTTCGCCGTCCTCTACCTGCTGCCGGGAGATCCCGTGCAGACCATGCTG
AGCGGCGCGGCGGGCGGGGACGGGGCGGCCGTCGACCCGCACGAGGCCCAGCGGCTGCGC
CACACGCTCGGCCTCGACCGCCCGCTCGCCGTCCAGTACACCAGCATGCTGGGGCACGCC
CTGCGCGGAGACCTCGGCACCTCGATCCGCAGCGGGGCGCCCGTACGCGGGCAACTCGCC
CAAGCGCTGCCCGACACCCTCTCGGTGGCCCTCCCCGCGCTCGTCCTCAGCGTGCTCGTC
GCGCTCTGCCTCGCCCTCCTCGGGGCCTGGCCCCGGCGACGAGCCCTGCGCAGGGCGGCG
ACCGCCCTGCCCTCGCTCGGCACCGCGATGCCCAGCTTCTGGCTGGGGCTGCTCCTCGCC
CAGTGGGTCTCCTTCCGCTGGGGACTGCTCCCCGCCACGGGCGGCGGGCGCTCGCCCCGG
GCCACCCTGCTGGCCGCGCTGACCCTCGCCCTGCCCATCGGCTGCGTGCTCGCCCAGGTG
CTGGGGAGGGGCCTGCGCGCCGCGCTCGCCGAGCCGTACGCGGACGTGGCGCGCTCGCGC
GGCGCGGGGCGGGCCAGGCTGCTGCTCGCGCGCGCGCTGCGCAACGCCTCGGTGGCCGCC
CTGGCCCTGCTCGGCGTCGTGTGCGGGCAACTCCTCGCCGGGGCCGTCCTGGTGGAGACC
GTCTTCGCCAGGGGCGGCATCGGGCGCCTCGCCATGGACGCCGTCACCTACCAGGACCTG
CCCGTCGTGCAGGGCGTGGTGGTGCTCGCCGCCCTCGTCGCGGCGCTGGTCAACCTCGTG
GTGGACCTCCTCCTCCCGCTCCTCGAGCCGCGTACCGCCTCGGAGGCCGCCGATGCCGTC
CCTGCCCACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000515 Binding-protein-dependent transport systems inner membrane component (Family) PF00528 BPD_transp_1 PS50928 ABC_TM1
SignalP	Bacteria, Gram-positive Bacteria, Gram-negative Eukaryota
TMHMM	Transmembrane 6

Fren_00010 Fren_00030