Gelda_00080 : CDS information

Location

Organism	Streptomyces hygroscopicus
Strain	17997
Entry name	Geldanamycin
Contig	DQ914285
Start / Stop / Direction	26,954 / 26,088 / - [in whole cluster] 17,554 / 16,688 / - [in contig]
Location	complement(26088..26954) [in whole cluster] complement(16688..17554) [in contig]
Type	CDS
Length	867 bp (288 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative oxidoreductase
Product (GenBank)	GdmK
Gene
Gene (GenBank)	gdmK
EC number
Keyword	methoxymalonyl-ACP
Note
Note (GenBank)	methoxymalonyl-ACP biosynthesis pathway protein; fkbK
Reference	ACC Q06BL7 PmId [18214443] The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997. (Arch Microbiol. , 2008) comment geldanamycin生合成clusterにあるpositive regulators GdmRI and GdmRII に関する報告。 cluster schemeあり。 H, I, J, K, G(gdmH, I, J, K, G) : Methoxymalonyl-ACP biosynthetic genes 本文での詳細記載なし。
Related Reference	ACC Q9KIE3 NITE Asco_00110 PmId [15179529] Engineered biosynthesis of 16-membered macrolides that require methoxymalonyl-ACP precursors in Streptomyces fradiae. (Appl Microbiol Biotechnol. , 2004) comment Blast id64%, 9e-84 Streptomyces hygroscopicus subsp. ascomyceticus_fkbK FkbK S.hygroscopicus由来fkbG-Kを導入することにより、S. fradiaeはmalonyl-CoA, methylmalonyl-CoA, ethylmalonyl-CoAに加えてmethoxymalonyl-ACPもPKS前駆体として利用できるようになることを、midecamycin PKS genes（最後から2番目の縮合ステップでmethoxymalonateを要求）の異種性発現産物で確認している。
	ACC Q8KUG1 NITE Ansam_00150 PmId [11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002) comment Blast id63%, 7e-80 Actinosynnema pretiosum subsp. auranticum_asm13 3-hydroxyacyl-CoA dehydrogenase S. lividansで、AT domainsのひとつがmethoxymalonateに特異的なATで置換されているerythromycin PKSのカセットとasm13-17を同時発現すると、予測された位置にmethoxymalonateが取り込まれた6- deoxyerythronolide Bの派生体が産生された。よって、asm13-17はmethoxymalonyl-ACP生合成に十分である。
	ACC Q7BQ74 PmId [16983083] Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units. (Proc Natl Acad Sci U S A. , 2006) comment Blast id37%, 8e-46 Bacillus cereus_zmaG ZmaG ZmaGは、珍しいPKS伸長units, hydroxymalonyl-ACP and aminomalonyl-ACPの生合成に共通して関連する。 ZmaE or ZmaIと共に働く。 ZmaI: eryl-ZmaH(ACP)→aminomalonyl-ZmaH(ACP) ZmaE: glyceryl-ZmaD(ACP)→hydroxymalonyl-ZmaD(ACP)
	ACC Q9ADL9 NITE Soraph_00040 PmId [12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002) [15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004) comment Blast id40%, 5e-38 Sorangium cellulosum_sorD Beta-hydroxybutyryl-CoA dehydrogenase --- [PMID: 12039053](2002) Soraphen A biosynthetic gene clusterの報告。 SorD(295aa): beta-Hydroxybutyryl-CoA dehydrogenase Module 3,7で取り込まれるMethoxymalonyl-CoAを合成する。本ORFはCH2OHをCHOにする。 --- [PMID: 15289572](2004) sorC破壊株はsoraphen A非産生。 S. lividansでのsor genes異種性発現。 SorABRCDFE株 + cinnamate SorABRCDFE株 + badA(benzoate-CoA ligase) + benzoate or cinnamate の組み合わせでSoraphen A産生。 SorABRCDFE株で発現されているのは、the sorA, and the sorB4M, sorRCDFE operons.
	ACC P52041 PmId [8655474] Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. (J Bacteriol. , 1996) comment Blast id34%, 1e-37 Clostridium acetobutylicum_hbd 3-hydroxybutyryl-CoA dehydrogenase beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase (BHBD)の遺伝子のクローニングを行い、E. coliで過剰発現させたところBHBDの細胞外への分泌を確認。また、C. acetobutylicumで発現させ、BHBD activityの上昇を確認している。
	ACC Q84G15 NITE Gelda2_00170 PmId [12586396] Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602. (FEMS Microbiol Lett. , 2003) comment Streptomyces geldanamycininus NRRL 3602でのgeldanamycin生合成clusterにある対応遺伝子。

Sequence

>putative oxidoreductase [GdmK]
MSGHAEAHRLMVLGAGTMGLGITSLAVGHGIPVTVVEVDEAKAGRTRAAVTERLRMAQLM
RALPADRPPGELTVTASPADGREATAVVEAVTEDTPTKAKVLEAVAGLTGGRVPLISNTS
SIPIDELAGFIADPARLVGTHFMNPPYLIPTVEVIRGPRTGEAAMAAVTDLLRALERKPV
VVGDGPGFVTSRVLHPMINDAIRVVQEGTATAEAVDVLMRDCLGHRTGPLRTADLIGLDN
LADSLRVLHERTGDARCAPSELLLEKVRDGHLGRKSGRGFYAYGKELA

>putative oxidoreductase [GdmK]
ATGAGCGGACATGCGGAAGCGCACCGGCTCATGGTGCTGGGTGCGGGAACAATGGGACTG
GGGATCACCTCTCTCGCCGTGGGGCACGGAATCCCGGTGACGGTCGTCGAGGTGGACGAG
GCCAAGGCCGGCCGGACGCGGGCCGCGGTCACCGAACGGCTCCGCATGGCCCAGCTCATG
CGGGCCCTGCCCGCCGACCGGCCGCCCGGCGAGCTGACGGTCACCGCGTCCCCGGCCGAC
GGCCGGGAGGCCACCGCCGTGGTGGAGGCCGTCACCGAGGACACCCCCACCAAGGCCAAG
GTGCTCGAAGCGGTCGCCGGGCTCACCGGCGGGCGGGTGCCGCTGATCTCGAACACCTCC
TCCATCCCGATCGACGAACTGGCCGGTTTCATCGCCGATCCGGCACGGCTGGTCGGCACC
CACTTCATGAACCCGCCCTATCTGATCCCCACGGTGGAGGTGATCCGCGGCCCCCGTACC
GGGGAAGCGGCGATGGCCGCCGTGACGGATCTGCTGCGCGCGCTGGAGCGCAAGCCGGTC
GTCGTCGGCGACGGGCCGGGGTTCGTCACCAGCCGGGTGCTGCACCCGATGATCAACGAC
GCGATCCGTGTGGTGCAGGAGGGCACGGCGACGGCGGAGGCCGTGGACGTCCTCATGCGG
GACTGCCTGGGCCACCGCACCGGACCGCTGCGCACCGCGGACCTGATCGGCCTGGACAAC
CTCGCCGACTCGCTGCGCGTGCTGCATGAACGCACCGGCGACGCCCGGTGCGCACCGAGC
GAATTGCTGCTGGAGAAGGTCCGCGACGGCCATCTGGGCCGCAAGTCGGGCCGCGGGTTC
TACGCATACGGGAAGGAGCTGGCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain) PF00725 3HCDH IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding (Domain) PF02737 3HCDH_N IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain) SSF48179 6-phosphogluconate dehydrogenase C-terminal domain-like IPR013328 Dehydrogenase, multihelical (Domain) G3DSA:1.10.1040.10 no description IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 no description
SignalP	Bacteria, Gram-negative Eukaryota
TMHMM	No significant hit

Gelda_00070 Gelda_00090