Herb_00090 Herb_00090   Herb_00110 Herb_00110

Herb_00100 : CDS information

close this sectionLocation

Organism
StrainAM 3672
Entry nameHerbimycin A
Contig
Start / Stop / Direction19,043 / 18,360 / - [in whole cluster]
19,043 / 18,360 / - [in contig]
Locationcomplement(18360..19043) [in whole cluster]
complement(18360..19043) [in contig]
TypeCDS
Length684 bp (227 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative amino acid exporter
Product (GenBank)unknown
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF16p; ORF16; similar to predicted RhtB family transporters
Reference
ACC
PmId
[16085885] Insights into the biosynthesis of the benzoquinone ansamycins geldanamycin and herbimycin, obtained by gene sequencing and disruption. (Appl Environ Microbiol. , 2005)
comment
herbimycin生合成系gene clusterのクローニングを行い、同じbenzoquinone ansamycin系のgeldanamycin のgene clusterと比較し、一部の遺伝子欠損株を作成してその生合成における関与を調べた文献。

ORF16(684bp):RhtB family transporter
機能実験、詳細記載無し。
Related Reference
ACC
C6GYU4
NITE
Pimar_00010
PmId
[19505319] PimT, an amino acid exporter controls polyene production via secretion of the quorum sensing pimaricin-inducer PI-factor in Streptomyces natalensis. (Microb Cell Fact. , 2009)
comment
3rd(C6GYU4) 88%, 1e-102
Streptomyces natalensis_pimT
PimT protein

pimaricin gene clusterに隣接するRhtB family amino-acid exporter PimTについての論文。

pimTのgene replacement mutantは、L-homoserine, L-serine, and L-homoserine lactoneへの感受性増加。相補で回復。

pimT発現はhomoserineとquorum-sensing pimaricin-inducer PI-factor(autoinducer)によって誘導される。
pimT deletion mutantはpimaricin産生を65%、PI産生を50%まで減らし、pimT相補、外因性PI-factorの添加でpimaricin産生は回復する。
これはPimTがPI-factor exporterとして使用されることを示す。

以上から、pimaricin産生はamino acid exporter PimTによるquorum sensing pimaricin-inducer PI-factorの分泌を介して調節されている。

close this sectionSequence

selected fasta
>putative amino acid exporter [unknown]
MSPGRSDSGGGGHVVSTESVLTFAAMSLLVIVIPGPSVLFVVGRALAHGRRTALATVLGN
VIGCYALVVAVAWGLGALVESSVALFMGVKLAGAAYLVYLGVQAFRHRREMRVANMDAPA
DERRGDLRSILDGILVGVTNPKGIVFFAAVLPQFVDHSAGHVPVQMMVLGLVPVTIGLVT
DTLWGLGASAARSWFARSDRRLSMVGGAGGLAMIGLGVTVAATGRAD
selected fasta
>putative amino acid exporter [unknown]
ATGTCCCCTGGACGGAGCGATTCAGGAGGTGGCGGACACGTGGTGTCCACGGAGAGTGTT
CTCACGTTCGCGGCGATGTCGCTCTTGGTGATCGTGATCCCGGGGCCGAGTGTGCTGTTC
GTGGTCGGCAGGGCCCTCGCCCACGGCCGCCGCACGGCGCTCGCGACGGTCCTCGGCAAT
GTGATCGGCTGCTACGCCCTGGTGGTCGCCGTGGCGTGGGGCCTCGGCGCGCTGGTGGAG
AGCTCGGTGGCGCTGTTCATGGGCGTGAAGCTGGCAGGAGCGGCGTATCTCGTCTACCTG
GGTGTGCAGGCGTTCCGGCACCGCAGGGAGATGCGCGTGGCGAACATGGACGCTCCGGCC
GATGAGCGGCGTGGTGATCTGCGTTCGATCCTGGACGGCATTTTGGTGGGGGTCACCAAC
CCGAAGGGCATCGTCTTCTTCGCGGCGGTGCTGCCGCAGTTCGTCGACCACTCGGCGGGT
CATGTTCCCGTCCAGATGATGGTGCTGGGCCTGGTCCCGGTCACCATCGGCCTGGTCACG
GACACCTTGTGGGGCCTGGGCGCCTCGGCGGCCCGCTCCTGGTTCGCCCGCTCGGACCGC
CGCCTCTCGATGGTCGGCGGGGCGGGCGGCCTCGCGATGATCGGCCTGGGTGTGACGGTG
GCGGCGACCGGCCGCGCCGACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001123 Lysine-type exporter protein (LYSE/YGGA) (Family)
[29-221] 1.9e-28 PF01810
PF01810   LysE
SignalP
[1-47] 0.35 Signal
Bacteria, Gram-positive   
[1-1] Signal-anchor
Eukaryota   
TMHMM
[20-42] Transmembrane (o-i) [54-73] Transmembrane (i-o) [83-105] Transmembrane (o-i) [130-152] Transmembrane (i-o) [167-189] Transmembrane (o-i) [202-224] Transmembrane (i-o)
Transmembrane 6   
Herb_00090 Herb_00090   Herb_00110 Herb_00110
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