Grana_00020 Grana_00020   Grana_00040 Grana_00040

Grana_00030 : CDS information

close this sectionLocation

Organism
StrainTü22
Entry nameGranaticin
Contig
Start / Stop / Direction3,462 / 4,313 / + [in whole cluster]
3,462 / 4,313 / + [in contig]
Location3462..4313 [in whole cluster]
3462..4313 [in contig]
TypeCDS
Length852 bp (283 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative transcriptional regulator
Product (GenBank)pathway-specific transcriptional activator
Gene
Gene (GenBank)gra-orf9
EC number
Keyword
  • SARP family
Note
Note (GenBank)
Reference
ACC
PmId
[9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998)
[15358431] Widespread activation of antibiotic biosynthesis by S-adenosylmethionine in streptomycetes. (FEMS Microbiol Lett. , 2004)
comment
[PMID: 9831526](1998)
entire granaticin gene cluster(gra cluster)の報告。

ORF9: pathway-specific transcriptional activetor
actII-ORF4 homologue

--
[PMID:15358431](2004)
granaticinは生合成経路ではSAM-dependent methylationを必要としないが、低濃度のSAMで処理されたとき、産生が約2倍に増えた。
SAMのメチル化産物であるSAHでも同様の効果あり。
pathway-specific regulatorであるgra-orf9の転写は外因性SAM処理によって活性化される。
Related Reference
ACC
P25047
NITE
Adria_00210
PmId
[1729206] Regulation of secondary metabolism in Streptomyces spp. and overproduction of daunorubicin in Streptomyces peucetius. (J Bacteriol. , 1992)
[7868593] Functional characterization and transcriptional analysis of the dnrR1 locus, which controls daunorubicin biosynthesis in Streptomyces peucetius. (J Bacteriol. , 1995)
[8971703] Purification and characterization of the DNA-binding protein DnrI, a transcriptional factor of daunorubicin biosynthesis in Streptomyces peucetius. (Mol Microbiol. , 1996)
[16545946] Feedback regulation of doxorubicin biosynthesis in Streptomyces peucetius. (Res Microbiol. , 2006)
[20529134] Modulation of dnrN expression by intracellular levels of DnrO and daunorubicin in Streptomyces peucetius. (FEMS Microbiol Lett. , 2010)
[20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010)
comment
6th(P25047) 52%, 1e-64
Streptomyces peucetius_dnrI
Regulatory protein dnrI
[Adria_00210]transcriptional activator

--
[PMID: 1729206](1992)
ATCC 29050 strainでdnrI を不活化したら、RHO産生とdaunorubicin (DNR)産生ができなくなり、DNRへの抵抗性が著しく減った。

---
同じ著者らの続報。
[PMID: 7868593](1995)
dnrI::aphII mutantでは、DNR生合成におけるearly- and late-acting enzymesの両方をコードする転写物が存在しない。よってdnrI は、直接または間接的にDNR生合成遺伝子のすべてを調節している。

dnrI::aphII and dnrN::aphII strainsでresistance genes=drrAB and drrC転写物は消失。
よって、dnrI はDNR抵抗性の重大なregulatorであり、dnrNはdnrI を通して間接的にその影響を発現する、と示唆される。

--
[PMID: 8971703](1996) abstract
DNA-binding assays.
・dnrG-dpsABCD と dpsEF の間
・dnrC gene と dnrDKPSQ の間
の遺伝子間領域を含んだDNA断片へ特異的に結合する。

--
[PMID: 16545946](2006) abstract
dnrI promoter活性を増やすためにはDnrO and DnrN の両方が必要であった。

--
[PMID: 20529134]や[PMID: 20158521]で、dnrO, dnrN, dnrI, DNRが関与する細胞内DNR濃度維持システムが提唱されている。
dnrI promoterにはDnrNが結合し活性化される。

close this sectionSequence

selected fasta
>putative transcriptional regulator [pathway-specific transcriptional activator]
MDVGILGPLAVRLNGQSIVPSAGKPRQLLALLAIRAERVVTVPTLMEEIWGDAIPKSAAT
TLQTYILHLRRKVTAALRAGQPGAAADRAGTGAGAGDAKSVLSTCFGGYRLALRPEDSDL
REFQSLAARGSVALEAGDARAASAMLERALGLWRGTALVDVPVGRVLGVEVLGMEEQRTR
VLEQRIEADLLLGRHASLLAELRMLVARHPMNENLAAQLMTALYRSGGVWRALEVFQELR
RTLIEELGVEPSHRLQLLHRAVLSGELELDPRHAGLELMRTGG
selected fasta
>putative transcriptional regulator [pathway-specific transcriptional activator]
ATGGATGTTGGGATTCTCGGACCGCTCGCTGTCCGTCTGAACGGCCAGTCGATCGTGCCC
AGTGCGGGAAAGCCGCGCCAACTGCTCGCGCTGCTGGCCATCCGGGCGGAACGAGTGGTG
ACGGTGCCGACGCTGATGGAGGAGATCTGGGGTGACGCGATTCCCAAGAGCGCGGCGACC
ACACTGCAGACGTACATCCTCCATCTGCGCCGGAAGGTGACCGCGGCGCTGCGCGCCGGG
CAGCCGGGCGCCGCCGCTGATCGCGCCGGAACCGGGGCCGGGGCCGGGGACGCCAAGTCC
GTGCTCAGCACGTGCTTCGGCGGCTACCGCCTCGCCCTTCGCCCGGAGGACAGTGATCTG
CGCGAGTTCCAGTCCCTCGCGGCCCGTGGCTCGGTAGCGCTCGAGGCCGGTGACGCGCGC
GCCGCGTCCGCGATGCTGGAGCGGGCGCTCGGACTATGGCGCGGCACGGCGCTGGTGGAC
GTGCCGGTCGGACGCGTCCTGGGGGTGGAGGTCCTCGGCATGGAGGAGCAGCGCACCCGC
GTACTGGAGCAGCGCATAGAGGCGGACCTGCTGCTCGGGCGGCACGCCTCGCTCCTGGCC
GAGCTGCGGATGCTGGTCGCCCGGCACCCGATGAACGAGAACCTCGCCGCCCAGCTGATG
ACCGCGCTGTACCGGTCGGGCGGGGTCTGGCGGGCGCTGGAGGTCTTCCAGGAACTGCGC
AGGACGCTGATCGAGGAGCTGGGGGTGGAGCCCTCGCACCGGCTGCAGCTGCTGCACCGG
GCGGTGCTCTCCGGTGAGCTCGAGCTGGATCCCCGGCACGCCGGGCTCGAACTCATGCGG
ACCGGCGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001867 Signal transduction response regulator, C-terminal (Domain)
[15-111] 3.09999999999998e-22 PF00486
PF00486   Trans_reg_C
[15-111] 8.49999291746323e-11 SM00862
SM00862   Trans_reg_C
IPR005158 Bacterial transcriptional activator domain (Domain)
[118-263] 1e-47 SM01043
SM01043   BTAD
[119-263] 7.5e-47 PF03704
PF03704   BTAD
IPR011990 Tetratricopeptide-like helical (Domain)
[124-239] 0.00016 G3DSA:1.25.40.10
G3DSA:1.25.40.10   TPR-like_helical
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[23-112] 5.8e-10 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
[1-76] 6.49999377884395e-12 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP No significant hit
TMHMM No significant hit
Grana_00020 Grana_00020   Grana_00040 Grana_00040
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