Grana_00030 Grana_00030   Grana_00050 Grana_00050

Grana_00040 : CDS information

close this sectionLocation

Organism
StrainTü22
Entry nameGranaticin
Contig
Start / Stop / Direction5,026 / 4,418 / - [in whole cluster]
5,026 / 4,418 / - [in contig]
Locationcomplement(4418..5026) [in whole cluster]
complement(4418..5026) [in contig]
TypeCDS
Length609 bp (202 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative two-component system response regulator
Product (GenBank)putative two-component response regulator
Gene
Gene (GenBank)gra-orf10
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998)
comment
entire granaticin gene cluster(gra cluster)の報告。

ORF10: Response regulator of two-component system

配列解析のみ。
gra-ORF11 and gra-ORF10の産物は、細菌のtwo-component sensory kinase/response regulator pairs(S.subtilis_degS and degU)に似ている。
Related Reference
ACC
Q54821
NITE
Adria_00100
PmId
[7868594] Regulation of daunorubicin production in Streptomyces peucetius by the dnrR2 locus. (J Bacteriol. , 1995)
[8892835] The DnrN protein of Streptomyces peucetius, a pseudo-response regulator, is a DNA-binding protein involved in the regulation of daunorubicin biosynthesis. (J Bacteriol. , 1996)
[10846224] The dnrO gene encodes a DNA-binding protein that regulates daunorubicin production in Streptomyces peucetius by controlling expression of the dnrN pseudo response regulator gene. (Microbiology. , 2000)
[20122958] The activator/repressor protein DnrO of Streptomyces peucetius binds to DNA without changing its topology. (Int J Biol Macromol. , 2010)
[20529134] Modulation of dnrN expression by intracellular levels of DnrO and daunorubicin in Streptomyces peucetius. (FEMS Microbiol Lett. , 2010)
[20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010)
comment
122nd(Q54821) 46%, 3e-44
Streptomyces peucetius_dnrN
Regulatory protein
[Adria_00100]transcriptional activator

---
[PMID: 7868594](1995)
DnrNはresponse regulatorに配列は似ているが、putative phosphorylation siteの置換(D55E, D55N)をしても活性が完全にはなくならず、近くにHKが見当たらないことからpseudo-response regulatorとも呼ばれている。

dnrN::aphII mutationはdnrIで相補可能。
dnrI::aphII mutantはdnrNで相補できない。
よってdrnNがdnrIの転写を活性化するというregulatory cascadeの存在が示唆される。

---
[PMID: 8892835](1996)
DnrNをE.coliで過剰発現し、抽出液でgel mobility shift analysis.
DnrNは高い親和性で、dnrI promoter領域に特異的に結合する。
wild-type DnrNとputative phosphorylation site置換(D55N)mutantで結合能力に違いがなく、DnrNのリン酸化はDNA bindingに必要ない。
dnrN発現がDNR産生と同時に起こり、DnrNのレベルはDNRを産生しないmutant株で低いので、dnrNとdnrIの発現はDNRによってfeedback調節されると推測される。


---
[PMID: 10846224](2000)
dnrO::aphII mutant strainはdaunorubicin (DNR)非産生。
このmutant strainにdnrN or dnrI を導入すると生合成可能。
dnrI 発現はDnrNが必要なことがわかっているので、dnrN発現にDnrOが必要ということが示される。

DnrO → dnrN = DnrN → dnrI = DnrI →DNR生合成genes

DnrOをE.coliで過剰発現し、抽出液でmobility shift DNA-binding assays。
DnrOが特異的にoverlapping dnrN/dnrO(p1) promoter regionへ結合するので、DnrOはdnrNとdnrOの両方の発現を調節するかもしれない。

---
[PMID: 20122958][PMID: 20529134][PMID: 20158521](2010)
dnrO, dnrN, dnrI, DNRが関与する細胞内DNR濃度維持システムが提唱されている。
DnrOはDnrNと競合してfeedback的にDNRの細胞内濃度を調節している。
ACC
O34723
PmId
[15090506] The membrane fluidity sensor DesK of Bacillus subtilis controls the signal decay of its cognate response regulator. (J Bacteriol. , 2004)
comment
193rd(O34723) 43%, 2e-40
B.subtilis_desR
Transcriptional regulatory protein desR

DesKのC末がautokinaseで、DesRにリン酸パスを行なっていることを示している。
また、DesKのN末にある膜貫通領域がsenseに必須であるとも言っている。

close this sectionSequence

selected fasta
>putative two-component system response regulator [putative two-component response regulator]
MIRILLAEDMEMVRGALVALLGLEDDLEVVAQVERGDKILPEALKVRPDVAIIDIGLPGI
DGIKAAALLAEELPSCRTLILTSLSRPGVLRRALDAKVAGFLPKDASPQDLAEAVRRIAA
GHRAIDPQLALAAWDSAESPLTEREMEVLRLTAEGDEAPEIAARLHLSTGTVRNYLTSVV
TKLNARNRVDAVRIAHESGWLV
selected fasta
>putative two-component system response regulator [putative two-component response regulator]
GTGATACGCATATTGCTGGCTGAGGACATGGAAATGGTCCGGGGGGCGCTCGTGGCGCTG
CTGGGCCTGGAGGACGACCTGGAAGTGGTCGCGCAGGTCGAGCGCGGCGACAAGATCCTT
CCCGAAGCGCTGAAGGTCCGGCCCGACGTGGCGATCATCGACATCGGCCTTCCCGGCATC
GACGGCATCAAGGCGGCCGCGCTCCTCGCCGAGGAGCTCCCCAGCTGTCGCACCCTCATC
CTGACGAGTCTGAGCCGCCCCGGCGTCCTGCGCCGGGCCCTGGACGCCAAGGTGGCGGGC
TTCCTGCCGAAGGACGCCTCGCCCCAGGACCTCGCCGAGGCGGTACGGCGGATCGCGGCC
GGCCACCGTGCCATCGACCCGCAGCTGGCCCTCGCCGCCTGGGACAGCGCCGAGAGCCCG
CTCACCGAGCGGGAGATGGAAGTGCTCCGTCTCACGGCCGAGGGCGACGAGGCACCGGAG
ATCGCCGCCCGGCTGCACCTGTCCACCGGAACCGTCCGGAACTACCTGACCTCGGTCGTC
ACCAAGCTGAACGCCCGCAACCGGGTCGACGCGGTGCGCATCGCGCACGAGTCCGGCTGG
CTGGTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000792 Transcription regulator LuxR, C-terminal (Domain)
[141-155] 3.1999989904635e-11 PR00038 [155-171] 3.1999989904635e-11 PR00038 [171-183] 3.1999989904635e-11 PR00038
PR00038   HTHLUXR
[138-195] 1.20000117458134e-26 SM00421
SM00421   HTH_LUXR
[139-195] 1.3e-18 PF00196
PF00196   GerE
[155-182] PS00622
PS00622   HTH_LUXR_1
[134-199] PS50043
PS50043   HTH_LUXR_2
IPR001789 Signal transduction response regulator, receiver domain (Domain)
[2-115] 1.70000295590054e-18 SM00448
SM00448   REC
[4-116] 1.2e-16 PF00072
PF00072   Response_reg
[3-119] PS50110
PS50110   RESPONSE_REGULATORY
IPR011006 CheY-like superfamily (Domain)
[1-132] 3.80000697093586e-25 SSF52172
SSF52172   CheY_like
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[121-201] 1.5e-25 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
[119-201] 6.79998707392904e-21 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP
[1-15] 0.359 Signal
Eukaryota   
TMHMM No significant hit
Grana_00030 Grana_00030   Grana_00050 Grana_00050
Page top