Grana_00050 : CDS information

Location

Organism	Streptomyces violaceoruber
Strain	Tü22
Entry name	Granaticin
Contig	AJ011500
Start / Stop / Direction	6,345 / 5,125 / - [in whole cluster] 6,345 / 5,125 / - [in contig]
Location	complement(5125..6345) [in whole cluster] complement(5125..6345) [in contig]
Type	CDS
Length	1,221 bp (406 aa)

Annotation

Category	5.1 general function
Product	putative two-component system histidine kinase
Product (GenBank)	putative two-component sensory kinase
Gene
Gene (GenBank)	gra-orf11
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q9ZA46 PmId [9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998) comment entire granaticin gene cluster(gra cluster)の報告。 ORF11: Sensor kinase of two-component system 配列解析のみ。 gra-ORF11 and gra-ORF10の産物は、細菌のtwo-component sensory kinase/response regulator pairs(S.subtilis_degS and degU)に似ている。
Related Reference	ACC O34757 PmId [15090506] The membrane fluidity sensor DesK of Bacillus subtilis controls the signal decay of its cognate response regulator. (J Bacteriol. , 2004) comment Blast 201st, id32%, 2e-18, N末イマイチ(膜貫通ドメインあり) B.subtilis_desK Sensor histidine kinase desK DesKのC末がautokinaseで、DesRにリン酸パスを行なっていることを示している。また、DesKのN末にある膜貫通領域がsenseに必須であるとも言っている。
Family/Domain	FD IPR011712 comment [IPR011712] Signal transduction histidine kinase, subgroup 3, dimerisation and phosphoacceptor domain (Domain) function; GO:0000155 two-component sensor activity GO:0046983 protein dimerization activity

Sequence

>putative two-component system histidine kinase [putative two-component sensory kinase]
MNSREQQFDELDSSASYLSHEDSDLPAPRLARTVLLAAMAGYSLVTVVNILGAGLSPAET
CVGFTALLVVFGLQVAHSRPQARQSPYWRRVTTLTLQAVFCFVPLALFHMAWGAMGGFLA
GSVLLLLPGRAAWPLYGAIGASLFAYSAAAGHTLLDSVYTLEATLVTGLVVFGLSSLTGL
VTRLHAHRADLARLAVAGERLRFSRDLHDLLGYNLSAITLKTELVRRLVPDMPERARDET
DSILALSRQSLADVRKVATGYRDMSLREEAAATRSMLEAAGIEVEAAIDIGPLPSQIDTV
LATVLREGVTNALRHSRVHHCTIRCWEKDGVVTLSLGNDGVDPEDGGYEGVDRDARRADD
PGGTGLGNLTYRLTRVGGSLTTELSDNGWFRMTARAPVHPHVCTTR

>putative two-component system histidine kinase [putative two-component sensory kinase]
ATGAACTCACGTGAACAGCAGTTCGACGAGCTGGACAGCAGTGCCTCGTACCTCTCTCAC
GAGGACTCCGACCTGCCCGCCCCGAGGCTGGCCCGCACCGTCCTGCTGGCCGCGATGGCC
GGCTATTCCCTGGTCACGGTGGTGAACATCCTGGGCGCCGGACTGTCACCCGCCGAAACC
TGTGTCGGATTCACCGCACTGCTCGTGGTGTTCGGACTCCAGGTCGCCCACTCCCGCCCG
CAGGCCCGCCAGAGCCCTTACTGGCGCCGCGTGACCACCCTGACGTTGCAGGCCGTCTTC
TGTTTCGTACCGCTGGCGCTGTTTCACATGGCGTGGGGAGCGATGGGTGGCTTCTTGGCC
GGTTCCGTACTCCTGCTGCTGCCCGGCCGGGCGGCCTGGCCGCTCTACGGCGCGATCGGG
GCGTCGCTCTTCGCGTACTCGGCCGCGGCCGGCCACACCCTGCTCGACAGCGTCTACACC
TTGGAGGCCACGCTCGTCACCGGGCTCGTGGTGTTCGGACTGAGCAGCCTGACCGGTCTC
GTGACCCGGCTGCACGCGCACCGCGCCGACCTGGCCCGGCTCGCGGTCGCCGGCGAGCGG
CTCCGCTTCTCCCGCGACCTGCACGACCTGCTCGGCTACAACCTGTCCGCGATCACCCTC
AAGACCGAGCTGGTACGCCGCCTCGTGCCGGACATGCCGGAGCGGGCCAGGGACGAGACC
GACTCGATCCTCGCCCTCTCCCGCCAGTCGCTGGCCGACGTGCGCAAGGTCGCCACCGGC
TACCGCGACATGTCTCTGCGCGAGGAGGCCGCCGCCACCCGCTCGATGCTGGAGGCGGCG
GGCATAGAGGTGGAGGCGGCCATCGACATAGGCCCGCTGCCCTCCCAGATCGACACGGTG
CTCGCCACCGTGCTCCGCGAGGGCGTCACCAATGCCCTCCGGCACAGCCGCGTCCACCAC
TGCACCATCCGGTGCTGGGAGAAGGACGGCGTGGTCACGCTCAGCCTCGGCAACGACGGC
GTGGACCCGGAGGACGGCGGCTACGAGGGCGTGGACCGCGACGCGCGGAGGGCGGACGAC
CCGGGCGGCACCGGCCTCGGCAACCTCACCTACCGACTGACCCGCGTCGGCGGCAGCCTC
ACCACCGAGCTGAGCGACAACGGCTGGTTCCGGATGACCGCCCGGGCCCCGGTGCACCCG
CACGTCTGCACCACCCGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003594 ATPase-like, ATP-binding domain (Domain) PF02518 HATPase_c SSF55874 ATP_bd_ATPase G3DSA:3.30.565.10 ATP_bd_ATPase IPR011712 Signal transduction histidine kinase, subgroup 3, dimerisation and phosphoacceptor domain (Domain) PF07730 HisKA_3
SignalP	Eukaryota
TMHMM	Transmembrane 4

Grana_00040 Grana_00060