Grana_00120 : CDS information

Location

Organism	Streptomyces violaceoruber
Strain	Tü22
Entry name	Granaticin
Contig	AJ011500
Start / Stop / Direction	15,086 / 15,694 / + [in whole cluster] 15,086 / 15,694 / + [in contig]
Location	15086..15694 [in whole cluster] 15086..15694 [in contig]
Type	CDS
Length	609 bp (202 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	putative cyclase-dehydratase
Gene	gra-orf18
Gene (GenBank)	gra-0rf-18
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q9ZA41 PmId [9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998) comment entire granaticin gene cluster(gra cluster)の報告。 ORF18: cyclase-dehydratase? 配列解析のみ。 actVI-ORF3 homologue
Related Reference	ACC Q9ADD7 NITE Actino_00050 PmId [10805574] Chemical characterisation of disruptants of the Streptomyces coelicolor A3(2) actVI genes involved in actinorhodin biosynthesis. (J Antibiot (Tokyo). , 2000) [12421300] Primary and secondary metabolism, and post-translational protein modifications, as portrayed by proteomic analysis of Streptomyces coelicolor. (Mol Microbiol. , 2002) comment Blast 4th, id35%, 1e-20 Streptomyces coelicolor_SCO5074 Putative dehydratase [Actino_00050]hypothetical protein_actVI-ORF3 -- [PMID: 10805574](2000) ORF3破壊株でもactinorhodin産生あり。wildの55%。 -- [PMID:12421300](2002) 菌糸体抽出液のプロテオミクス解析。全長ActVI-ORF3は検出されない。 N末31 or 34aaが切断されたformで検出され、SignalP予測もこれに一致する。 whole cell extractよりexternal SDS extractで検出される。よって、ActVI-ORF3は細胞壁マトリックスに排出されると示唆される。

Sequence

>hypothetical protein [putative cyclase-dehydratase]
MPALRSRTRTAAIAVPLALAGLMATTSGAQAAPVKTYFDCRVGTVTGLAYSTYVDGVAPA
HVRPGATFDVTLDPAPITPNPAYNKEVKNVALQFKLPAGAKVLSYEVADRLAAGTPELQI
DGTKAVLRAAGPYVANAPFDLPKITIKLKAPTTRGTLETPFGGTSHDDPGFTWTFISPNW
TGEAQLACWPDQPIALTKTLVK

>hypothetical protein [putative cyclase-dehydratase]
ATGCCCGCACTGCGCAGCCGTACCCGTACCGCCGCCATAGCCGTCCCGCTGGCCCTGGCC
GGTCTGATGGCCACCACCTCGGGAGCCCAGGCCGCCCCCGTGAAGACCTACTTCGACTGC
CGCGTCGGCACCGTCACCGGTCTCGCGTACAGCACGTACGTCGACGGCGTGGCCCCGGCC
CACGTCCGGCCCGGAGCGACCTTCGACGTCACGCTCGACCCGGCCCCGATCACCCCGAAC
CCCGCCTACAACAAGGAGGTCAAGAACGTCGCGCTGCAGTTCAAGCTGCCCGCCGGCGCC
AAGGTCCTCTCCTACGAGGTCGCCGACCGGCTGGCCGCCGGCACCCCCGAGCTGCAGATC
GACGGCACCAAGGCCGTGCTGCGCGCCGCCGGGCCGTACGTCGCCAACGCCCCCTTCGAC
CTGCCGAAGATCACCATCAAGCTGAAGGCCCCCACCACCCGGGGCACCCTGGAGACGCCC
TTCGGCGGCACGTCGCACGACGACCCGGGCTTCACCTGGACCTTCATCTCCCCGAACTGG
ACCGGCGAGGCCCAGCTCGCCTGCTGGCCCGACCAGCCGATCGCGCTGACCAAGACGCTG
GTCAAGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro	No significant hit
SignalP	Bacteria, Gram-positive Bacteria, Gram-negative Eukaryota
TMHMM	Transmembrane 1

Grana_00110 Grana_00130