Grana_00100 Grana_00100   Grana_00120 Grana_00120

Grana_00110 : CDS information

close this sectionLocation

Organism
StrainTü22
Entry nameGranaticin
Contig
Start / Stop / Direction13,970 / 14,935 / + [in whole cluster]
13,970 / 14,935 / + [in contig]
Location13970..14935 [in whole cluster]
13970..14935 [in contig]
TypeCDS
Length966 bp (321 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductdTDP-glucose 4,6-dehydratase
Product (GenBank)putative dTDP-glucose-4,6-dehydratase
Genegra-orf17
graE
Gene (GenBank)gra-orf17
EC number4.2.1.46
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[7476861] Identification of Streptomyces violaceoruber Tu22 genes involved in the biosynthesis of granaticin. (Mol Gen Genet. , 1995)
[9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998)
comment
[PMID: 7476861](1995) abstract
ORF2(=gra-orf17)を含む7.3kbp領域の配列解析など。

ORF2によってコードされるGraEのAA配列はStrEに似ている。
E.coliでORF2を発現させると、dTDP-glucose→dTDP-4-keto-6-deoxy-D-glucoseへの変換を触媒することができた(controlの5倍)。よって、ORF2はdTDP-glucose dehydrataseをコードする。

--
[PMID: 9831526](1998)
entire granaticin gene cluster(gra cluster)のまとめ報告。

renameあり。
ORF2→ORF17
ORF3→ORF16

close this sectionSequence

selected fasta
>dTDP-glucose 4,6-dehydratase [putative dTDP-glucose-4,6-dehydratase]
MRLLVTGAAGFIGSHYVREILAGSYPESDDVHVTVVDRLTYAGRRDNLPEHHERLDFVHG
DICDRDLLDRVLPGHDAVVHFAAESHVDRSLTGPGEFVRTNVMGTQQLLDAALHAGVDRV
LHVSTDEVYGSLDSGTWTEDSPLLPNSPYAASKASTTWSAAPTTVRHGLDVRITRCSNNY
GPRQHPEKLIPNFVTRLLTGRQVPLYGDGRNVREWLHVDDHCRALQLVLTKGRAGEIYNI
GGGSGMSNREMTARLLDLLGADWDMVRHVEDRLGHDFRYAIDDSKIREELGYAPRWSIES
GLGAVVDWYRDHPDFWRAPAS
selected fasta
>dTDP-glucose 4,6-dehydratase [putative dTDP-glucose-4,6-dehydratase]
ATGAGGCTCCTGGTCACCGGCGCGGCCGGGTTCATCGGCTCGCACTACGTGCGCGAGATA
CTGGCGGGGAGCTACCCGGAGTCCGACGACGTCCACGTCACGGTCGTCGACCGGCTCACC
TACGCGGGGCGCCGCGACAACCTGCCCGAGCACCACGAACGGCTCGACTTCGTCCACGGC
GACATCTGCGACCGCGACCTCCTGGACCGGGTCCTCCCCGGCCACGACGCGGTCGTGCAC
TTCGCCGCCGAGTCCCACGTGGACCGCTCCCTCACCGGCCCCGGCGAGTTCGTCCGTACG
AACGTCATGGGCACCCAGCAGCTGCTCGACGCCGCACTCCACGCCGGTGTGGACCGAGTC
CTGCACGTCTCCACGGACGAGGTGTACGGCTCGTTGGACAGCGGCACCTGGACCGAGGAC
AGCCCCCTGCTGCCCAACTCCCCCTACGCGGCGTCCAAGGCCTCCACGACCTGGTCCGCC
GCGCCTACCACCGTACGCCACGGACTCGACGTCCGCATCACCCGCTGCTCCAACAACTAC
GGCCCGCGCCAGCACCCCGAGAAGCTCATCCCGAACTTCGTCACCCGGCTGCTGACCGGT
CGGCAGGTTCCGCTGTACGGCGACGGACGCAACGTGCGCGAGTGGCTGCACGTGGACGAC
CACTGCCGGGCGCTGCAACTGGTGCTCACCAAGGGCCGCGCGGGCGAGATCTACAACATC
GGCGGTGGCAGCGGTATGAGCAACCGCGAGATGACCGCCCGCCTGCTGGACCTCCTGGGG
GCGGACTGGGACATGGTGCGCCATGTCGAGGACCGCCTCGGGCACGACTTCCGCTATGCG
ATCGACGACTCCAAGATCCGCGAGGAGCTGGGCTACGCGCCCCGCTGGTCGATCGAGAGC
GGGCTGGGGGCGGTCGTGGACTGGTACCGCGACCACCCCGACTTCTGGCGCGCCCCGGCC
TCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
[4-241] 8.70000000000003e-69 PF01370
PF01370   Epimerase
IPR005888 dTDP-glucose 4,6-dehydratase (Family)
[2-317] 6.60000000000002e-118 TIGR01181
TIGR01181   DTDP_gluc_dehyt
IPR016040 NAD(P)-binding domain (Domain)
[1-178] 2.59999999999998e-62 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-22] 0.714 Signal
Eukaryota   
TMHMM No significant hit
Grana_00100 Grana_00100   Grana_00120 Grana_00120
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