Grana_00100 : CDS information

Location

Organism	Streptomyces violaceoruber
Strain	Tü22
Entry name	Granaticin
Contig	AJ011500
Start / Stop / Direction	12,912 / 13,973 / + [in whole cluster] 12,912 / 13,973 / + [in contig]
Location	12912..13973 [in whole cluster] 12912..13973 [in contig]
Type	CDS
Length	1,062 bp (353 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative glucose-1-phosphate thymidylyltransferase putative dTDP-glucose synthase
Product (GenBank)	putative dTDP-1-glucose synthase
Gene	gra-orf16 graD
Gene (GenBank)	gra-orf16
EC number	2.7.7.24
Keyword
Note
Note (GenBank)
Reference	ACC Q9ZA42 PmId [7476861] Identification of Streptomyces violaceoruber Tu22 genes involved in the biosynthesis of granaticin. (Mol Gen Genet. , 1995) [9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998) comment [PMID: 7476861](1995) abstract ORF3(=gra-orf16)を含む7.3kbp領域の配列解析。 ORF3によってコードされるAA配列(GraD)はStreptomyces griseusのdTDP-glucose synthase_StrDに似ている。 -- [PMID: 9831526](1998) entire granaticin gene cluster(gra cluster)の報告。 renameあり。 ORF2→ORF17 ORF3→ORF16 ORF16: dTDP-1-glucose synthase [sugar] gra-orf16については配列解析のみ。 dTDP-1-glucose synthase(EC 2.7.7.24)として機能すると提唱されている。
Related Reference	ACC Q2HR55 NITE Sch475_00270 PmId [18051285] Cloning and expression of glucose-1-phosphate thymidylyltransferase gene (schS6) from Streptomyces sp. SCC-2136. (J Microbiol Biotechnol. , 2007) comment 5th(Q2HR55) 58%, 1e-116 Streptomyces sp. SCC 2136_schS6 Putative dNDP-glucose synthetase schS6をE.coliで発現、精製、酵素活性測定。 glucose-1-phosphate thymidylyltransferase活性を示す。 dTTP + alpha-D-glucose-1-phosphate → dTDP-D-glucose + pyrophosphate
Related Reference	ACC Q194R4 NITE Mith_00060 PmId [9150235] Cloning and insertional inactivation of Streptomyces argillaceus genes involved in the earliest steps of biosynthesis of the sugar moieties of the antitumor polyketide mithramycin. (J Bacteriol. , 1997) comment 20th(Q194R4) 55%, 1e-102 Streptomyces argillaceus_mtmD DTDP-glucose synthase E. coli発現タンパクで以下の2酵素の活性見ている。 mtmD: glucose-1-phosphate:dTTP thymidylyl transferase mtmE: dTDP-D-glucose 4,6-dehydratase mtmD mutant -- accumulates premithramycinone

Sequence

>putative glucose-1-phosphate thymidylyltransferase [putative dTDP-1-glucose synthase]
MKALILSGGMGTRLRPFTYSMPKQLVPVANKPILVHCLENVRAIGVEEVAVVVGDRADEV
RAVVGDGSAFGLDVTYLQQEAPLGLAHCVSIAEEFLGDEDFVMYLGDNILAEGIAESARA
FRDERSAARLLLTKVADPRAYGVAETDATGRVHALVEKPERPRSDLAVIGVYFFTAAVHD
AVRAIEPSARGELEITDAIQYLVERGDRVVADEYTGYWKDTGSPDDLLDCNRVLLGRLRP
GVHGEVDAASTVEGTVVVEAGRSWSDSRLVGPLVVGAGSVVRGSELGPYTALGRDCVLED
AGIRDSIVLDGVSIQGVRGLSGSLIGRSAAVRTGEAAGRRLIIGDHTQAEVAA

>putative glucose-1-phosphate thymidylyltransferase [putative dTDP-1-glucose synthase]
ATGAAGGCGCTGATTCTGTCCGGAGGGATGGGGACCAGGTTGCGTCCCTTCACCTATTCC
ATGCCCAAACAACTGGTGCCCGTCGCCAACAAGCCGATCCTCGTGCACTGCCTGGAGAAC
GTCCGTGCCATCGGTGTCGAGGAGGTCGCCGTCGTCGTCGGCGACCGCGCCGACGAGGTC
CGGGCGGTGGTCGGTGACGGCTCCGCGTTCGGCCTCGACGTCACCTACCTCCAGCAGGAG
GCGCCGCTCGGCCTCGCTCACTGCGTCTCCATCGCCGAGGAGTTCCTCGGTGACGAGGAC
TTCGTGATGTACCTGGGCGACAACATCCTCGCTGAGGGCATCGCCGAGTCCGCCCGCGCG
TTCCGCGACGAACGTTCCGCCGCCCGGCTGCTGCTCACCAAGGTGGCCGACCCGCGTGCC
TACGGCGTCGCCGAGACGGATGCCACCGGCAGGGTCCACGCGCTGGTCGAGAAGCCCGAG
CGGCCGCGCAGCGACCTCGCCGTGATCGGCGTGTACTTCTTCACCGCCGCCGTCCACGAC
GCCGTCCGGGCGATCGAGCCGAGCGCCCGCGGCGAACTGGAGATCACCGACGCGATCCAG
TACCTCGTGGAGCGCGGCGACCGGGTCGTCGCCGACGAGTACACCGGCTACTGGAAGGAC
ACCGGCAGCCCCGACGACCTGCTGGACTGCAATCGGGTACTCCTCGGCCGGCTCCGCCCC
GGCGTACACGGCGAGGTCGACGCGGCCAGTACCGTCGAGGGGACCGTGGTCGTCGAGGCG
GGACGGTCGTGGAGCGACTCGCGCCTGGTCGGACCGCTCGTCGTCGGCGCCGGCAGCGTC
GTGCGCGGCAGCGAGCTCGGCCCGTACACCGCTCTCGGCCGCGACTGCGTGCTGGAGGAC
GCGGGGATCCGCGACTCCATCGTGCTCGACGGGGTGTCGATCCAGGGCGTGCGCGGGCTC
TCCGGCTCGCTCATCGGCCGCTCGGCCGCCGTGCGCACCGGCGAGGCGGCCGGACGGCGG
CTCATCATCGGGGACCATACGCAGGCGGAGGTGGCGGCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005835 Nucleotidyl transferase (Domain) PF00483 NTP_transferase IPR005908 Glucose-1-phosphate thymidylyltransferase, long form (Family) TIGR01208 RmlA_long
SignalP	Eukaryota
TMHMM	No significant hit

Grana_00090 Grana_00110