Grana_00080 Grana_00080   Grana_00100 Grana_00100

Grana_00090 : CDS information

close this sectionLocation

Organism
StrainTü22
Entry nameGranaticin
Contig
Start / Stop / Direction10,869 / 12,542 / + [in whole cluster]
10,869 / 12,542 / + [in contig]
Location10869..12542 [in whole cluster]
10869..12542 [in contig]
TypeCDS
Length1,674 bp (557 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative drug resistance transporter
Product (GenBank)putative transmembrane protein
Gene
Gene (GenBank)gra-orf15
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[7476861] Identification of Streptomyces violaceoruber Tu22 genes involved in the biosynthesis of granaticin. (Mol Gen Genet. , 1995)
[9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998)
comment
[PMID: 7476861](1995) abstract
ORF4(=gra-ORF15)を含む7.3kbp領域の配列解析。

tetracycline and methylenomycinに抵抗性を与えるタンパクと類似性あり。
よってactive efflux mechanismにより細胞外にgranaticinを移動するのに関連すると示唆される。

--
[PMID: 9831526](1998)
entire granaticin gene cluster(gra cluster)の報告。

ORF15: Transmembrane protein(Export)
actII-ORF2 homologue
Related Reference
ACC
Q53769
PmId
[8534971] A self-defense gene homologous to tetracycline effluxing gene essential for antibiotic production in Streptomyces aureofaciens. (Biosci Biotechnol Biochem. , 1995)
comment
20th(Q53769) 42%, 4e-86
Streptomyces aureofaciens_tcr3(tcrC)
Tcr3 protein

tcrC gene-inactivated strainsでtetracyclineへの抵抗性と抗生物質生産性は90%低下する。

close this sectionSequence

selected fasta
>putative drug resistance transporter [putative transmembrane protein]
MTDTANRRSATPDSGADAVDKRRIRLIMTGLLLLGLFMAALDQTIISAALRTIADDLNGL
SEQAWANTSYMITSVIMTALYGKLSDIYGRRPVYCTAVGVFVLGSVLCGLAQSMTTLAVF
RGQGIGAGGLMSLAFAILTDLVPLAERSRYQAWFGAVFGVSAVVGPVAGGFFAGLDSFLG
ASGWRWAFFINVPIGVAGALIIAALVRVQPGRAQHKFDIAGLLALIACLLPLLFAVEQGP
AWGWTDPGTLVLFGLGAVGLVLFSLAQKRAADAALLPTPMFRNPLFSVYNGVNVLVGAAV
FGALSVLPLYLQMVKGLSPTQAGLMMLPQTLGIVVAGRIARPYVTRTGRYKAVLLSGLVL
MVVATFWFGVLTADTSLWQTGVAAGVMGFGIGLCWQVMLIAIQTGVAPQYMGAGMGSFTF
FRQIGGTAGIAVFLSMFFGAVGEKVAAAYRGAASDPAYTAAVNDPAVTGQAANKVLLGPG
RRVGLDNSSFLDHADPRLAKPFLEGLAEAMQTVFVVSGVMLLIALVLAAVPKEKPPRAGR
RFAGRRKETGKQPAAKK
selected fasta
>putative drug resistance transporter [putative transmembrane protein]
ATGACAGACACAGCGAACCGCCGGTCCGCCACGCCCGATTCCGGTGCCGACGCGGTGGAC
AAGCGCAGAATCCGGCTGATCATGACGGGGCTGCTGCTGCTCGGCCTGTTCATGGCCGCC
CTCGACCAGACGATCATCTCGGCGGCGCTGCGTACCATCGCCGACGACCTCAACGGCCTC
TCCGAGCAGGCCTGGGCGAACACCTCGTACATGATCACCTCGGTGATCATGACGGCGCTG
TACGGAAAGCTCTCCGACATCTACGGACGCCGCCCGGTCTACTGCACGGCCGTCGGCGTC
TTCGTCCTCGGCTCCGTACTGTGCGGCCTCGCCCAGTCCATGACCACGCTCGCGGTCTTC
CGCGGCCAGGGCATCGGCGCGGGCGGCCTGATGAGCCTGGCCTTCGCGATCCTCACCGAC
CTGGTGCCGCTCGCCGAACGCAGCCGCTACCAGGCATGGTTCGGCGCGGTCTTCGGTGTC
TCGGCGGTCGTCGGCCCCGTCGCGGGCGGCTTCTTCGCCGGACTCGACAGCTTCCTCGGC
GCGTCCGGCTGGCGCTGGGCCTTCTTCATCAACGTGCCGATCGGTGTGGCGGGCGCGCTG
ATCATCGCCGCGCTGGTCCGCGTCCAGCCGGGACGCGCACAGCACAAGTTCGACATCGCG
GGGCTGCTCGCCCTGATCGCCTGCCTGCTGCCGCTGCTCTTCGCGGTCGAGCAGGGCCCC
GCGTGGGGCTGGACGGACCCCGGGACGCTCGTCCTGTTCGGCCTCGGAGCGGTCGGTCTG
GTGCTCTTCTCGCTGGCGCAGAAGCGGGCCGCCGACGCCGCGCTGCTGCCGACCCCGATG
TTCCGCAACCCGCTCTTCTCCGTCTACAACGGCGTGAACGTCCTGGTCGGAGCGGCGGTC
TTCGGCGCCCTGTCCGTCCTGCCGCTCTACCTCCAGATGGTCAAGGGCCTCTCGCCCACC
CAGGCGGGGCTGATGATGCTGCCGCAGACGCTGGGCATCGTCGTCGCCGGCCGGATCGCC
CGTCCGTACGTCACCAGGACGGGCCGCTACAAGGCGGTGCTGCTCAGCGGCCTGGTGCTG
ATGGTCGTCGCGACCTTCTGGTTCGGCGTGCTCACCGCGGACACCTCGCTGTGGCAGACC
GGTGTCGCCGCCGGGGTGATGGGCTTCGGCATCGGCCTGTGCTGGCAGGTCATGCTGATC
GCGATCCAGACGGGTGTCGCGCCGCAGTACATGGGGGCGGGCATGGGTTCGTTCACCTTC
TTCCGTCAGATCGGCGGTACGGCCGGCATCGCCGTCTTCCTGTCGATGTTCTTCGGCGCG
GTCGGCGAGAAGGTGGCCGCCGCCTACCGGGGCGCGGCCTCCGACCCGGCGTACACGGCG
GCCGTCAACGACCCGGCCGTGACCGGACAGGCCGCGAACAAGGTGCTGCTGGGCCCGGGA
CGGCGGGTGGGGCTGGACAACAGCTCCTTCCTCGATCACGCCGACCCGCGGCTGGCGAAG
CCCTTCCTGGAGGGCCTGGCCGAGGCGATGCAGACGGTCTTCGTGGTCAGCGGGGTCATG
CTGCTGATCGCCCTGGTGCTGGCGGCCGTGCCGAAGGAGAAGCCGCCGCGAGCCGGCCGC
CGGTTCGCCGGGCGCCGCAAGGAGACGGGGAAGCAGCCCGCGGCCAAGAAGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR011701 Major facilitator superfamily (Family)
[32-429] 2.19999999999997e-44 PF07690
PF07690   MFS_1
IPR016196 Major facilitator superfamily domain, general substrate transporter (Domain)
[1-552] 3.49999466863949e-71 SSF103473
SSF103473   MFS_gen_substrate_transporter
IPR020846 Major facilitator superfamily domain (Domain)
[28-536] PS50850
PS50850   MFS
SignalP
[1-1] Signal-anchor
Eukaryota   
TMHMM
[31-53] Transmembrane (i-o) [63-85] Transmembrane (o-i) [97-119] Transmembrane (i-o) [123-145] Transmembrane (o-i) [152-174] Transmembrane (i-o) [184-206] Transmembrane (o-i) [219-238] Transmembrane (i-o) [248-265] Transmembrane (o-i) [285-307] Transmembrane (i-o) [317-339] Transmembrane (o-i) [352-371] Transmembrane (i-o) [386-408] Transmembrane (o-i) [420-442] Transmembrane (i-o) [509-531] Transmembrane (o-i)
Transmembrane 14   
Grana_00080 Grana_00080   Grana_00100 Grana_00100
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