Grana_00070 Grana_00070   Grana_00090 Grana_00090

Grana_00080 : CDS information

close this sectionLocation

Organism
StrainTü22
Entry nameGranaticin
Contig
Start / Stop / Direction10,515 / 9,367 / - [in whole cluster]
10,515 / 9,367 / - [in contig]
Locationcomplement(9367..10515) [in whole cluster]
complement(9367..10515) [in contig]
TypeCDS
Length1,149 bp (382 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative C-glycosyltransferase
Product (GenBank)putative glycosyl transferase
Gene
Gene (GenBank)gra-orf14
EC number
Keyword
  • D-olivose
Note
Note (GenBank)
Reference
ACC
PmId
[7476861] Identification of Streptomyces violaceoruber Tu22 genes involved in the biosynthesis of granaticin. (Mol Gen Genet. , 1995)
[9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998)
comment
[PMID: 9831526](1998)
entire granaticin gene cluster(gra cluster)の報告。

PKS genesから約10kbpのところにあるfragmentから5ORFs(ORF1-5=gra-ORF18-14?)をクローニングしている[PMID: 7476861]での配列(Q56176)を修正。

gra-ORF14には、granaticin → granaticin Bへ変換するためのgranaticinへのrhodinose付着という通常のglycoside結合を割り当てている。

granaticin形成に至るC-glycosilationについては、1979年の論文を参照している。
Related Reference
ACC
Q9ZGC0
NITE
Lando_00200
PmId
[15977274] LanGT2 Catalyzes the First Glycosylation Step during landomycin A biosynthesis. (Chembiochem. , 2005)
[21513888] Rational design of an aryl-C-glycoside catalyst from a natural product O-glycosyltransferase. (Chem Biol. , 2011)
comment
Blast 5th, id45%, 8e-82
Streptomyces cyanogenus_lanGT2
Glycosyl transferase homolog
[Lando_00200]D-olivose glycosyltransferase

---
[PMID: 15977274](2005) abstract
lanGT2不活化mutantの産物は糖側鎖を欠損。よってLanGT2がhexasaccharide生合成においてpriming glycosyl transfer(polyketide骨格のO-8へのD-olivoseの付着)を触媒することを示す。

lanGT2 mutantでurdGT2を発現すると新規C-glycosylated angucyclineが産生されるのを確認している。
よってLanGT2とUrdGT2は似ているが異なる機能を持つ。

---
[PMID: 21513888](2011)
O-glycosyltransferase LanGT2を操作して、UrdGT2のようなC-Glycosylation活性を持つようにしている。
LanGT2, UrdGT2の反応メカニズムを提唱。
ACC
Q9RPA7
NITE
Urd_00200
PmId
[10658661] Two new tailoring enzymes, a glycosyltransferase and an oxygenase, involved in biosynthesis of the angucycline antibiotic urdamycin A in Streptomyces fradiae Tu2717. (Microbiology. , 2000)
[17640665] Structure and action of the C-C bond-forming glycosyltransferase UrdGT2 involved in the biosynthesis of the antibiotic urdamycin. (J Mol Biol. , 2007)
[12696864] The C-Glycosyltransferase UrdGT2 is unselective toward d- and l-configured nucleotide-bound rhodinoses. (J Am Chem Soc. , 2003)
comment
Blast 9th, id43%, 1e-74
Streptomyces fradiae_urdGT2
Glycosyl transferase
[Urd_00200]D-olivose C-glycosyltransferase

[PMID: 10658661](2000)
urdGT2が削除されたmutantは、urdamycin Aの代わりに、C-C connected deoxysugar moietyを欠いたtetracyclic angucyclinonesであるurdamycin I, urdamycin J and urdamycin Kを産生した。したがって、UrdGT2はNDP-D-olivoseのC-glycosyltransferを触媒する。

close this sectionSequence

selected fasta
>putative C-glycosyltransferase [putative glycosyl transferase]
MRFLFVSGGSAGAVFPITPLALAARNAGHEVIVGATENVMPLVAATGLPGAPITSRTMFD
FMQRDRHGNPLEIPKDPHERNLFNGRGMARLALGSMEGLVPLVERWQPDVLVAGALSYAA
PLVAHRFGLPWVRHALNMGEPSIIDLSAAAELAPELEEMGLSAIPEPDMYVEICPPGARR
PDAGPAQFMRYVPFNTQRALEPWMYAKGDRPRVLVSAGSRVTADYEADALSALVEKVAGL
DVELLIAAPQEIADALGDLPDNVRAGCVPLDVVLRTCDLLVHRAGGNTMLHAIVCGVPQL
VIPAMPKQVGMSARLAEYGAAIMLTAGQDDSPENVAKACRELLEDPAYKARTDELSREIA
GLPAPHDVTVAITEMVRARARG
selected fasta
>putative C-glycosyltransferase [putative glycosyl transferase]
ATGAGATTCCTTTTTGTGTCCGGAGGAAGCGCGGGGGCGGTCTTCCCCATCACCCCGCTG
GCGTTGGCGGCGCGCAACGCCGGCCACGAGGTGATCGTCGGAGCCACGGAGAACGTGATG
CCGCTGGTCGCCGCGACCGGACTGCCCGGCGCCCCGATCACCTCGCGCACCATGTTCGAC
TTCATGCAGCGCGACCGGCACGGCAATCCGCTGGAGATCCCCAAGGACCCTCACGAGCGG
AACCTGTTCAACGGCCGCGGCATGGCCCGCCTCGCCCTCGGCAGCATGGAGGGACTCGTT
CCGCTGGTCGAGCGCTGGCAGCCGGACGTCCTGGTCGCCGGAGCGCTCTCGTACGCCGCG
CCCCTGGTGGCCCACCGCTTCGGCCTGCCCTGGGTGCGCCACGCGCTCAACATGGGCGAG
CCGTCCATCATCGATCTCTCCGCCGCCGCCGAACTGGCGCCGGAGCTCGAGGAGATGGGC
CTGTCCGCGATACCCGAACCGGACATGTACGTGGAGATCTGCCCGCCCGGCGCCCGCCGC
CCGGACGCGGGGCCCGCACAGTTCATGCGATACGTCCCCTTCAACACGCAGCGGGCCCTG
GAGCCCTGGATGTACGCCAAGGGCGACCGGCCGCGCGTGCTGGTCTCGGCGGGCAGCCGG
GTCACCGCCGACTATGAGGCCGACGCCCTGTCCGCCCTGGTGGAGAAGGTCGCCGGACTC
GACGTGGAACTCCTCATCGCGGCACCGCAGGAGATCGCCGACGCCCTCGGCGATCTGCCG
GACAACGTGCGCGCCGGCTGCGTGCCGCTCGACGTCGTCCTGCGCACCTGTGACCTGCTG
GTGCACCGGGCCGGCGGCAACACGATGCTGCACGCGATCGTGTGCGGCGTCCCGCAGCTG
GTGATCCCCGCCATGCCGAAGCAGGTGGGGATGTCCGCCCGGCTCGCGGAGTACGGCGCC
GCCATCATGCTGACCGCCGGGCAGGACGACTCTCCCGAGAACGTGGCCAAGGCCTGCCGG
GAGTTGCTGGAGGACCCGGCGTACAAGGCCAGGACCGACGAGTTGAGCCGGGAGATCGCG
GGTCTGCCCGCGCCCCACGACGTGACGGTGGCGATCACCGAAATGGTGCGCGCCCGGGCC
CGGGGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002213 UDP-glucuronosyl/UDP-glucosyltransferase (Family)
[281-357] 1.2e-05 PF00201
PF00201   UDPGT
IPR004276 Glycosyl transferase, family 28 (Domain)
[3-141] 2.7e-22 PF03033
PF03033   Glyco_transf_28
IPR010610 Domain of unknown function DUF1205 (Domain)
[171-264] 3.3e-26 PF06722
PF06722   DUF1205
SignalP
[1-23] 0.934 Signal
Eukaryota   
[1-23] 0.992 Signal
Bacteria, Gram-negative   
[1-23] 0.968 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Grana_00070 Grana_00070   Grana_00090 Grana_00090
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