Grana_00180 : CDS information

Location

Organism	Streptomyces violaceoruber
Strain	Tü22
Entry name	Granaticin
Contig	AJ011500
Start / Stop / Direction	22,245 / 21,004 / - [in whole cluster] 22,245 / 21,004 / - [in contig]
Location	complement(21004..22245) [in whole cluster] complement(21004..22245) [in contig]
Type	CDS
Length	1,242 bp (413 aa)

Annotation

Category	5.3 uncharacterized protein (biosynthesis involved)
Product	hypothetical protein
Product (GenBank)
Gene
Gene (GenBank)	gra-orf24
EC number
Keyword	L-rhodinose
Note
Note (GenBank)
Reference	ACC Q9ZA35 PmId [9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998) [11269719] Identification of four genes from the granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22 involved in the biosynthesis of L-rhodinose. (J Antibiot (Tokyo). , 2001) comment [PMID: 9831526](1998) entire granaticin gene cluster(gra cluster)の報告。 ORF24: Unknown ORF25と翻訳共役。 -- [PMID: 11269719](2001) ORF22-25の各不活化mutantsは、dihydrogranaticin B産生ができなくなり、granaticinを蓄積する。 dihydrogranaticin Bはgranaticin Bの前駆体。 granaticin BはgranaticinにL-rhodinoseが追加された構造。よって、ORF22-25はどれもL-rhodinose形成・付着に関連。 pathwayでの割り当ては配列相同性から。でも、ORF24はDBで意味ある類似性が見られないので機能不明。可能性として挙げられているのが、 ORF23によって触媒される3-deoxygenation反応の完成に必要なreductase機能。または全体的な生合成経路の(dihydro)granaticin B分岐を特異的にコントロールするregulatory機能。

Sequence

>hypothetical protein
MKLLFVAPVVGTHGEVTTGLGLARLLAPAGITAHFVVDTHNAPQLEAAGVPGTLIDASMG
VGVRKVVEDVVRAERPDVVVLSDYLSYWLMMHRVYRTDPWFVERLGVPVIPLDLYEWENT
DFRIDHFGLDLEVDRRILDMPVQLRPAPWARPDPGPGSRALTYPFMRPLPRPTAEARRTV
RSSLGLRDGERLLSLSVSGWQRYAQTVEDPTTRGLAHRVPERVAAALRRLPPTTRFLVVG
PDLDGLEGLPAERTHRVPACPPDRYQELLDASDAVLSLHVPASALVRSVFSDLPAVYIGH
SGPPADGPVGPGEPDGPGRPDRPDGPDALAPYSMWPLRWNSVIRPLLTDNPLSETFRRTE
LLDPEGTVAALDAVLHDPAERTRLAEGRARYLAALDALPAVPEIFTEAVRRLG

>hypothetical protein
ATGAAGCTGCTGTTCGTCGCACCCGTCGTCGGCACCCACGGCGAGGTCACCACCGGGCTT
GGCCTGGCCCGGCTGCTCGCCCCCGCCGGGATCACCGCCCACTTCGTCGTGGACACCCAC
AACGCCCCGCAGCTCGAGGCCGCGGGCGTACCCGGCACCCTGATCGACGCGTCGATGGGA
GTCGGGGTCCGCAAGGTCGTCGAGGACGTCGTACGGGCCGAGCGGCCCGATGTGGTCGTG
CTCAGCGACTACCTCTCGTACTGGTTGATGATGCACCGCGTCTACCGAACCGACCCCTGG
TTCGTGGAACGGCTCGGCGTCCCGGTGATCCCGCTCGACCTGTACGAGTGGGAGAACACC
GACTTCCGCATCGACCACTTCGGGCTCGACCTGGAGGTGGACCGGCGGATCCTCGACATG
CCCGTCCAGCTGCGGCCCGCGCCGTGGGCGCGTCCCGACCCGGGCCCCGGCAGCCGAGCC
CTGACCTACCCCTTCATGCGGCCGCTGCCCCGCCCCACCGCCGAGGCGCGCCGCACCGTG
CGGAGCTCCCTCGGGCTGCGGGACGGGGAACGCCTCCTCTCGCTGTCCGTCTCGGGCTGG
CAGCGGTACGCCCAGACCGTCGAGGACCCGACGACCCGCGGGCTCGCCCACCGGGTGCCG
GAGCGGGTCGCGGCGGCGCTGCGCAGGCTTCCCCCCACCACCCGGTTCCTCGTCGTCGGG
CCTGATCTGGACGGTCTGGAAGGGCTGCCGGCCGAGCGCACCCACCGTGTGCCGGCCTGC
CCGCCCGACCGCTACCAGGAGCTCCTCGACGCCTCGGACGCCGTGCTGTCGCTGCACGTG
CCCGCCTCGGCGCTGGTCCGCTCGGTCTTCTCGGACCTGCCCGCGGTGTACATCGGCCAC
AGCGGCCCGCCCGCGGACGGACCGGTTGGCCCGGGCGAGCCGGACGGGCCGGGCAGGCCA
GACAGGCCGGACGGGCCGGACGCACTGGCCCCGTACAGCATGTGGCCGCTGCGCTGGAAC
TCCGTCATCCGGCCACTGCTCACCGACAACCCCCTGTCCGAGACCTTCCGGCGGACCGAA
CTCCTCGACCCCGAAGGGACGGTGGCGGCACTCGACGCCGTGCTCCACGATCCGGCCGAG
CGCACCCGGCTGGCCGAGGGACGCGCCCGCTATCTGGCGGCGCTCGACGCGCTGCCCGCC
GTGCCCGAGATCTTCACGGAGGCGGTCCGCCGGCTGGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro	No significant hit
SignalP	Eukaryota
TMHMM	No significant hit

Grana_00170 Grana_00190