Grana_00190 : CDS information

Location

Organism	Streptomyces violaceoruber
Strain	Tü22
Entry name	Granaticin
Contig	AJ011500
Start / Stop / Direction	22,829 / 22,242 / - [in whole cluster] 22,829 / 22,242 / - [in contig]
Location	complement(22242..22829) [in whole cluster] complement(22242..22829) [in contig]
Type	CDS
Length	588 bp (195 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose epimerase
Product (GenBank)	putative dTDP-4-keto-6-deoxyglucose-2,3-dehydratase
Gene
Gene (GenBank)	gra-orf25
EC number	5.1.3.-
Keyword	L-rhodinose
Note
Note (GenBank)
Reference	ACC Q9ZA34 PmId [9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998) [11269719] Identification of four genes from the granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22 involved in the biosynthesis of L-rhodinose. (J Antibiot (Tokyo). , 2001) comment UniProtでORF25とORF27のproduct nameが逆に登録されている模様。 --- [PMID: 9831526](1998) entire granaticin gene cluster(gra cluster)の報告。 ORF25: dTDP-4-keto-6-deoxyglucose-3,5-epimeraseに似ている。 dTDP-4-keto-2,3,6-trideoxyglucoseのC-5 methyl基の異性化が提唱されている。 -- [PMID: 11269719](2001) ORF22-25の各不活化mutantsは、dihydrogranaticin B産生ができなくなり、granaticinを蓄積する。 dihydrogranaticin Bはgranaticin Bの前駆体。 granaticin BはgranaticinにL-rhodinoseが追加された構造。よって、ORF22-25はどれもL-rhodinose形成・付着に関連。 pathwayでの割り当ては配列相同性から。
Related Reference	ACC Q9S0P2 NITE Aver_00150 PmId [11451669] Insights about the biosynthesis of the avermectin deoxysugar L-oleandrose through heterologous expression of Streptomyces avermitilis deoxysugar genes in Streptomyces lividans. (Chem Biol. , 2001) comment 56th(Q9S0P2) 49%, 8e-39 Streptomyces avermitilis_aveBV(avrF) dTDP-4-keto-6-deoxyhexose 3,5-epimerase [Aver_00150]dTDP-4-keto-6-deoxyhexose 5-epimerase --- [PMID: 11451669](2001) Table1. avrF/aveBV: dTDP-4-keto-6-deoxyglucose 3-epimerase　 (本文を読むと5-epimeraseの間違いと思われる。) S.lividansでS.avermitilis avrBCDEFGHIを発現させて外因性aglyconeと反応させて産物解析。 delta-avrFな発現株では、C-13にD-olivoseが1つbeta-glycoside結合したavermectin A1a3を産生する。 avermectin A1a3を産生できるのはdelta-avrFのみ。 C-3での立体化学は最終的に3-O-methyltransferaseによって決定されるので、AvrF proteinは5-epimeraseであって3,5-epimeraseではない。

Sequence

>putative NDP-hexose epimerase [putative dTDP-4-keto-6-deoxyglucose-2,3-dehydratase]
MDIQELREVPGAYVITPRQWPDPRGTFFESLRTDLVSEAVGRPFEVRQINYSTSRRNTLR
GVHGVLIPPGQAKYVTCVRGALRDMVVDLRVGSPTFGQSASTLLTPENGVAVHVAEGLGH
GFLALTDDTCISYALSTAHVPGTQFEIDPLDPDLALPWGFDEPPLLSAKDAGAPSLRTAL
ERGILPRWLGRIGTP

>putative NDP-hexose epimerase [putative dTDP-4-keto-6-deoxyglucose-2,3-dehydratase]
ATGGACATCCAAGAGCTCCGGGAGGTCCCGGGCGCGTACGTGATCACCCCGCGCCAGTGG
CCGGACCCGCGCGGCACGTTCTTCGAGTCGCTCCGCACCGACCTCGTCAGCGAAGCCGTC
GGCCGCCCTTTCGAAGTGCGGCAGATCAACTACTCGACCTCGCGCCGCAACACGCTGCGT
GGCGTCCACGGCGTGCTGATTCCGCCCGGCCAGGCCAAGTACGTCACCTGTGTGCGAGGC
GCGCTGCGCGACATGGTCGTGGACCTGCGGGTCGGATCACCCACCTTCGGGCAGAGCGCG
AGCACCCTGCTCACCCCGGAGAACGGAGTGGCCGTCCACGTCGCGGAGGGCCTCGGCCAC
GGCTTCCTCGCGCTCACCGACGACACCTGCATCTCCTACGCCCTGTCCACCGCGCATGTG
CCGGGCACCCAGTTCGAGATCGACCCGCTCGACCCCGACCTGGCTCTCCCCTGGGGATTC
GACGAGCCACCGCTGCTGTCCGCGAAGGACGCCGGGGCTCCATCGCTGCGCACCGCTCTG
GAGCGGGGCATCCTGCCGCGCTGGCTCGGAAGGATCGGGACACCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000888 dTDP-4-dehydrorhamnose 3,5-epimerase-related (Family) PF00908 dTDP_sugar_isom PD001462 dTDP_sugar_isom IPR011051 RmlC-like cupin domain (Domain) SSF51182 RmlC_like_cupin IPR014710 RmlC-like jelly roll fold (Domain) G3DSA:2.60.120.10 RmlC-like_jellyroll
SignalP	No significant hit
TMHMM	No significant hit

Grana_00180 Grana_00200