Grana_00190 Grana_00190   Grana_00210 Grana_00210

Grana_00200 : CDS information

close this sectionLocation

Organism
StrainTü22
Entry nameGranaticin
Contig
Start / Stop / Direction23,872 / 22,844 / - [in whole cluster]
23,872 / 22,844 / - [in contig]
Locationcomplement(22844..23872) [in whole cluster]
complement(22844..23872) [in contig]
TypeCDS
Length1,029 bp (342 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductdTDP-hexose C-3 ketoreductase
Product (GenBank)
Gene
Gene (GenBank)gra-orf26
EC number
Keyword
  • D-olivose
  • L-rhodinose
Note
Note (GenBank)
Reference
ACC
PmId
[9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998)
comment
[PMID: 9831526](1998)
entire granaticin gene cluster(gra cluster)の報告。

ORF26: deoxyhexose生合成に関連したoxidoreductasesの可能性あり。
配列解析のみ。

--
[Pubmed登録なし]Mechanism of the 2-Deoxygenation Step in the Biosynthesis of the Deoxyhexose Moieties of the Antibiotics Granaticin and Oleandomycin. J. Am. Chem. Soc. 1999/abstract

gra-orf27 or Tu99-orf10 + dTDP-4-keto-6-deoxyglucose → dTDP + maltol
明らかに酵素反応は不安定な化合物を産生しており、たぶんdTDP-3,4-diketo-2,6-dideoxyglucoseまたはその2,3-enolで、dTDPの容易な除去を起こす。

Gra-orf26 + dTDP-4-keto-6-deoxyglucose + NAD(P)H → 反応なし
Gra-orf26 + dTDP-4-keto-6-deoxyglucose + NAD(P)H + Gra-orf27 → dTDP-4-keto-2,6-dideoxyglucose

Gra-orf27をTu99-orf10で、Gra-orf26をTu99-orf11で置き換えても同じ産物を得た。(Tu99 genesはoleandomycin生合成genes)
NADPHがbetterな還元剤だが、NADHでも代わりできる。

Gra-orf27 または Tu99-orf10は、dTDP-4-keto-6-deoxyglucose 2,3-dehydratasesとして働き、
dTDP-4-keto-6-deoxyglucose → dTDP-3,4-diketo-2,6-dideoxyglucoseへ変換する。
dTDP-3,4-diketo-2,6-dideoxyglucoseは3-ketoreductasesであるGra-orf26 または Tu99-orf11 によって還元的に捕獲され、dTDP-4-keto-2,6-dideoxyglucoseを与える。

close this sectionSequence

selected fasta
>dTDP-hexose C-3 ketoreductase
MNAGHTETVRALRIGVAGCADIALRRMLPAFAASPHTEPTAVASRSSEKARAAAETFGCA
AVEGYDALLERRDVDAVYIPLPVALHAPWTERALRAGKHVLAEKPLTARAADTARLLDLA
RERGLVLAENYLFVHHSAYTAVRDLVDAGAIGDVRALSASFTIPPRSADDIRYRADLDGG
ALLDIGVYPLRLASLLLGSELRVRGAVLRHDTVRGVDLGGSALLGDPGTGVSAQLVFGME
HAYTAGWRLLGSEGSLTLDRAYSPPAGHRPVLRIERPDGTEERILPAHDQATAAVAAFAE
AVRRAGQGAGQDKGRSSGDAAAVLRQAELVDAVRQAAHLVKI
selected fasta
>dTDP-hexose C-3 ketoreductase
GTGAACGCGGGGCACACCGAAACGGTGAGGGCGCTGCGGATCGGGGTCGCGGGCTGTGCC
GACATCGCGCTGCGCCGGATGCTGCCCGCCTTCGCCGCCTCCCCGCACACGGAGCCGACG
GCCGTCGCCAGCCGCAGCTCCGAGAAGGCGCGGGCCGCCGCCGAGACCTTCGGGTGCGCG
GCCGTCGAGGGGTACGACGCCCTCCTCGAACGCCGCGACGTGGACGCCGTGTACATCCCG
CTGCCGGTCGCCCTGCACGCCCCCTGGACCGAGCGCGCCCTGCGCGCGGGCAAGCACGTC
CTCGCTGAGAAGCCGCTGACCGCGCGGGCGGCCGACACCGCACGCCTGCTGGACCTCGCC
CGCGAACGCGGCCTCGTCCTCGCCGAGAACTATCTGTTCGTCCACCACTCCGCCTACACG
GCCGTACGGGACCTGGTGGACGCCGGTGCGATCGGTGATGTGCGGGCGCTGAGCGCCTCG
TTCACCATCCCGCCGCGCTCCGCGGACGACATCCGCTACCGCGCGGACCTCGACGGCGGC
GCGCTCCTCGACATCGGCGTGTACCCGCTCCGGCTGGCCTCTTTGCTGCTCGGCTCCGAG
CTGCGGGTGCGCGGGGCCGTCCTGCGCCACGACACCGTGCGCGGTGTCGACCTCGGCGGC
AGCGCGTTGCTCGGCGACCCCGGCACCGGCGTCAGCGCCCAACTTGTCTTCGGCATGGAG
CACGCCTACACGGCGGGCTGGCGGCTCCTGGGCAGCGAGGGCAGCCTCACCCTCGACCGG
GCGTACTCGCCCCCCGCCGGCCACCGCCCGGTCCTGCGCATCGAGCGTCCGGACGGCACC
GAGGAGCGGATCCTTCCCGCACACGACCAGGCGACCGCCGCCGTCGCCGCCTTCGCCGAA
GCCGTCCGCCGGGCCGGGCAGGGCGCCGGCCAGGACAAGGGCCGGAGCTCGGGCGACGCG
GCGGCGGTGCTCCGCCAGGCCGAGCTGGTCGACGCCGTCCGGCAGGCAGCCCATCTCGTG
AAGATCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000683 Oxidoreductase, N-terminal (Domain)
[12-131] 1.2e-26 PF01408
PF01408   GFO_IDH_MocA
IPR004104 Oxidoreductase, C-terminal (Domain)
[143-248] 1.4e-07 PF02894
PF02894   GFO_IDH_MocA_C
IPR016040 NAD(P)-binding domain (Domain)
[8-153] 5.79999999999997e-44 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-32] 0.421 Signal
Bacteria, Gram-negative   
[1-32] 0.52 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Grana_00190 Grana_00190   Grana_00210 Grana_00210
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