Grana_00210 : CDS information

Location

Organism	Streptomyces violaceoruber
Strain	Tü22
Entry name	Granaticin
Contig	AJ011500
Start / Stop / Direction	25,200 / 23,869 / - [in whole cluster] 25,200 / 23,869 / - [in contig]
Location	complement(23869..25200) [in whole cluster] complement(23869..25200) [in contig]
Type	CDS
Length	1,332 bp (443 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase
Product (GenBank)	putative dTDP-4-keto-6-deoxyglucose-3,5-epimerase
Gene
Gene (GenBank)	gra-orf27
EC number
Keyword	D-olivose L-rhodinose
Note
Note (GenBank)
Reference	ACC Q9ZA32 PmId [9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998) comment UniProtでORF25とORF27のproduct nameが逆に登録されている模様。 --- [PMID: 9831526](1998) entire granaticin gene cluster(gra cluster)の報告。 ORF27: dTDP-4-keto-6-deoxyglucose-2,3-dehydratase 配列解析のみ。 -- [Pubmed登録なし]Mechanism of the 2-Deoxygenation Step in the Biosynthesis of the Deoxyhexose Moieties of the Antibiotics Granaticin and Oleandomycin. J. Am. Chem. Soc. 1999/abst gra-orf27 or Tu99-orf10 + dTDP-4-keto-6-deoxyglucose → dTDP + maltol 明らかに酵素反応は不安定な化合物を産生しており、たぶんdTDP-3,4-diketo-2,6-dideoxyglucoseまたはその2,3-enolで、dTDPの容易な除去を起こす。 Gra-orf26 + dTDP-4-keto-6-deoxyglucose + NAD(P)H → 反応なし Gra-orf26 + dTDP-4-keto-6-deoxyglucose + NAD(P)H + Gra-orf27 → dTDP-4-keto-2,6-dideoxyglucose Gra-orf27をTu99-orf10で、Gra-orf26をTu99-orf11で置き換えても同じ産物を得た。(Tu99 genesはoleandomycin生合成genes) NADPHがbetterな還元剤だが、NADHでも代わりできる。 Gra-orf27 または Tu99-orf10は、dTDP-4-keto-6-deoxyglucose 2,3-dehydratasesとして働き、 dTDP-4-keto-6-deoxyglucose → dTDP-3,4-diketo-2,6-dideoxyglucoseへ変換する。 dTDP-3,4-diketo-2,6-dideoxyglucoseは3-ketoreductasesであるGra-orf26 または Tu99-orf11 によって還元的に捕獲され、dTDP-4-keto-2,6-dideoxyglucoseを与える。

Sequence

>dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase [putative dTDP-4-keto-6-deoxyglucose-3,5-epimerase]
MRITDTAGFHAWFAERGAAHRYRITRTPLHDLEGWYTDPASGDVRHRSGRFFSIEGLRYG
RQEPDGPAWTQPIIRQPETGVLGVLIKWFDGVPHLLMQAKMEPGNINTLQVSPTVQATFS
NYTRVHHGSPVRYIDHFLTPGAGDRVHYDALQSEQGSWFLGKRNRNIVVETTGEIPVHED
FCWVPRPVMAELLRVDNLVNMDSRTVLAGLPDDPGEGSVPRRAVEKPLHDTAALLHWFTG
AKVRHRPERTTIPLSRVGGWRRDDDRGEIVHETGRYFRIIGVDVEADSREVTSWSQPMLA
PVGRGVVAFVSKEIHGERHLLVQARAEAGTFDAVELGPTVQCNPGNLPDGAPRPPYLDTV
LTARPEQVLFDTVHSEEGGRFYHAENRYLVLDGDDVPVDVPEDYTWMTVRQLTRAGRIGN
LVDVEARTLLACVRTLPDHGASR

>dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase [putative dTDP-4-keto-6-deoxyglucose-3,5-epimerase]
ATGCGAATCACGGACACGGCCGGCTTCCATGCCTGGTTCGCCGAGCGGGGCGCGGCCCAC
CGCTACCGCATCACCCGGACGCCGCTGCACGACCTGGAGGGCTGGTACACCGACCCGGCG
AGCGGCGATGTCCGCCACCGCAGCGGGCGGTTCTTCTCCATCGAGGGTCTGCGGTACGGA
CGGCAGGAACCTGACGGGCCCGCATGGACGCAGCCGATCATCCGGCAGCCCGAGACGGGC
GTCCTGGGCGTGCTGATCAAGTGGTTCGACGGGGTTCCGCATCTGCTGATGCAGGCCAAG
ATGGAGCCGGGCAACATCAACACGCTCCAGGTCTCGCCGACCGTGCAGGCCACGTTCAGC
AACTACACACGGGTCCACCACGGTTCCCCCGTGCGCTATATCGACCACTTCCTCACACCG
GGGGCCGGGGACCGGGTCCACTACGACGCGCTGCAGAGCGAGCAGGGATCGTGGTTCCTG
GGCAAGCGCAACCGCAACATCGTCGTGGAGACGACCGGGGAGATCCCGGTACACGAGGAC
TTCTGCTGGGTGCCGCGTCCGGTGATGGCGGAGCTGCTGCGCGTCGACAACCTCGTCAAC
ATGGACTCGCGCACCGTGCTGGCCGGTCTGCCGGACGATCCCGGCGAGGGCTCCGTGCCG
AGGCGGGCGGTGGAGAAGCCGCTGCACGACACCGCGGCGCTGCTGCACTGGTTCACCGGA
GCCAAGGTGCGGCACCGTCCCGAGCGCACGACGATCCCGCTGAGTCGGGTCGGCGGCTGG
CGCCGCGACGACGACCGGGGTGAGATCGTCCATGAGACGGGCCGGTACTTCCGGATCATC
GGCGTCGATGTCGAGGCCGACAGCCGCGAGGTGACGTCCTGGTCGCAGCCGATGCTGGCG
CCGGTCGGCCGGGGAGTGGTCGCCTTCGTCAGCAAGGAGATCCACGGGGAACGCCATCTC
CTGGTGCAGGCCCGCGCGGAGGCGGGGACCTTCGACGCCGTCGAGCTGGGACCCACCGTG
CAGTGCAACCCGGGCAACCTGCCCGACGGCGCACCCCGGCCGCCCTACCTGGACACCGTC
CTGACCGCACGGCCCGAGCAGGTGCTCTTCGACACCGTCCACTCCGAGGAGGGCGGCCGG
TTCTACCACGCGGAGAACCGCTACCTCGTCCTGGACGGAGACGACGTGCCCGTCGACGTA
CCCGAGGACTACACCTGGATGACGGTCCGCCAGCTCACCCGCGCCGGCCGGATCGGCAAC
CTCGTCGACGTGGAGGCGCGCACCCTGCTGGCGTGCGTCCGCACCCTGCCCGACCACGGA
GCATCCCGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005212 NDP-hexose 2,3-dehydratase (Domain) PF03559 Hexose_dehydrat
SignalP	Eukaryota
TMHMM	No significant hit

Grana_00200 Grana_00220