Grana_00300 Grana_00300   Grana_00320 Grana_00320

Grana_00310 : CDS information

close this sectionLocation

Organism
StrainTü22
Entry nameGranaticin
Contig
Start / Stop / Direction33,991 / 34,419 / + [in whole cluster]
33,991 / 34,419 / + [in contig]
Location33991..34419 [in whole cluster]
33991..34419 [in contig]
TypeCDS
Length429 bp (142 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.4 hypothetical protein
Producthypothetical protein
Product (GenBank)
Gene
Gene (GenBank)gra-orf31
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998)
comment
entire granaticin gene cluster(gra cluster)の報告。

ORF31: Unknown(Tailoring?)

配列解析のみ。
actVI-A homologue
mtmX, dpsHとも同じfamily
Related Reference
ACC
Q53925
NITE
Actino_00020
PmId
[17227452] Possible involvement of ActVI-ORFA in transcriptional regulation of actVI tailoring-step genes for actinorhodin biosynthesis. (FEMS Microbiol Lett. , 2007)
comment
Blast 5th, id57%, 5e-36
Streptomyces coelicolor_ORFA(actVI-ORFA)
Hydroxylacyl-CoA dehydrogenase
[Actino_00020]hypothetical protein

actVI-ORFA mutantはactinorhodin産生減少、中間体(S)-DNPAとshunt産物(S)-NHABを蓄積。相補で回復。
また、同じmutantでactVI-ORF1発現量が減少。相補で回復。
よってActVI-ORFAは、post-PKS tailoring enzymeであるactVI-ORF1の発現を通してactinorhodin産生に影響を与えるregulatory roleを持つ可能性あり。

close this sectionSequence

selected fasta
>hypothetical protein
MTATAVRTDVYAQVQHFYAHQMQALDGGRFADYAATFTEDGTFQHSPSAAPAVGRAGIVA
ELVEFNKRFDGDPVQRRHWFNHIALEPQADGSLRSTVYALVVTVRPGGKPEIAPSCVVHD
VLVVTDEGVRTRSRLVTHDQLA
selected fasta
>hypothetical protein
GTGACCGCCACCGCAGTCCGTACCGATGTCTACGCCCAGGTCCAGCACTTCTACGCCCAC
CAGATGCAGGCCCTCGACGGGGGTCGCTTCGCCGACTACGCGGCCACGTTCACGGAGGAC
GGCACCTTCCAGCACTCCCCCTCCGCCGCGCCGGCGGTGGGCCGGGCCGGGATCGTCGCC
GAACTGGTGGAATTCAACAAGCGCTTCGACGGCGACCCGGTCCAGCGGCGTCACTGGTTC
AACCACATCGCCCTGGAGCCCCAGGCCGACGGCTCGCTGCGCTCCACCGTGTACGCCCTG
GTCGTCACCGTCCGCCCCGGCGGGAAGCCGGAGATCGCCCCCAGCTGTGTCGTCCACGAC
GTCCTCGTCGTCACGGACGAGGGCGTACGGACCCGTTCCCGCCTGGTCACCCACGACCAG
CTGGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002075 Nuclear transport factor 2 (Domain)
[14-130] 1.7e-14 PF02136
PF02136   NTF2
SignalP
[1-12] 0.209 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Grana_00300 Grana_00300   Grana_00320 Grana_00320
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