Grana_00320 Grana_00320   Grana_00340 Grana_00340

Grana_00330 : CDS information

close this sectionLocation

Organism
StrainTü22
Entry nameGranaticin
Contig
Start / Stop / Direction35,302 / 36,267 / + [in whole cluster]
35,302 / 36,267 / + [in contig]
Location35302..36267 [in whole cluster]
35302..36267 [in contig]
TypeCDS
Length966 bp (321 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Productputative cyclase/dehydratase
Product (GenBank)cyclase
Gene
Gene (GenBank)gra-orf33
EC number
Keyword
  • 2nd ring
Note
Note (GenBank)
Reference
ACC
PmId
[2583128] Structure and deduced function of the granaticin-producing polyketide synthase gene cluster of Streptomyces violaceoruber Tu22. (EMBO J. , 1989)
[9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998)
comment
[PMID: 2583128](1989)
granaticin生合成gene clusterの一部6.5 kb regionの配列解析報告。

---
[PMID: 9831526](1998)
entire granaticin gene cluster(gra cluster)の報告。

ORF33: Second ring cyclisation (CYC)[tailoring]

配列解析。
actIVに似ている。
Related Reference
ACC
Q9X466
NITE
Naphcy_00060
PmId
[10206788] Isolation and characterization of the naphthocyclinone gene cluster from Streptomyces arenae DSM 40737 and heterologous expression of the polyketide synthase genes. (Gene. , 1999)
comment
Blast 2nd, id71%, 1e-115
Streptomyces arenae__ncnE
Cyclase
[Naphcy_00060]cyclase/dehydratase

NcnE: cyclase
actIVとid55.2%

ncnE + actKR, PKS, ARO → 3,8 DMAC and aloesaponarin II産生
act KR, PKS, ARO, CYCでも同じもの産生。
よってNcnE = act CYC(actIV gene)
ActIVはC5-C14での2nd ring cyclizationを担う。
ACC
Q93IZ0
NITE
Actino_00220
PmId
[11851466] Genetic Contributions to Understanding Polyketide Synthases. (Chem Rev. , 1997)
comment
Blast 13th, id55%, 2e-80
Streptomyces coelicolor_SCO5091
Cyclase
[Actino_00220]cyclase/dehydratase_actIV(actIORF5)

--
[Pubmed登録なし]Engineered Biosynthesis of Novel Polyketides: actVII and actIV Genes Encode Aromatase and Cyclase Enzymes, Respectively. J. Am. Chem. Soc., 1994, 116 (24), pp 10855-10859

act minimal PKS, KR(actIII ), actVII, actIV の様々な組み合わせのrecombinantsによって産生されたpolyketides構造を測定。

minimal PKS = SEK4
minimal PKS + act KR = mutactin
minimal PKS + act KR + actVII = SEK34
minimal PKS + act KR + actVII + actIV = DMAC

ActVII は1st carbocyclic ringの2つのdehydrationsを触媒することを担うaromataseとして機能する。
AcyIV は2つめのringを形成するための分子内aldol縮合を触媒するcyclase(aldolase)である。

ActVII homologsは、gra, fren, tcm, gris生合成clusterにある。
ActIV homologsは、gra, fren, gris生合成clusterにある。

--
[PMID:11851466](1997)
上記PMIDなし論文がRef採用されているレビュー。

close this sectionSequence

selected fasta
>putative cyclase/dehydratase [cyclase]
MTGVTTATAVGATEGAAGGGATGRRPELTEVADGVFAYVQPDGGWCLNNAGLILSGDRAA
LVDTAATETRARGLREAVLSVAPTAPQVLVNTHFHGDHTFGNFVFPEALVVGHERARTEM
TEAGLHLTGLWPDVEWGALALVPPALTFRDRLTLHIGDKAAEVLHLGPAHTSNDTVVWLP
AERVLFTGDLVMNGVTPFCPMGSVAGSLEALDTMRALAPEVVVTGHGPVAGPGVFDETEA
YLRLLQDLARQGLAEKLDPVALARCTDLGRYARWLDAERLVPNLFRAYAEEQGEPRGSRV
DMSELFRRMVEYHGGLPTCHA
selected fasta
>putative cyclase/dehydratase [cyclase]
ATGACCGGAGTGACCACCGCGACGGCCGTCGGAGCGACCGAGGGCGCGGCCGGCGGGGGA
GCGACCGGCCGCCGGCCCGAGCTGACGGAGGTCGCCGACGGGGTGTTCGCCTACGTCCAG
CCGGACGGAGGCTGGTGCCTGAACAACGCCGGCCTGATCCTCTCGGGCGACCGTGCCGCG
CTCGTCGACACCGCGGCGACCGAGACGCGGGCCCGGGGGCTGCGCGAGGCGGTGCTCTCC
GTCGCCCCGACCGCCCCGCAGGTCCTGGTGAACACTCACTTCCACGGCGACCACACCTTC
GGCAACTTCGTCTTCCCGGAGGCGCTCGTCGTCGGCCACGAACGCGCCCGCACGGAGATG
ACCGAGGCGGGCCTGCATCTGACGGGCCTGTGGCCGGACGTCGAGTGGGGTGCGCTGGCA
CTCGTCCCGCCGGCCCTGACCTTCCGCGACCGGCTCACCCTGCACATCGGCGACAAGGCC
GCCGAGGTGCTCCACCTCGGTCCGGCGCACACGAGCAACGACACCGTGGTCTGGCTGCCC
GCCGAGCGGGTGCTGTTCACCGGCGACCTGGTGATGAACGGGGTGACGCCGTTCTGCCCC
ATGGGCTCGGTCGCCGGCTCCCTGGAGGCGCTGGACACGATGCGCGCCCTCGCCCCCGAG
GTGGTCGTCACCGGCCACGGACCGGTCGCGGGGCCCGGGGTGTTCGACGAGACCGAGGCA
TATCTGCGCCTGCTCCAGGATCTCGCCCGACAGGGTCTCGCCGAGAAGCTCGACCCCGTC
GCGCTGGCCCGCTGTACCGACCTGGGCCGGTACGCGCGGTGGCTGGACGCCGAGCGGCTC
GTCCCCAACCTCTTCCGGGCGTACGCCGAGGAACAGGGCGAGCCCCGCGGTTCCCGCGTG
GACATGAGCGAGCTGTTCCGGCGCATGGTGGAGTACCACGGCGGGCTCCCCACCTGCCAC
GCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001279 Beta-lactamase-like (Domain)
[47-226] 3.00000067992871e-32 SM00849
SM00849   Lactamase_B
[44-226] 6.60000000000002e-22 PF00753
PF00753   Lactamase_B
SignalP No significant hit
TMHMM No significant hit
Grana_00320 Grana_00320   Grana_00340 Grana_00340
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