Griseo_00240 : CDS information

Location

Organism	Streptomyces sp.
Strain	JP95
Entry name	Griseorhodin A
Contig	AF509565
Start / Stop / Direction	27,019 / 27,462 / + [in whole cluster] 27,019 / 27,462 / + [in contig]
Location	27019..27462 [in whole cluster] 27019..27462 [in contig]
Type	CDS
Length	444 bp (147 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	GrhN
Gene
Gene (GenBank)	grhN
EC number
Keyword
Note	It is supposed that this ORF is not involved in griseorhodin A biosynthesis.
Note (GenBank)
Reference	ACC Q8KSW8 PmId [12323376] A gene cluster from a marine Streptomyces encoding the biosynthesis of the aromatic spiroketal polyketide griseorhodin A. (Chem Biol. , 2002) [19175308] Cleavage of four carbon-carbon bonds during biosynthesis of the griseorhodin a spiroketal pharmacophore. (J Am Chem Soc. , 2009) comment [PMID:12323376](2002) griseorhodin A生合成gene clusterのクローニング、シークエンス、異種性発現。 GrhN: Unknown 配列解析のみ。 --- [PMID:19175308](2009) grhN mutantでもgriseorhodin A産生可能。

Sequence

>hypothetical protein [GrhN]
MVALLTGPEPGPVAEHLALLHFTGRRSGAAHTVPAGLHRVGGGLFVATGSPWRHNFAGGA
PGEITWRGNRSPVRFTLVTDGERTARGYHELYERYGPEAAQRRLGITVDAAATPTPADFR
AAVTGIPLALVAVDLLTASVTNERTSR

>hypothetical protein [GrhN]
ATGGTCGCCCTGCTGACCGGCCCGGAGCCCGGCCCGGTCGCCGAGCACCTGGCCCTGCTG
CACTTCACCGGTCGGCGCAGCGGCGCGGCGCACACCGTCCCGGCGGGCCTGCACCGCGTC
GGGGGCGGGCTGTTCGTCGCCACCGGCAGCCCCTGGCGGCACAACTTCGCCGGCGGGGCG
CCCGGCGAGATCACCTGGCGCGGCAACCGCTCCCCCGTCCGCTTCACGCTGGTCACCGAC
GGGGAGCGGACCGCCCGCGGCTACCACGAGCTGTACGAGCGCTACGGCCCCGAGGCCGCC
CAGCGCCGCCTGGGCATCACCGTGGACGCCGCCGCCACGCCCACCCCGGCCGACTTCCGG
GCCGCCGTCACCGGCATCCCGCTCGCCCTGGTGGCCGTGGACCTCCTGACCGCATCCGTC
ACGAACGAGAGGACCTCCCGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR016791 Rubromycin-type polyketide biosynthesis protein, GrhN/RubW, predicted (Family) PIRSF021513 GrhN_RubW_prd
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

Griseo_00230 Griseo_00250