first first   Griseu_00020 Griseu_00020

Griseu_00010 : CDS information

close this sectionLocation

Organism
StrainK-63
Entry nameGriseusin
Contig
Start / Stop / Direction190 / 1,458 / + [in whole cluster]
190 / 1,458 / + [in contig]
Location190..1458 [in whole cluster]
190..1458 [in contig]
TypeCDS
Length1,269 bp (422 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase beta-ketoacyl synthase subunit
Product (GenBank)ketosynthase
Gene
Gene (GenBank)
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
  • ORF1
Reference
ACC
PmId
[8169211] Cloning, sequencing, and analysis of the griseusin polyketide synthase gene cluster from Streptomyces griseus. (J Bacteriol. , 1994)
comment
griseusin polyketide synthase gene clusterの報告。

ORF1 for a ketosynthase

act PKS genesに対応しているORF1-ORF4までの5ORFsを2回組み換えしてermEにすると、griseusinA, Bを産生しない。

KSとしているのは配列解析から。
ORF1(KS), ORF2(CLF)で系統樹作成あり。
Related Reference
ACC
Q54173
NITE
Urd_00120
PmId
[7592377] Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tu2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation. (J Bacteriol. , 1995)
comment
Blast 7th, id75%, 0.0
Streptomyces fradiae_urdA
Putative ketoacyl synthase

urdEFABCDを含むcosmid purd8をS.lividans TK24へ導入すると、urdamycin抵抗性を獲得。
urdamycin産生株S.fradiae Tu2717染色体から、urdE-C末+urdFABを含むfragment g領域を削除するとurdamycin産生不可。

AA配列解析で、UrdAはPKS gene clustersのbeta-ketoacyl synthasesに高い類似性あり。
UrdAとUrdBはid36%で似ているが、UrdBにはないactive site cysteineをUrdAは持っている。

TcmK ketoacyl synthase or TcmL chain length factorのどちらかを傷害してtetracenomycin C非産生にしたStreptomyces glaucescensのmutantで、urdFABを異種性発現したらどちらのmutantも相補され、tetracenomycin Cを産生できた。

close this sectionPKS/NRPS Module

KS3..375

close this sectionSequence

selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [ketosynthase]
MERRAVITGIGVCAPGGTGVDSFWELMCDGRTATRGITLFDPSPYRSRVAAEIDFDPRAH
GLTPQEIRRMDRAAQFGVVTAREAFADSGLDGAALDPFRTGVSLGTAIGAAGGLDAEYRV
VSDDGRLELVDHRYATPHLYDLFVPSSFATEVAWAVGAEGPATVVSTGCTAGIDAVGHAV
EAIRDGSADVMVTGAVDAPITPLTMAAFDAIRATTSLNDEADTASRPFDASRKGFVLGEG
AAVFVLEEAEHARRRGAYVYAEIAGYASRGNAFHMTGLRSDGAELAAAIRAALDEARLDA
SAVDYVNAHGSGTRQNDRHETVALKSALGQHAHRVPVSAIKSMVGHSLGAIGSIEIAACA
LAMRHHVIPPTANLHTPDPECDLDYVPLTARAQRTDAVLTIGSGFGGFQSAMVLARPDRS
AA
selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [ketosynthase]
GTGGAACGCCGGGCAGTGATCACGGGAATCGGTGTGTGCGCACCGGGCGGAACCGGTGTG
GACAGCTTCTGGGAGCTGATGTGCGACGGCCGGACGGCCACCCGCGGCATCACCCTCTTC
GACCCGTCTCCGTACCGCTCCAGGGTCGCCGCCGAGATCGACTTCGACCCGCGGGCGCAC
GGGCTGACGCCGCAGGAGATCCGGCGGATGGACCGGGCCGCGCAGTTCGGCGTGGTGACG
GCACGGGAGGCCTTCGCCGACAGCGGGCTCGACGGCGCCGCGCTCGATCCGTTCCGTACG
GGAGTCAGCCTCGGCACCGCGATCGGCGCGGCCGGCGGCCTGGACGCGGAGTACCGCGTG
GTCAGCGACGACGGCCGGCTGGAGCTGGTCGACCACCGGTACGCGACCCCGCATCTGTAC
GACCTGTTCGTGCCGAGCTCCTTCGCGACCGAAGTGGCCTGGGCCGTCGGCGCGGAAGGA
CCGGCGACGGTGGTGTCCACCGGCTGCACCGCCGGGATCGACGCGGTGGGCCACGCGGTG
GAGGCGATCCGCGACGGCAGCGCCGATGTGATGGTCACCGGCGCGGTGGACGCGCCGATC
ACCCCGCTCACGATGGCGGCCTTCGACGCGATCCGCGCCACCACGTCCCTCAACGACGAG
GCGGACACCGCCTCCCGCCCCTTCGACGCGTCACGCAAGGGCTTCGTCCTCGGTGAGGGG
GCGGCGGTGTTCGTCCTGGAGGAGGCGGAGCACGCACGGCGCAGAGGCGCGTACGTGTAC
GCGGAGATCGCCGGATACGCCTCGCGCGGCAACGCCTTCCACATGACCGGACTGCGGTCC
GACGGAGCGGAGCTGGCCGCCGCGATCCGGGCGGCGCTGGACGAGGCCCGGCTCGACGCG
TCGGCGGTGGACTACGTCAACGCGCACGGTTCCGGCACCCGCCAGAACGACCGGCACGAG
ACGGTCGCGCTCAAGTCGGCTCTGGGACAGCACGCCCACCGGGTGCCGGTCAGCGCGATC
AAGTCGATGGTCGGGCACTCGCTCGGGGCGATCGGCTCCATCGAGATCGCCGCCTGCGCA
CTGGCCATGCGGCACCACGTCATCCCTCCGACCGCCAATCTGCACACCCCGGATCCCGAG
TGCGATCTCGACTATGTCCCGCTGACGGCCCGCGCGCAGCGCACCGATGCCGTCCTCACC
ATCGGCAGCGGATTCGGCGGCTTCCAGAGCGCGATGGTGCTGGCCCGTCCCGACAGGAGC
GCCGCGTGA
KS3..375
KS7..1125

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[3-251] 1.5e-61 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[260-375] 7.69999999999997e-38 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[2-265] 2.39999999999997e-66 G3DSA:3.40.47.10 [270-417] 2.99999999999998e-51 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[2-257] 5.10003242202156e-69 SSF53901 [219-418] 6.39998633403535e-64 SSF53901
SSF53901   Thiolase-like
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[160-176] PS00606
PS00606   B_KETOACYL_SYNTHASE
SignalP
[1-19] 0.644 Signal
Eukaryota   
TMHMM No significant hit
first first   Griseu_00020 Griseu_00020
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