Griseu_00010 Griseu_00010   Griseu_00030 Griseu_00030

Griseu_00020 : CDS information

close this sectionLocation

Organism
StrainK-63
Entry nameGriseusin
Contig
Start / Stop / Direction1,455 / 2,693 / + [in whole cluster]
1,455 / 2,693 / + [in contig]
Location1455..2693 [in whole cluster]
1455..2693 [in contig]
TypeCDS
Length1,239 bp (412 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase chain length factor subunit
Product (GenBank)putative chain length determination factor
Gene
Gene (GenBank)
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
  • ORF2
Reference
ACC
PmId
[8169211] Cloning, sequencing, and analysis of the griseusin polyketide synthase gene cluster from Streptomyces griseus. (J Bacteriol. , 1994)
comment
griseusin polyketide synthase gene clusterの報告。

ORF2 for a chain length-determining factor

act PKS genesに対応しているORF1-ORF4までの5ORFsを2回組み換えしてermEにすると、griseusinA, Bを産生しない。

CLFとしているのは配列解析から。
ORF1(KS), ORF2(CLF)で系統樹作成あり。
Related Reference
ACC
Q54174
NITE
Urd_00130
PmId
[7592377] Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tu2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation. (J Bacteriol. , 1995)
comment
Blast 8th, id62%, 1e-135
Streptomyces fradiae_urdB
Putative chain length determinant

urdEFABCDを含むcosmid purd8をS.lividans TK24へ導入すると、urdamycin抵抗性を獲得。
urdamycin産生株S.fradiae Tu2717染色体から、urdE-C末+urdFABを含むfragment g領域を削除するとurdamycin産生不可。

AA配列解析で、UrdBは芳香族PKS gene clustersのCLFに似ている。
UrdAとUrdBはid36%で似ているが、UrdBはactive site cysteineを持っていない。

TcmK ketoacyl synthase or TcmL chain length factorのどちらかを障害してtetracenomycin C非産生にしたStreptomyces glaucescensのmutantで、urdFABを異種性発現したらどちらのmutantも相補され、tetracenomycin Cを産生できた。

close this sectionPKS/NRPS Module

CLF13..368

close this sectionSequence

selected fasta
>polyketide synthase chain length factor subunit [putative chain length determination factor]
MSAPGGGDRGRTLITGMGLATPHGVDVEDFWAATRVGKNAIGPVTRFDASGYPARLAGEI
RGFSAADHLPGRLVAQTDRVTQLALVAADRAFRDAGVAPGDLPANGMGVVTAAGSGGFEF
GERELRKLWSLGANHVSAYQSFAWFPTANTGQIAIRSDSRGPNGVVVGDQAGGLDALGQA
RRLIRRGTGLVAAGGFEASLTPLGWTARLSTGLMSTSDDPDRAYLPFHPAARGYVPGEGG
ALLILEDERSALARGAATAHAELAGYSATLDPRPGSGREPGLRRAIELALADAGVAPAEV
DVVFADAAGSPEPDAVEVAAVAGVFGPRAVPVTAPKTMIGRLQAGGAPVDVVTAVLAIRE
GLIPPTADAESATARELDLVVGRPRTASVGTALVLARGHGGFNSAVVLRAVD
selected fasta
>polyketide synthase chain length factor subunit [putative chain length determination factor]
GTGAGCGCCCCGGGCGGCGGCGACCGCGGCCGCACGTTGATCACCGGCATGGGACTGGCC
ACACCGCACGGCGTCGACGTGGAGGATTTCTGGGCGGCGACACGGGTCGGCAAGAACGCC
ATCGGACCGGTCACCCGGTTCGACGCGTCCGGCTACCCGGCGCGGCTGGCGGGAGAGATC
CGCGGATTCTCGGCCGCGGACCATCTGCCGGGCCGGCTGGTGGCGCAGACCGACCGGGTG
ACCCAACTGGCCCTGGTCGCCGCGGACCGCGCGTTCCGGGACGCCGGGGTGGCTCCGGGC
GACCTTCCCGCGAACGGGATGGGCGTGGTCACGGCGGCCGGTTCCGGCGGTTTCGAGTTC
GGCGAGCGGGAGTTGCGCAAGCTCTGGAGCCTCGGCGCGAACCACGTGAGCGCCTACCAG
TCGTTCGCCTGGTTCCCGACCGCCAACACCGGCCAGATCGCGATCCGCAGCGACAGCCGC
GGACCGAACGGCGTGGTCGTCGGCGACCAGGCCGGCGGCCTCGACGCGCTCGGACAGGCC
CGGCGGCTCATCCGCCGCGGCACCGGACTGGTCGCCGCGGGCGGCTTCGAGGCGTCGCTG
ACACCGCTCGGCTGGACGGCACGGCTGTCGACCGGACTGATGTCGACCAGCGACGATCCC
GACCGCGCCTATCTGCCGTTCCATCCGGCGGCGCGCGGCTACGTTCCCGGCGAGGGCGGC
GCACTGCTGATCCTGGAGGACGAGCGCAGCGCCCTGGCCCGCGGCGCGGCCACCGCCCAC
GCCGAACTGGCCGGGTACAGCGCGACGCTGGATCCGCGCCCCGGCAGCGGGCGCGAGCCC
GGTCTGCGGCGCGCCATCGAACTGGCGCTGGCGGACGCCGGCGTCGCACCGGCCGAGGTG
GACGTCGTCTTCGCCGACGCCGCCGGCTCACCGGAGCCGGACGCGGTGGAAGTGGCGGCC
GTGGCCGGTGTGTTCGGACCGCGCGCGGTGCCGGTCACCGCGCCGAAGACCATGATCGGC
CGGCTCCAGGCGGGCGGCGCCCCGGTGGACGTGGTGACCGCCGTCCTCGCCATCCGCGAA
GGCCTGATCCCGCCGACCGCCGACGCGGAGTCCGCCACGGCCCGCGAGCTGGACCTGGTC
GTCGGACGGCCCCGGACCGCCTCGGTCGGCACCGCGCTGGTGCTCGCGCGAGGGCACGGC
GGATTCAACTCGGCCGTCGTGCTGCGGGCCGTCGACTGA
CLF13..368
CLF37..1104

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[13-248] 2.50000000000001e-52 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[260-368] 7.5e-26 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[11-264] 3.2e-51 G3DSA:3.40.47.10 [278-411] 6.6999999999999e-36 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[9-256] 9.80000272130214e-59 SSF53901 [219-411] 1.60000240695997e-43 SSF53901
SSF53901   Thiolase-like
SignalP No significant hit
TMHMM No significant hit
Griseu_00010 Griseu_00010   Griseu_00030 Griseu_00030
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