Griseu_00020 Griseu_00020   Griseu_00040 Griseu_00040

Griseu_00030 : CDS information

close this sectionLocation

Organism
StrainK-63
Entry nameGriseusin
Contig
Start / Stop / Direction2,809 / 3,078 / + [in whole cluster]
2,809 / 3,078 / + [in contig]
Location2809..3078 [in whole cluster]
2809..3078 [in contig]
TypeCDS
Length270 bp (89 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase acyl carrier protein subunit
Product (GenBank)acyl carrier protein
Gene
Gene (GenBank)
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
  • ORF3
Reference
ACC
PmId
[8169211] Cloning, sequencing, and analysis of the griseusin polyketide synthase gene cluster from Streptomyces griseus. (J Bacteriol. , 1994)
[9108302] Post-translational modification of heterologously expressed Streptomyces type II polyketide synthase acyl carrier proteins. (FEBS Lett. , 1997)
[9738947] Acylation of Streptomyces type II polyketide synthase acyl carrier proteins. (FEBS Lett. , 1998)
comment
[PMID:8169211](1994)
griseusin polyketide synthase gene clusterの報告。

ORF3 for an acyl carrier protein

act PKS genesに対応しているORF1-ORF4までの5ORFsを2回組み換えしてermEにすると、griseusinA, Bを産生しない。

ACPとしているのは配列解析から。

--
[PMID:9108302](1997)
E.coliにおけるactinorhodin (act), oxytetracycline (otc) and griseusin (gris) apo-ACPsのholo型形成に関する調査。

--
[PMID:9738947](1998)
上記論文の続報。
holo-ACPsによるacylationの調査。
Related Reference
ACC
Q56165
NITE
Jado_00100
PmId
[7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
Blast 5th, id75%, 1e-29
Streptomyces venezuelae_acp
Acyl carrier protein

acp=ORF3=JadC

--
[PMID:7881555](1994)
ORF3は配列解析からtype II PKSのACPであると提唱されている。
ORF2-ORF4領域を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。

--
[PMID: 9224566](1997)
minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。
jadCもACPとして使用されている。

close this sectionPKS/NRPS Module

ACP7..80

close this sectionSequence

selected fasta
>polyketide synthase acyl carrier protein subunit [acyl carrier protein]
MSKQEFTLEDLKRILLEGAGADEGVDLDGDILDTDFEELGYESLALLETGGRIEREYGIT
LDDDVLADSRTPRSLIAAINAAFQGLVEA
selected fasta
>polyketide synthase acyl carrier protein subunit [acyl carrier protein]
GTGTCCAAGCAGGAGTTCACCCTCGAAGACCTCAAGCGCATCCTTCTGGAAGGCGCCGGC
GCGGACGAGGGCGTCGACCTCGACGGCGACATCCTCGACACCGATTTCGAGGAACTCGGC
TACGAGTCGCTGGCGTTGCTGGAGACCGGCGGCCGGATCGAGCGCGAGTACGGGATCACG
CTCGACGACGACGTGCTCGCCGACAGCCGCACGCCGCGCTCGCTGATAGCCGCCATCAAC
GCCGCGTTCCAGGGCCTCGTCGAGGCCTGA
ACP7..80
ACP19..240

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006162 Phosphopantetheine attachment site (PTM)
[38-53] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[3-82] 8.80000265836278e-15 SSF47336
SSF47336   ACP_like
[4-82] 7.59999999999998e-17 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[31-79] 6.40000000000001e-10 PF00550
PF00550   PP-binding
[7-80] PS50075
PS50075   ACP_DOMAIN
SignalP
[1-21] 0.059 Signal
Eukaryota   
TMHMM No significant hit
Griseu_00020 Griseu_00020   Griseu_00040 Griseu_00040
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