Griseu_00040 : CDS information

Location

Organism	Streptomyces griseus
Strain	K-63
Entry name	Griseusin
Contig	X77865
Start / Stop / Direction	3,201 / 3,986 / + [in whole cluster] 3,201 / 3,986 / + [in contig]
Location	3201..3986 [in whole cluster] 3201..3986 [in contig]
Type	CDS
Length	786 bp (261 aa)

Annotation

Category	3.3 modification reduction
Product	putative polyketide C-9 ketoreductase
Product (GenBank)	ketoreductase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	ORF5
Reference	ACC Q54220 PmId [8169211] Cloning, sequencing, and analysis of the griseusin polyketide synthase gene cluster from Streptomyces griseus. (J Bacteriol. , 1994) comment griseusin polyketide synthase gene clusterの報告。 ORF5 for a ketoreductase KRとしているのは配列解析(actIII領域との相同性)から。 act PKS genesに対応しているORF1-ORF4までの5ORFsを2回組み換えしてermEにすると、griseusinA, Bを産生しない。
Related Reference	ACC Q56166 NITE Jado_00110 PmId [7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994) [9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997) comment Blast 8th, id86%, 1e-126 Streptomyces venezuelae Ketoreductase [DoBISCUIT]C-9 ketoreductase_JadE(ORF5) -- [PMID:7881555](1994) ORF5は配列解析からketoreductaseであると提唱されている。 ORF2-ORF4領域(ORF5も含まれる)を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。 -- [PMID: 9224566](1997) minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。 JadEはdecaketideだけでなく、octaketideも還元できる。 DpsE KRはdecaketidesを還元できるが、octaketidesを還元できない。 JadD, DpsFの両cyclasesはC-9で還元されたdecaketidesの位置特異的なfirst-ring cyclase/dehydrasesである。

Sequence

>putative polyketide C-9 ketoreductase [ketoreductase]
MSDQDKRVAVVTGATSGIGLSVARLLASQGHRVFIGARSAENVAATVKELVAEGLDVDGA
VVDVRSDADVEAWIQAAVDRFGTVDVVVNNAGRSGGGVTADIDPELWHDVIDTNLNSVFR
VTRSALNTGGLRSKSRGRIINIASTAGKQGVVLGAPYSASKHGVVGFTKALGNELAPTGI
TVNAVCPGYVETPMAQRVRQGYAAAYDATEDAILEKFQAKIPLGRYSTPDEVAGLVGYLA
SDTAASITSQALNVCGGLGNF

>putative polyketide C-9 ketoreductase [ketoreductase]
ATGTCGGATCAGGACAAGCGCGTCGCCGTCGTCACCGGAGCCACCAGCGGCATCGGACTG
TCCGTCGCGCGCCTGCTGGCCTCACAGGGCCACCGTGTGTTCATCGGCGCGCGCAGCGCG
GAGAACGTCGCCGCGACCGTCAAGGAGCTCGTGGCCGAGGGCCTCGACGTCGACGGCGCG
GTGGTGGACGTCCGCAGCGACGCCGATGTCGAGGCCTGGATCCAGGCCGCCGTGGACCGC
TTCGGCACCGTCGACGTCGTCGTCAACAACGCGGGCCGCTCCGGCGGCGGCGTCACCGCG
GACATCGACCCGGAGCTGTGGCACGACGTCATCGACACCAATCTCAACTCGGTCTTCCGC
GTCACGCGTTCGGCGCTGAACACCGGCGGCCTGCGGTCCAAGAGCCGCGGCCGGATCATC
AACATCGCCTCCACGGCCGGCAAGCAGGGTGTGGTGCTCGGTGCGCCGTACTCCGCGTCC
AAGCACGGCGTGGTCGGCTTCACCAAGGCGCTCGGCAACGAGCTGGCGCCGACCGGCATC
ACCGTCAACGCCGTCTGCCCCGGATACGTCGAGACGCCGATGGCGCAGCGCGTGCGGCAG
GGGTACGCCGCGGCGTACGACGCCACCGAGGACGCGATCCTGGAGAAGTTCCAGGCGAAG
ATCCCGCTCGGCCGCTACTCCACGCCGGACGAGGTCGCCGGACTCGTCGGCTACCTGGCC
TCGGACACCGCGGCCTCCATCACCTCGCAGGCGCTCAACGTCTGCGGCGGACTCGGCAAC
TTCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002198 Short-chain dehydrogenase/reductase SDR (Family) PIRSF000126 11-beta-HSD1 PR00080 SDRFAMILY PF00106 adh_short IPR002347 Glucose/ribitol dehydrogenase (Family) PR00081 GDHRDH IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site) PS00061 ADH_SHORT
SignalP	Eukaryota Bacteria, Gram-positive
TMHMM	No significant hit

Griseu_00030 Griseu_00050