first first   Hecto_00020 Hecto_00020

Hecto_00010 : CDS information

close this sectionLocation

Organism
StrainJHB
Entry nameHectochlorin
Contig
Start / Stop / Direction2,444 / 4,264 / + [in whole cluster]
2,444 / 4,264 / + [in contig]
Location2444..4264 [in whole cluster]
2444..4264 [in contig]
TypeCDS
Length1,821 bp (606 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.4 Other
Productputative carboxylic acid--ACP ligase
putative acyl-ACP synthetase
Product (GenBank)HctA
GenehctA
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • acyl-ACP synthetase
Reference
ACC
PmId
[18001088] Cloning and biochemical characterization of the hectochlorin biosynthetic gene cluster from the marine cyanobacterium Lyngbya majuscula. (J Nat Prod. , 2007)
comment
cyanobacterium Lyngbya majusculaのジャマイカ分離株(strain JHB)から、hectochlorin生合成gene cluster(hct)を同定。

HctA (606aa): acyl-ACP synthetase

配列解析のみ。
JamAと配列がとても似ていることから、free hexanoic acidをATP依存で活性化してhectochlorin生合成を開始すると提唱される。その後acyl基は、acyl-adenylateからHctBのC末領域にあるACP domainへと移動されるようだ。
Related Reference
ACC
Q6E7K9
PmId
[15217615] Structure and biosynthesis of the jamaicamides, new mixed polyketide-peptide neurotoxins from the marine cyanobacterium Lyngbya majuscula. (Chem Biol. , 2004)
comment
Blast 2nd, id61%, 0.0
Lyngbya majuscula
JamA

jamaicamides A-Cを分離し、構造解明、生物学的特性の調査。
さらにjamaicamides生合成gene clusterを同定。

JamA (592aa): hexanoyl-ACP synthetase

His fusion proteinとして過剰発現、精製したJamAを用いて、7つの基質に対するATP-PPi Exchange活性測定を実施。5-hexynoic and 5-hexenoic acidを基質として著しく好んだ。

close this sectionPKS/NRPS Module

0
A41..488

close this sectionSequence

selected fasta
>putative carboxylic acid--ACP ligase [HctA]
MNTKKKFTSLIHLLCYRGTNKPNQKAYTFIGNGKTETASLTYGELEKRSRAIAAQLQEMG
VTRGERALLLYSQPLDFICAFFGCLYAGVIAIPAPPPDAIRLKRTLPRLQACVKDAQVSL
VLTTSQIYSQFPSEWQKDFEYNNMLLWLFTEEISEQLANQWQELKINLDAIAYLQYTSGS
TSTPKGVIVTHNNVMHHSAYIKQAWNYTSDSIAATWMPYFHDYGLIDGLIQPIYSGITCY
VMSPLTFVRRPTCWLEVISKYKVTHSQSPNFGYDYCVRQVTSEQLNNLDLRSWKTASNGA
EPIRKDTIEKFIKTFEPCGFRATAFFPSYGLAEATLLVATKSHNDVPEIASIAASALEKN
QIVECDGNQKGTRYVVSCGFPICGIKVIIINPNTLTRCASDEVGEIWVSDLSVAQGYWNR
PEETKHTFEAYLADTGEGPFLRTGDLGFIKNGQLFVTGRLKDVIIIRGQNHYPQDLEFTV
EQSHPALRKNSEAAFGIEVDGEEKLVVVQEVERSWLRKLDFDQVNGDIRQALMEQHELQV
YAIALIKPGSIPKTSSGKIMRHACRIKFLEGTLEVINSGGGNPEHLRRLTVMSVMRATPK
NGAHLG
selected fasta
>putative carboxylic acid--ACP ligase [HctA]
ATGAATACTAAGAAAAAATTTACCTCTTTGATACACCTGCTATGTTATAGAGGAACAAAT
AAACCGAACCAAAAAGCCTATACCTTTATTGGTAATGGAAAAACAGAGACAGCCAGCTTG
ACTTATGGAGAATTGGAAAAGCGGTCACGAGCAATCGCAGCTCAGCTTCAGGAGATGGGG
GTAACCAGGGGGGAACGAGCATTGCTGCTCTATTCACAGCCTCTAGATTTTATTTGTGCT
TTCTTTGGATGTTTATATGCAGGAGTTATTGCTATTCCGGCTCCTCCACCTGATGCAATT
CGATTGAAGCGAACTCTGCCTCGACTTCAAGCTTGTGTTAAGGATGCCCAAGTTTCTCTA
GTGCTGACTACATCTCAAATTTATTCTCAGTTCCCAAGTGAATGGCAAAAAGACTTTGAG
TATAATAATATGTTGTTATGGTTGTTTACTGAGGAGATATCAGAGCAACTAGCAAACCAA
TGGCAAGAATTAAAAATTAATCTAGATGCCATAGCTTATTTACAGTATACTTCTGGGTCT
ACATCTACACCGAAAGGAGTCATAGTGACCCATAACAATGTAATGCACCATTCTGCCTAT
ATCAAGCAAGCATGGAACTACACTTCAGATAGTATTGCTGCTACTTGGATGCCTTACTTC
CATGATTATGGTTTGATTGATGGACTTATACAGCCTATTTATTCTGGTATTACTTGTTAC
GTAATGTCACCATTGACATTTGTGAGAAGACCGACCTGCTGGCTAGAGGTTATTTCTAAG
TATAAGGTAACCCATAGCCAAAGTCCAAACTTTGGCTATGACTACTGTGTTCGTCAAGTT
ACATCAGAACAATTAAATAATTTAGATCTTAGGAGTTGGAAAACAGCTAGTAATGGAGCA
GAGCCAATTCGTAAAGACACTATAGAAAAATTTATTAAAACATTTGAACCTTGTGGGTTC
CGTGCCACAGCTTTTTTTCCATCTTATGGTCTTGCAGAAGCAACTTTGTTAGTTGCTACC
AAGTCTCATAATGATGTGCCTGAAATTGCATCAATTGCTGCATCAGCACTAGAGAAAAAT
CAGATTGTTGAGTGCGATGGTAATCAAAAGGGAACTCGATATGTGGTTAGTTGTGGTTTC
CCGATTTGTGGAATAAAAGTAATTATCATCAATCCCAATACTTTAACTAGGTGTGCGTCA
GATGAAGTTGGCGAAATTTGGGTATCAGATTTGAGTGTTGCTCAAGGTTATTGGAATAGA
CCAGAAGAAACAAAGCATACATTTGAGGCATATCTAGCAGATACTGGTGAAGGTCCATTT
CTACGTACTGGTGACTTGGGGTTCATCAAGAATGGTCAGCTGTTTGTCACTGGGCGCTTG
AAGGATGTAATCATCATTCGAGGTCAAAATCATTATCCTCAGGATCTTGAATTTACGGTT
GAACAGAGCCACCCAGCACTGCGTAAAAATTCTGAGGCAGCATTTGGGATTGAGGTAGAT
GGCGAAGAGAAATTAGTCGTTGTTCAGGAGGTGGAGCGAAGTTGGCTGCGAAAACTGGAT
TTCGATCAAGTAAATGGAGATATTCGTCAGGCGTTGATGGAGCAACACGAACTACAGGTT
TATGCAATAGCATTGATCAAACCAGGTAGCATTCCTAAAACTTCAAGTGGCAAGATAATG
CGTCACGCTTGTCGGATTAAGTTTCTAGAGGGAACTTTGGAAGTTATAAATTCAGGTGGT
GGTAATCCAGAACACTTAAGAAGACTAACTGTTATGAGTGTTATGAGGGCAACTCCAAAA
AATGGGGCGCATCTGGGGTAG
[0] A41..488
[0] A121..1464

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[41-488] 9.60000000000004e-89 PF00501
PF00501   AMP-binding
IPR020845 AMP-binding, conserved site (Conserved_site)
[174-185] PS00455
PS00455   AMP_BINDING
SignalP No significant hit
TMHMM No significant hit
first first   Hecto_00020 Hecto_00020
Page top